Strain Name:
C57BL/6J-MtgxR4127Btlr/Mmmh
Stock Number:
040860-MU
Citation ID:
RRID:MMRRC_040860-MU
Other Names:
R4127 (G1), C57BL/6J-MtgxR4127Btlr
Major Collection:

Gene Information

Pias3
Name: protein inhibitor of activated STAT 3
Synonyms: Pias3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 229615
Homologene: 4447
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 6030440P17Rik, ne, 8430406N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242022
Homologene: 18454
Jarid2
Name: jumonji, AT rich interactive domain 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Pxn
Name: paxillin
Synonyms: Pax
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19303
HGNC: HGNC:9718
Homologene: 37697
Zranb2
Name: zinc finger, RAN-binding domain containing 2
Synonyms: Zfp265, Znf265, Zis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 53861
Etl4
Name: enhancer trap locus 4
Synonyms: Sickle tail, 6620402G01Rik, 9430077C05Rik, Etl-4, Skt, E330027G05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 208618
Homologene: 10477
Gtf3c1
Name: general transcription factor III C 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233863
HGNC: HGNC:4664
Homologene: 31040
Tbc1d8
Name: TBC1 domain family, member 8
Synonyms: AD3, GRAM domain, BUB2-like protein 1, HBLP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 54610
Homologene: 31421
Tep1
Name: telomerase associated protein 1
Synonyms: Tp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 21745
VEGA: 14
Homologene: 5157
Cyfip2
Name: cytoplasmic FMR1 interacting protein 2
Synonyms: Pir121, 6430511D02Rik, 1500004I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 76884
Homologene: 7936
Scp2
Name: sterol carrier protein 2, liver
Synonyms: nonspecific lipid transfer protein, ns-LTP, SCP-2, SCPx, NSL-TP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 20280
Homologene: 37717
Polg
Name: polymerase (DNA directed), gamma
Synonyms: polymerase gamma, Pol gamma, Polga, mitochondrial DNA polymerase gamma, mitochondrial DNA polymerase-gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18975
HGNC: HGNC:9179
Homologene: 2016
Rag1
Name: recombination activating 1
Synonyms: Rag-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 19373
HGNC: HGNC:9831
Homologene: 387
Pus10
Name: pseudouridylate synthase 10
Synonyms: Ccdc139, 2810013G11Rik, 4933435A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74467
Homologene: 12566
Chn2
Name: chimerin 2
Synonyms: 4930557O16Rik, 1700026N20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Fchsd1
Name: FCH and double SH3 domains 1
Synonyms: A030002D08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 319262
Homologene: 14127
Gga2
Name: golgi associated, gamma adaptin ear containing, ARF binding protein 2
Synonyms: 1200007E24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74105
Homologene: 22860
Heatr5b
Name: HEAT repeat containing 5B
Synonyms: D330050P16Rik, A230048G03Rik, 2010013B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 320473
VEGA: 17
Homologene: 25536
Stra6
Name: stimulated by retinoic acid gene 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 20897
Homologene: 7554
Cd209b
Name: CD209b antigen
Synonyms: mSIGNR1, 1810030I22Rik, SIGNR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 69165
HGNC: HGNC:1641
Homologene: 84858
Abca13
Name: ATP-binding cassette, sub-family A (ABC1), member 13
Synonyms: A930002G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 268379
Homologene: 27991
Sorcs1
Name: sortilin-related VPS10 domain containing receptor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 58178
Homologene: 10967
Slc9b2
Name: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Synonyms: NHA2, Nhedc2, C80638, NHE10, nha-oc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 97086
Homologene: 45381
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mgr1, Mass1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 110789
Homologene: 19815
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: E130113P14Rik, bl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231470
Homologene: 23516
Xcr1
Name: chemokine (C motif) receptor 1
Synonyms: Ccxcr1, C motif-1/lymphotactin receptor, GPR5, XCR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 23832
HGNC: HGNC:1625
Homologene: 21095
Cgnl1
Name: cingulin-like 1
Synonyms: Jacop, 4933421H10Rik, 9930020M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 68178
Homologene: 41901
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 435626
Homologene: 52359
Tmem132d
Name: transmembrane protein 132D
Synonyms: C630028F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 243274
Homologene: 71684
Pcdhb2
Name: protocadherin beta 2
Synonyms: PcdhbB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93873
HGNC: HGNC:8687
Homologene: 69266
Actg2
Name: actin, gamma 2, smooth muscle, enteric
Synonyms: Act4, SMGA, Acta3, Act-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11468
HGNC: HGNC:145
Homologene: 123845
Ankrd6
Name: ankyrin repeat domain 6
Synonyms: diversin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 140577
Homologene: 8967
Atp6ap1l
Name: ATPase, H+ transporting, lysosomal accessory protein 1-like
Synonyms: EG435376
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 435376
Homologene: 28130
Gm9892
Name: predicted gene 9892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 100040220
Gm5592
Name: predicted gene 5592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 434172
Homologene: 45597
Ubash3a
Name: ubiquitin associated and SH3 domain containing, A
Synonyms: Sts-2, 5830413C03Rik, TULA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328795
Homologene: 56833
Olfr1163
Name: olfactory receptor 1163
Synonyms: MOR174-8, GA_x6K02T2Q125-49563265-49562315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258638
Homologene: 17328
Lzts3
Name: leucine zipper, putative tumor suppressor family member 3
Synonyms: Prosapip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241638
Homologene: 8824
Gm9988
Name: predicted gene 9988
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Cfl2
Name: cofilin 2, muscle
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 12632
VEGA: 12
HGNC: HGNC:1875
Homologene: 129115
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 39,372,431 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 2 at 20,744,075 bp
  • A to G, chromosome 2 at 88,071,235 bp
  • A to G, chromosome 2 at 101,642,071 bp
  • A to G, chromosome 2 at 130,635,365 bp
  • T to C, chromosome 3 at 53,525,896 bp
  • G to T, chromosome 3 at 96,699,666 bp
  • T to C, chromosome 3 at 135,329,837 bp
  • C to A, chromosome 3 at 157,537,590 bp
  • G to A, chromosome 4 at 32,822,241 bp
  • A to G, chromosome 4 at 108,063,984 bp
  • A to G, chromosome 5 at 96,770,653 bp
  • A to G, chromosome 5 at 115,546,907 bp
  • T to C, chromosome 5 at 128,268,820 bp
  • G to T, chromosome 6 at 54,272,978 bp
  • A to T, chromosome 6 at 83,522,884 bp
  • G to A, chromosome 7 at 41,289,067 bp
  • T to C, chromosome 7 at 79,455,537 bp
  • G to T, chromosome 7 at 122,002,720 bp
  • A to T, chromosome 7 at 125,647,450 bp
  • A to G, chromosome 8 at 3,918,714 bp
  • A to G, chromosome 8 at 52,196,263 bp
  • T to A, chromosome 8 at 88,138,311 bp
  • T to A, chromosome 9 at 58,151,218 bp
  • T to C, chromosome 9 at 71,724,540 bp
  • A to C, chromosome 9 at 123,856,496 bp
  • A to G, chromosome 11 at 9,191,973 bp
  • T to C, chromosome 11 at 23,718,654 bp
  • T to C, chromosome 11 at 46,270,647 bp
  • C to T, chromosome 12 at 54,861,358 bp
  • T to C, chromosome 13 at 44,902,256 bp
  • C to G, chromosome 13 at 81,528,924 bp
  • T to C, chromosome 13 at 90,898,707 bp
  • C to T, chromosome 14 at 50,843,734 bp
  • T to C, chromosome 17 at 31,237,275 bp
  • A to G, chromosome 17 at 78,753,174 bp
  • A to T, chromosome 18 at 37,295,541 bp
  • A to G, chromosome 18 at 37,958,214 bp
  • T to C, chromosome 19 at 50,222,159 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4127 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040860-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.