Strain Name:
C57BL/6J-MtgxR4001Btlr/Mmmh
Stock Number:
040844-MU
Citation ID:
RRID:MMRRC_040844-MU
Other Names:
R4001 (G1), C57BL/6J-MtgxR4001Btlr
Major Collection:

Strain Information

Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 27223
Homologene: 4137
Senp2
Name: SUMO/sentrin specific peptidase 2
Synonyms: 4930538C18Rik, 2310007L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 75826
VEGA: 16
Homologene: 11005
Uaca
Name: uveal autoantigen with coiled-coil domains and ankyrin repeats
Synonyms: nucling, 2700059D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72565
VEGA: 9
Homologene: 74297
Chd9
Name: chromodomain helicase DNA binding protein 9
Synonyms: 1810014J18Rik, AD013, A330063D19Rik, 9030205D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 109151
Homologene: 11844
Clptm1l
Name: CLPTM1-like
Synonyms: C130052I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218335
VEGA: 13
Homologene: 12767
Naa16
Name: N(alpha)-acetyltransferase 16, NatA auxiliary subunit
Synonyms: 1300019C06Rik, Narg1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66897
Homologene: 134838
Rbms2
Name: RNA binding motif, single stranded interacting protein 2
Synonyms: Scr3, 2610315E04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56516
VEGA: 10
HGNC: HGNC:9909
Homologene: 37706
Aars
Name: alanyl-tRNA synthetase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234734
HGNC: HGNC:20
Homologene: 1213
D6Wsu163e
Name: DNA segment, Chr 6, Wayne State University 163, expressed
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 28040
HGNC: HGNC:1184
Homologene: 10685
Tmprss3
Name: transmembrane protease, serine 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 140765
Homologene: 56985
Srcap
Name: Snf2-related CREBBP activator protein
Synonyms: F630004O05Rik, B930091H02Rik, D030022P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043597
Homologene: 38213
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Stradb
Name: STE20-related kinase adaptor beta
Synonyms: PRO1038, D1Ucla2, Als2cr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227154
Homologene: 10237
Cntnap5c
Name: contactin associated protein-like 5C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 620292
VEGA: 17
Homologene: 128806
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Nalcn
Name: sodium leak channel, non-selective
Synonyms: A530023G15Rik, Vgcnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 338370
VEGA: 14
Homologene: 21832
Tro
Name: trophinin
Synonyms: necdin and trophinin like, Tnn, trophinin-2, magphinin-gamma, magphinin-beta 2, magphinin-alpha, magphinin, Maged3l, Maged3, Trol
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 56191
Homologene: 75113
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Cdhr2
Name: cadherin-related family member 2
Synonyms: LOC268663, Pcdh24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268663
Homologene: 134510
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Klhdc7b
Name: kelch domain containing 7B
Synonyms: EG546648
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 546648
Homologene: 34707
Lpcat4
Name: lysophosphatidylcholine acyltransferase 4
Synonyms: Aytl3, Agpat7, LPEAT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99010
Homologene: 27544
Tmc3
Name: transmembrane channel-like gene family 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233424
Homologene: 45588
Rgsl1
Name: regulator of G-protein signaling like 1
Synonyms: 4930415K13Rik, Rgsl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240816
Homologene: 129962
Rasgrf1
Name: RAS protein-specific guanine nucleotide-releasing factor 1
Synonyms: CDC25Mm, Grfbeta, CDC25, Grf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 19417
HGNC: HGNC:9875
Homologene: 74972
Pdss2
Name: prenyl (solanesyl) diphosphate synthase, subunit 2
Synonyms: kd, 5430420P03Rik, mDLP1, PLMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71365
Homologene: 56885
Or1e23
Name: olfactory receptor family 1 subfamily E member 23
Synonyms: GA_x6K02T2P1NL-3676608-3675670, MOR135-14, MOR135-31_p, Olfr382
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258435
Homologene: 17252
Clip4
Name: CAP-GLY domain containing linker protein family, member 4
Synonyms: 1700074B05Rik, 5830409B12Rik, 4833417L20Rik, 1700024K14Rik, Rsnl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78785
VEGA: 17
Homologene: 11662
4930595M18Rik
Name: RIKEN cDNA 4930595M18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245492
Homologene: 138416
Aipl1
Name: aryl hydrocarbon receptor-interacting protein-like 1
Synonyms: A930007I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 114230
HGNC: HGNC:359
Homologene: 22806
Sap18b
Name: Sin3-associated polypeptide 18B
Synonyms: Gm10094
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100041953
Homologene: 4289
4930415L06Rik
Name: RIKEN cDNA 4930415L06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245511
Homologene: 133109
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 58,980,028 bp
  • A to G, chromosome 1 at 153,817,584 bp
  • A to T, chromosome 2 at 112,239,951 bp
  • A to G, chromosome 2 at 121,205,085 bp
  • A to G, chromosome 2 at 125,477,495 bp
  • A to G, chromosome 6 at 126,950,170 bp
  • T to A, chromosome 7 at 81,317,356 bp
  • T to C, chromosome 7 at 83,620,063 bp
  • T to C, chromosome 7 at 127,532,167 bp
  • C to T, chromosome 8 at 90,956,557 bp
  • T to C, chromosome 8 at 95,825,440 bp
  • C to A, chromosome 8 at 111,041,602 bp
  • G to A, chromosome 9 at 60,871,084 bp
  • T to C, chromosome 9 at 89,974,756 bp
  • A to G, chromosome 10 at 14,127,732 bp
  • T to C, chromosome 10 at 43,298,784 bp
  • C to T, chromosome 10 at 128,151,300 bp
  • C to G, chromosome 11 at 59,134,569 bp
  • T to A, chromosome 11 at 72,031,602 bp
  • A to G, chromosome 11 at 73,516,986 bp
  • A to T, chromosome 13 at 54,718,266 bp
  • T to C, chromosome 13 at 73,615,162 bp
  • C to A, chromosome 14 at 79,343,121 bp
  • G to T, chromosome 14 at 123,596,594 bp
  • A to G, chromosome 15 at 89,387,984 bp
  • T to C, chromosome 16 at 22,028,568 bp
  • T to C, chromosome 17 at 31,186,559 bp
  • G to A, chromosome 17 at 58,407,740 bp
  • A to T, chromosome 17 at 71,799,076 bp
  • A to G, chromosome X at 81,421,113 bp
  • T to C, chromosome X at 89,930,510 bp
  • G to A, chromosome X at 150,655,202 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4001 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040844-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.