Strain Name:
C57BL/6J-MtgxR3818Btlr/Mmmh
Stock Number:
040772-MU
Citation ID:
RRID:MMRRC_040772-MU
Other Names:
R3818 (G1), C57BL/6J-MtgxR3818Btlr
Major Collection:

Strain Information

Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Gpr21
Name: G protein-coupled receptor 21
Synonyms: C230004C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 338346
HGNC: HGNC:4476
Homologene: 74546
Zfp939
Name: zinc finger protein 939
Synonyms: 9430025M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233147
Homologene: 136498
Rbm26
Name: RNA binding motif protein 26
Synonyms: Pro1777, C230097K14Rik, 1700009P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74213
Homologene: 41468
Pikfyve
Name: phosphoinositide kinase, FYVE type zinc finger containing
Synonyms: 5230400C17Rik, Pip5k3, PipkIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18711
Homologene: 32115
2310011J03Rik
Name: RIKEN cDNA 2310011J03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66374
Homologene: 11959
Pwp1
Name: PWP1 homolog, endonuclein
Synonyms: 2610205J09Rik, 2310058A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103136
Homologene: 5129
Tlr4
Name: toll-like receptor 4
Synonyms: Lps, Rasl2-8, lipopolysaccharide response
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21898
Homologene: 41317
Pah
Name: phenylalanine hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18478
HGNC: HGNC:8582
Homologene: 234
Dse
Name: dermatan sulfate epimerase
Synonyms: B130024B19Rik, Sart2, DS-epi1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212898
VEGA: 10
Homologene: 8354
Cachd1
Name: cache domain containing 1
Synonyms: 1190007F10Rik, Vwcd1, B430218L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 320508
Homologene: 10854
Pomgnt1
Name: protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase
Synonyms: 0610016I07Rik, 4930467B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68273
Homologene: 9806
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Skint5
Name: selection and upkeep of intraepithelial T cells 5
Synonyms: OTTMUSG00000008560
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242627
Homologene: 135888
Gsdme
Name: gasdermin E
Synonyms: 4932441K13Rik, 2310037D07Rik, Fin15, Dfna5h, Dfna5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 54722
HGNC: HGNC:2810
Homologene: 3242
Adra2b
Name: adrenergic receptor, alpha 2b
Synonyms: [a]2B, alpha2B, Adra-2b, a2b-AR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11552
HGNC: HGNC:282
Homologene: 553
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21974
Homologene: 134711
Plekhn1
Name: pleckstrin homology domain containing, family N member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 231002
VEGA: 4
Homologene: 12949
Mamdc4
Name: MAM domain containing 4
Synonyms: LOC381352
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 381352
Homologene: 17102
Or8i2
Name: olfactory receptor family 8 subfamily I member 2
Synonyms: GA_x6K02T2Q125-48508763-48507833, MOR207-1, Olfr1104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258763
Homologene: 64916
Wdhd1
Name: WD repeat and HMG-box DNA binding protein 1
Synonyms: AND-1, D630024B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218973
Homologene: 56019
Gabrg3
Name: gamma-aminobutyric acid (GABA) A receptor, subunit gamma 3
Synonyms: Gabrg-3, B230362M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14407
HGNC: HGNC:4088
Homologene: 22444
Tnfrsf11a
Name: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Synonyms: Rank, TRANCE-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21934
Homologene: 2848
Prkd1
Name: protein kinase D1
Synonyms: Pkcm, PKD1, Prkcm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18760
VEGA: 12
HGNC: HGNC:9407
Homologene: 55680
Cyp4a12b
Name: cytochrome P450, family 4, subfamily a, polypeptide 12B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 13118
Homologene: 134044
Ttl
Name: tubulin tyrosine ligase
Synonyms: 2410003M22Rik, 2700049H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69737
Homologene: 32678
Sorcs3
Name: sortilin-related VPS10 domain containing receptor 3
Synonyms: 6330404A12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66673
VEGA: 19
Homologene: 8986
Cd177
Name: CD177 antigen
Synonyms: Pdp3, 1190003K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68891
Homologene: 49628
Col25a1
Name: collagen, type XXV, alpha 1
Synonyms: 2700062B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 77018
Homologene: 57111
Or5p59
Name: olfactory receptor family 5 subfamily P member 59
Synonyms: GA_x6K02T2PBJ9-10432095-10433042, MOR204-12, Olfr483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258730
Homologene: 133602
Rasa4
Name: RAS p21 protein activator 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54153
Homologene: 5080
Gjb2
Name: gap junction protein, beta 2
Synonyms: connexin 26, Cx26, Gjb-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14619
HGNC: HGNC:4284
Homologene: 2975
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 65,245,758 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 1 at 105,809,360 bp
  • C to T, chromosome 2 at 25,565,773 bp
  • C to A, chromosome 2 at 37,518,312 bp
  • G to A, chromosome 2 at 87,021,710 bp
  • G to T, chromosome 2 at 127,363,835 bp
  • T to C, chromosome 2 at 129,092,994 bp
  • A to G, chromosome 3 at 130,550,071 bp
  • A to G, chromosome 4 at 66,841,316 bp
  • A to T, chromosome 4 at 100,990,865 bp
  • A to G, chromosome 4 at 113,629,122 bp
  • A to T, chromosome 4 at 115,432,470 bp
  • T to A, chromosome 4 at 116,153,942 bp
  • T to C, chromosome 4 at 156,225,533 bp
  • A to G, chromosome 5 at 136,102,293 bp
  • A to T, chromosome 6 at 48,481,103 bp
  • A to C, chromosome 6 at 50,219,411 bp
  • A to G, chromosome 7 at 24,754,392 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • T to C, chromosome 7 at 39,473,368 bp
  • G to A, chromosome 7 at 57,381,664 bp
  • A to G, chromosome 7 at 108,103,498 bp
  • C to T, chromosome 8 at 16,002,522 bp
  • T to C, chromosome 10 at 14,143,941 bp
  • T to C, chromosome 10 at 34,153,433 bp
  • T to C, chromosome 10 at 80,319,517 bp
  • C to T, chromosome 10 at 85,888,129 bp
  • A to G, chromosome 10 at 87,522,004 bp
  • A to T, chromosome 12 at 50,419,884 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • A to G, chromosome 14 at 47,243,801 bp
  • A to G, chromosome 14 at 57,100,073 bp
  • A to T, chromosome 14 at 105,141,270 bp
  • A to G, chromosome 19 at 48,603,904 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3818 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040772-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.