Strain Name:
C57BL/6J-MtgxR3852Btlr/Mmmh
Stock Number:
040784-MU
Citation ID:
RRID:MMRRC_040784-MU
Other Names:
R3852 (G1), C57BL/6J-MtgxR3852Btlr
Major Collection:

Gene Information

Myo10
Name: myosin X
Synonyms: D15Ertd600e, myosin-X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Gga3
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 3
Synonyms: C230037M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 260302
Homologene: 121703
Notch3
Name: notch 3
Synonyms: hpbk, N3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Igf1
Name: insulin-like growth factor 1
Synonyms: C730016P09Rik, Igf-I, Igf-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16000
HGNC: HGNC:5464
Homologene: 515
Dlc1
Name: deleted in liver cancer 1
Synonyms: STARD12, Arhgap7, p122-RhoGAP, A730069N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: D230004K18Rik, b2b1625.2Clo, Gp330, Megalin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Shroom3
Name: shroom family member 3
Synonyms: Shrm3, D5Ertd287e, Shrm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Dhcr24
Name: 24-dehydrocholesterol reductase
Synonyms: 2310076D10Rik, 3-beta-hydroxysterol delta-24 reductase, seladin-1, 5830417J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74754
HGNC: HGNC:2859
Homologene: 8850
Sacm1l
Name: SAC1 suppressor of actin mutations 1-like (yeast)
Synonyms: SAC1, Sac1p
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 83493
VEGA: 9
Homologene: 6320
Ttc17
Name: tetratricopeptide repeat domain 17
Synonyms: D2Bwg1005e, 9130020K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74569
Homologene: 10100
Nol6
Name: nucleolar protein family 6 (RNA-associated)
Synonyms: Nrap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230082
Homologene: 41505
Ccdc34
Name: coiled-coil domain containing 34
Synonyms: 4930522P10Rik, 2810027O19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68201
Homologene: 12245
Calcr
Name: calcitonin receptor
Synonyms: Clr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12311
HGNC: HGNC:1440
Homologene: 1320
Ntn1
Name: netrin 1
Synonyms: Netrin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18208
HGNC: HGNC:8029
Homologene: 21008
Lrrc8a
Name: leucine rich repeat containing 8A VRAC subunit A
Synonyms: ebo, SWELL1, Lrrc8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241296
Homologene: 18617
Ms4a4c
Name: membrane-spanning 4-domains, subfamily A, member 4C
Synonyms: 5830413L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 64380
Homologene: 113790
Lifr
Name: LIF receptor alpha
Synonyms: A230075M04Rik, soluble differentiation-stimulating factor receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16880
VEGA: 15
HGNC: HGNC:6597
Homologene: 1735
Abcf3
Name: ATP-binding cassette, sub-family F (GCN20), member 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 27406
HGNC: HGNC:72
Homologene: 22784
Golga2
Name: golgi autoantigen, golgin subfamily a, 2
Synonyms: GM130
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99412
HGNC: HGNC:4425
Homologene: 3300
Itpkc
Name: inositol 1,4,5-trisphosphate 3-kinase C
Synonyms: 9130023N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233011
Homologene: 11868
Tnrc6c
Name: trinucleotide repeat containing 6C
Synonyms: 9930033H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217351
Homologene: 23137
Pcdh17
Name: protocadherin 17
Synonyms: C030033F14Rik, LOC219228
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219228
VEGA: 14
Homologene: 8700
Ttn
Name: titin
Synonyms: L56, 2310074I15Rik, 2310036G12Rik, 1100001C23Rik, connectin, shru, D830007G01Rik, mdm, D330041I19Rik, 2310057K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100213
Homologene: 18967
Klhl1
Name: kelch-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 93688
HGNC: HGNC:6352
Homologene: 56903
Svs1
Name: seminal vesicle secretory protein 1
Synonyms: SVS I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243377
Homologene: 18250
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: CRMP-3, DPY4, Drp-4, Crmp3, Ulip4, unc-33-like phosphoprotein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Pik3r2
Name: phosphoinositide-3-kinase regulatory subunit 2
Synonyms: p85beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18709
HGNC: HGNC:8980
Homologene: 3687
Trim43a
Name: tripartite motif-containing 43A
Synonyms: Gm6021
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 547109
Homologene: 105432
C330019G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Dpp10
Name: dipeptidylpeptidase 10
Synonyms: 6430601K09Rik, DPRP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269109
Homologene: 41400
Csmd2
Name: CUB and Sushi multiple domains 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329942
Homologene: 89034
Vmn2r9
Name: vomeronasal 2, receptor 9
Synonyms: EG435864
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 435864
Homologene: 129606
AY358078
Name: cDNA sequence AY358078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 278676
Homologene: 115686
Vmn1r60
Name: vomeronasal 1 receptor 60
Synonyms: Gm7184
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 636697
Homologene: 41799
Tas2r104
Name: taste receptor, type 2, member 104
Synonyms: T2R04, Tas2r4, mGR04, mt2r45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387340
Homologene: 130072
Abhd4
Name: abhydrolase domain containing 4
Synonyms: 1110035H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105501
Homologene: 56937
Mios
Name: meiosis regulator for oocyte development
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 252875
Homologene: 10371
Mstn
Name: myostatin
Synonyms: Gdf8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17700
HGNC: HGNC:4223
Homologene: 3850
Mrps11
Name: mitochondrial ribosomal protein S11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67994
Homologene: 32554
Olfr585
Name: olfactory receptor 585
Synonyms: MOR14-4, GA_x6K02T2PBJ9-5809085-5810035
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259091
Homologene: 133721
Gm572
Name: predicted gene 572
Synonyms: LOC230909, b2b1167Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230909
Homologene: 52134
AI429214
Name: expressed sequence AI429214
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 621080
Homologene: 82747
Dusp7
Name: dual specificity phosphatase 7
Synonyms: MKP-X, PYST2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235584
HGNC: HGNC:3073
Homologene: 1468
Olfr547
Name: olfactory receptor 547
Synonyms: GA_x6K02T2PBJ9-5256044-5256988, MOR31-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259083
Homologene: 17493
Slco4a1
Name: solute carrier organic anion transporter family, member 4a1
Synonyms: Slc21a12, OATP-E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108115
Homologene: 9482
Gm10271
Name: predicted gene 10271
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,061,971 bp
  • A to T, chromosome 1 at 123,485,924 bp
  • C to T, chromosome 2 at 30,261,960 bp
  • A to G, chromosome 2 at 32,305,611 bp
  • A to G, chromosome 2 at 69,442,734 bp
  • A to G, chromosome 2 at 69,537,565 bp
  • G to T, chromosome 2 at 76,751,334 bp
  • A to C, chromosome 2 at 76,955,024 bp
  • A to T, chromosome 2 at 94,369,413 bp
  • A to G, chromosome 2 at 110,032,428 bp
  • C to T, chromosome 2 at 180,464,091 bp
  • A to G, chromosome 4 at 41,117,452 bp
  • C to A, chromosome 4 at 43,416,424 bp
  • A to G, chromosome 4 at 106,573,873 bp
  • G to A, chromosome 4 at 128,338,711 bp
  • G to A, chromosome 4 at 148,668,872 bp
  • T to C, chromosome 5 at 32,830,345 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • A to G, chromosome 5 at 108,848,131 bp
  • T to C, chromosome 6 at 3,693,735 bp
  • T to A, chromosome 6 at 8,216,453 bp
  • C to T, chromosome 6 at 48,987,994 bp
  • A to G, chromosome 6 at 73,127,927 bp
  • A to T, chromosome 6 at 131,684,925 bp
  • A to T, chromosome 7 at 5,545,027 bp
  • A to T, chromosome 7 at 27,227,612 bp
  • T to C, chromosome 7 at 78,790,645 bp
  • C to A, chromosome 7 at 102,535,280 bp
  • T to C, chromosome 7 at 103,098,184 bp
  • C to T, chromosome 7 at 139,100,935 bp
  • A to G, chromosome 8 at 36,763,633 bp
  • A to G, chromosome 8 at 36,994,442 bp
  • G to A, chromosome 8 at 70,770,421 bp
  • T to A, chromosome 9 at 88,840,401 bp
  • T to A, chromosome 9 at 106,373,893 bp
  • T to A, chromosome 9 at 123,587,576 bp
  • A to T, chromosome 10 at 87,915,319 bp
  • T to A, chromosome 10 at 116,956,874 bp
  • C to G, chromosome 11 at 68,385,793 bp
  • T to A, chromosome 11 at 115,587,542 bp
  • C to T, chromosome 11 at 117,723,529 bp
  • A to G, chromosome 14 at 51,805,553 bp
  • T to C, chromosome 14 at 54,265,367 bp
  • C to T, chromosome 14 at 84,447,259 bp
  • A to T, chromosome 14 at 96,280,205 bp
  • T to A, chromosome 15 at 7,186,853 bp
  • A to G, chromosome 15 at 25,779,626 bp
  • T to C, chromosome 16 at 20,560,439 bp
  • A to G, chromosome 17 at 32,135,556 bp
  • T to A, chromosome 19 at 11,416,395 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3852 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040784-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.