Strain Name:
C57BL/6J-MtgxR4016Btlr/Mmmh
Stock Number:
040847-MU
Citation ID:
RRID:MMRRC_040847-MU
Other Names:
R4016 (G1), C57BL/6J-MtgxR4016Btlr
Major Collection:

Gene Information

Tyr
Name: tyrosinase
Synonyms: Oca1, skc35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22173
Homologene: 30969
Ntrk3
Name: neurotrophic tyrosine kinase, receptor, type 3
Synonyms: TrkC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18213
HGNC: HGNC:8033
Homologene: 49183
Ptch1
Name: patched 1
Synonyms: Ptc1, Patched 1, A230106A15Rik, wig, Ptc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Unc93b1
Name: unc-93 homolog B1, TLR signaling regulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 54445
Homologene: 41325
Igf2bp2
Name: insulin-like growth factor 2 mRNA binding protein 2
Synonyms: IMP2, C330012H03Rik, IMP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 319765
Homologene: 4774
Ezh2
Name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Synonyms: KMT6, Enx1h, Enx-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 14056
HGNC: HGNC:3527
Homologene: 37926
Kdm5a
Name: lysine (K)-specific demethylase 5A
Synonyms: Rbbp2, Jarid1a, RBP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 214899
HGNC: HGNC:9886
Homologene: 3419
Rps27a
Name: ribosomal protein S27A
Synonyms: 0610006J14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 78294
Homologene: 37715
Tox4
Name: TOX high mobility group box family member 4
Synonyms: 5730589K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 268741
Homologene: 8889
Zfp566
Name: zinc finger protein 566
Synonyms: 2700043M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72556
Homologene: 88470
Dlat
Name: dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Synonyms: 6332404G05Rik, PDC-E2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235339
HGNC: HGNC:2896
Homologene: 6814
Stra6
Name: stimulated by retinoic acid gene 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20897
Homologene: 7554
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Rbp-3, Irbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Umod
Name: uromodulin
Synonyms: Tamm-Horsfall glycoprotein, uromucoid, Urehd1, urehr4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 22242
Homologene: 2522
Capn11
Name: calpain 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 268958
HGNC: HGNC:1478
Homologene: 21392
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: ecNOS, eNOS, 2310065A03Rik, Nos-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: 3110001A05Rik, 3110027P15Rik, 1810064L21Rik, Ugcgl2, A230065J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 66435
Homologene: 56875
Nlrp1b
Name: NLR family, pyrin domain containing 1B
Synonyms: Nalp1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 637515
Homologene: 19080
Dock10
Name: dedicator of cytokinesis 10
Synonyms: Zizimin3, Jr4, Jr5, A630054M16Rik, 9330153B10Rik, ZIZ3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 210293
Homologene: 45952
Gm6614
Name: predicted gene 6614
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 625716
Homologene: 87075
Whrn
Name: whirlin
Synonyms: Dfnb31, Ush2d, C430046P22Rik, wi, 1110035G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 73750
Homologene: 18739
Aldh8a1
Name: aldehyde dehydrogenase 8 family, member A1
Synonyms: RALDH4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237320
Homologene: 23369
Ptar1
Name: protein prenyltransferase alpha subunit repeat containing 1
Synonyms: 2010013L17Rik, 4930428J16Rik, 1700084D09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 72351
VEGA: 19
Homologene: 34254
Tex14
Name: testis expressed gene 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 83560
Homologene: 12838
Olfr1474
Name: olfactory receptor 1474
Synonyms: GA_x6K02T2RE5P-3803583-3804527, MOR202-26P, MOR202-42
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258123
HGNC: null
Homologene: 79417
Smarca2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms: brm, Snf2l2, 2610209L14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 67155
Homologene: 2308
Vmn2r99
Name: vomeronasal 2, receptor 99
Synonyms: EG665376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 665376
HGNC: null
Homologene: 115024
P2rx1
Name: purinergic receptor P2X, ligand-gated ion channel, 1
Synonyms: RP-2, P2X1 receptor, P2x, Pdcd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18436
HGNC: HGNC:8533
Homologene: 1921
Map3k21
Name: mitogen-activated protein kinase kinase kinase 21
Synonyms: BC021891
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234878
Homologene: 32778
Pdzrn4
Name: PDZ domain containing RING finger 4
Synonyms: SAMCAP3L, LNX4, 1110017D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239618
VEGA: 15
Homologene: 85433
Dtx3
Name: deltex 3, E3 ubiquitin ligase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 80904
Homologene: 12754
Scnn1b
Name: sodium channel, nonvoltage-gated 1 beta
Synonyms: ENaC beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20277
Homologene: 133555
Slc16a14
Name: solute carrier family 16 (monocarboxylic acid transporters), member 14
Synonyms: 1110004H10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 71781
Homologene: 34318
Ano3
Name: anoctamin 3
Synonyms: Tmem16c, B230324K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228432
Homologene: 57147
Apold1
Name: apolipoprotein L domain containing 1
Synonyms: LOC381823
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 381823
Homologene: 12781
Spatc1l
Name: spermatogenesis and centriole associated 1 like
Synonyms: 1700022B01Rik, 1700027D21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 76573
HGNC: HGNC:1298
Homologene: 75292
Nipal2
Name: NIPA-like domain containing 2
Synonyms: Npal2, 9330161F08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223473
VEGA: 15
Homologene: 41586
Dctpp1
Name: dCTP pyrophosphatase 1
Synonyms: 2410015N17Rik, RS21-C6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 66422
Homologene: 11329
Sgms1
Name: sphingomyelin synthase 1
Synonyms: SMS1alpha2, 9530058O11Rik, SMS1, Tmem23, SMS1beta, SMS1alpha1, SMS1gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 208449
Homologene: 27040
Purg
Name: purine-rich element binding protein G
Synonyms: 4930486B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 75029
Homologene: 22747
Mrgpra6
Name: MAS-related GPR, member A6
Synonyms: MrgA6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 381886
HGNC: null
Homologene: null
Gm1979
Name: predicted gene 1979
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100038949
HGNC: null
Homologene: 69402
Arpc5
Name: actin related protein 2/3 complex, subunit 5
Synonyms: p16-Arc, 5830443F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 67771
HGNC: HGNC:708
Homologene: 4176
Svip
Name: small VCP/p97-interacting protein
Synonyms: 6620401M08Rik, 1700006C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 75744
Homologene: 85776
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 80,606,569 bp
  • G to T, chromosome 1 at 84,912,507 bp
  • G to A, chromosome 1 at 152,768,856 bp
  • A to G, chromosome 2 at 110,774,976 bp
  • G to A, chromosome 4 at 63,415,639 bp
  • G to A, chromosome 5 at 24,371,716 bp
  • C to T, chromosome 5 at 26,004,606 bp
  • A to G, chromosome 6 at 47,544,582 bp
  • T to A, chromosome 6 at 120,394,106 bp
  • A to G, chromosome 6 at 134,983,906 bp
  • G to T, chromosome 6 at 141,972,429 bp
  • A to G, chromosome 7 at 30,081,081 bp
  • C to A, chromosome 7 at 47,188,715 bp
  • A to T, chromosome 7 at 52,003,994 bp
  • G to T, chromosome 7 at 78,462,947 bp
  • A to G, chromosome 7 at 87,437,940 bp
  • A to C, chromosome 7 at 119,476,690 bp
  • G to T, chromosome 7 at 121,914,332 bp
  • G to A, chromosome 7 at 127,257,113 bp
  • T to A, chromosome 8 at 33,386,991 bp
  • T to A, chromosome 8 at 125,911,185 bp
  • C to T, chromosome 9 at 50,649,631 bp
  • T to A, chromosome 9 at 58,135,190 bp
  • G to A, chromosome 10 at 21,395,571 bp
  • A to G, chromosome 10 at 76,562,489 bp
  • A to G, chromosome 10 at 127,191,171 bp
  • A to G, chromosome 11 at 29,546,433 bp
  • A to G, chromosome 11 at 71,173,085 bp
  • T to C, chromosome 11 at 73,009,973 bp
  • A to G, chromosome 11 at 87,538,623 bp
  • T to G, chromosome 13 at 63,524,959 bp
  • G to T, chromosome 14 at 33,955,390 bp
  • T to C, chromosome 14 at 52,285,904 bp
  • T to C, chromosome 14 at 119,026,433 bp
  • T to G, chromosome 15 at 34,600,061 bp
  • T to A, chromosome 15 at 92,399,749 bp
  • T to C, chromosome 16 at 22,063,676 bp
  • C to T, chromosome 17 at 19,378,570 bp
  • T to A, chromosome 17 at 45,653,756 bp
  • T to C, chromosome 19 at 3,943,572 bp
  • A to T, chromosome 19 at 13,471,197 bp
  • A to G, chromosome 19 at 23,687,460 bp
  • G to A, chromosome 19 at 26,683,927 bp
  • A to G, chromosome 19 at 32,142,792 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4016 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040847-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.