Strain Name:
C57BL/6J-MtgxR1521Btlr/Mmmh
Stock Number:
040870-MU
Citation ID:
RRID:MMRRC_040870-MU
Other Names:
R1521 (G1), C57BL/6J-MtgxR1521Btlr
Major Collection:

Gene Information

Gene Symbol: Pde1c [MGI:108413] (Mus musculus (mouse))
Name: phosphodiesterase 1C
Chromosome: 6
Alteration at locus: Chemically Induced
Gene Symbol: Cacna1h [MGI:1928842] (Mus musculus (mouse))
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Chromosome: 17
Alteration at locus: Chemically Induced
Gene Symbol: Eya1 [MGI:109344] (Mus musculus (mouse))
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Chromosome: 1
Alteration at locus: Chemically Induced
Gene Symbol: Tert [MGI:1202709] (Mus musculus (mouse))
Name: telomerase reverse transcriptase
Synonyms: TR
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Map1b [MGI:1306778] (Mus musculus (mouse))
Name: microtubule-associated protein 1B
Synonyms: Mtap5, Mtap1b, Mtap-5, MAP5, LC1
Chromosome: 13
Alteration at locus: Chemically Induced
Gene Symbol: Odf2l [MGI:1098600] (Mus musculus (mouse))
Name: outer dense fiber of sperm tails 2-like
Synonyms: 9630045K08Rik, 4733401D09Rik
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Tex21 [MGI:1931131] (Mus musculus (mouse))
Name: testis expressed gene 21
Synonyms: 4931406F04Rik, 4931421K24Rik, 4931412D23Rik, tsec-2
Chromosome: 12
Alteration at locus: Chemically Induced
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 14,274,550 bp
  • C to A, chromosome 1 at 36,068,886 bp
  • T to C, chromosome 1 at 52,090,859 bp
  • T to C, chromosome 1 at 106,392,319 bp
  • A to G, chromosome 1 at 131,750,677 bp
  • A to C, chromosome 1 at 143,798,422 bp
  • T to C, chromosome 1 at 149,857,686 bp
  • G to A, chromosome 1 at 151,351,558 bp
  • C to T, chromosome 1 at 153,166,263 bp
  • T to A, chromosome 1 at 187,193,994 bp
  • T to A, chromosome 1 at 191,164,022 bp
  • T to C, chromosome 2 at 10,106,747 bp
  • G to T, chromosome 2 at 14,614,352 bp
  • G to A, chromosome 2 at 54,854,645 bp
  • T to C, chromosome 2 at 76,741,167 bp
  • G to A, chromosome 2 at 89,835,172 bp
  • T to C, chromosome 2 at 106,351,669 bp
  • T to A, chromosome 2 at 129,304,741 bp
  • A to C, chromosome 2 at 155,415,222 bp
  • A to G, chromosome 2 at 163,645,539 bp
  • G to A, chromosome 2 at 179,797,558 bp
  • G to T, chromosome 3 at 30,936,575 bp
  • T to C, chromosome 3 at 90,257,383 bp
  • T to A, chromosome 3 at 90,591,292 bp
  • T to A, chromosome 3 at 100,194,615 bp
  • T to G, chromosome 3 at 103,777,782 bp
  • T to C, chromosome 3 at 145,149,036 bp
  • T to C, chromosome 4 at 11,527,894 bp
  • A to G, chromosome 4 at 32,631,867 bp
  • T to C, chromosome 4 at 32,996,005 bp
  • C to T, chromosome 4 at 61,835,017 bp
  • A to G, chromosome 4 at 88,721,993 bp
  • A to T, chromosome 4 at 118,432,000 bp
  • T to C, chromosome 4 at 134,251,350 bp
  • T to C, chromosome 4 at 140,515,438 bp
  • G to T, chromosome 4 at 145,105,861 bp
  • T to C, chromosome 4 at 155,650,717 bp
  • A to G, chromosome 5 at 22,227,519 bp
  • T to A, chromosome 5 at 24,149,110 bp
  • T to C, chromosome 5 at 24,422,323 bp
  • G to A, chromosome 5 at 33,413,934 bp
  • A to C, chromosome 5 at 121,779,591 bp
  • G to A, chromosome 5 at 129,595,088 bp
  • C to A, chromosome 6 at 22,145,512 bp
  • T to A, chromosome 6 at 30,376,025 bp
  • A to G, chromosome 6 at 56,173,607 bp
  • T to C, chromosome 6 at 81,923,812 bp
  • G to T, chromosome 6 at 89,212,609 bp
  • A to G, chromosome 7 at 24,125,785 bp
  • C to T, chromosome 7 at 28,075,160 bp
  • A to G, chromosome 7 at 43,782,880 bp
  • T to C, chromosome 7 at 43,829,275 bp
  • A to T, chromosome 7 at 46,259,264 bp
  • T to A, chromosome 7 at 103,173,451 bp
  • T to A, chromosome 7 at 112,381,610 bp
  • C to T, chromosome 7 at 121,930,574 bp
  • G to T, chromosome 7 at 127,027,839 bp
  • C to T, chromosome 7 at 127,867,080 bp
  • A to T, chromosome 7 at 142,515,825 bp
  • C to T, chromosome 7 at 143,082,889 bp
  • A to T, chromosome 8 at 22,027,673 bp
  • G to A, chromosome 8 at 84,726,526 bp
  • A to T, chromosome 8 at 84,965,858 bp
  • A to G, chromosome 8 at 111,987,235 bp
  • T to C, chromosome 8 at 117,065,500 bp
  • A to G, chromosome 9 at 18,456,563 bp
  • C to T, chromosome 9 at 19,338,652 bp
  • A to C, chromosome 9 at 20,260,432 bp
  • A to C, chromosome 9 at 20,348,775 bp
  • A to C, chromosome 9 at 31,108,215 bp
  • T to G, chromosome 9 at 38,657,718 bp
  • A to T, chromosome 9 at 39,550,784 bp
  • A to C, chromosome 9 at 57,242,465 bp
  • A to T, chromosome 9 at 59,949,205 bp
  • A to C, chromosome 9 at 109,007,305 bp
  • A to G, chromosome 9 at 110,849,635 bp
  • T to C, chromosome 10 at 4,354,804 bp
  • A to G, chromosome 10 at 43,998,416 bp
  • A to G, chromosome 10 at 64,959,842 bp
  • T to C, chromosome 10 at 74,594,191 bp
  • A to G, chromosome 10 at 75,859,541 bp
  • T to C, chromosome 10 at 80,835,721 bp
  • T to A, chromosome 10 at 84,667,313 bp
  • T to A, chromosome 10 at 93,847,186 bp
  • A to G, chromosome 10 at 122,090,479 bp
  • A to G, chromosome 10 at 128,957,811 bp
  • A to T, chromosome 10 at 130,389,268 bp
  • T to G, chromosome 10 at 130,425,919 bp
  • T to A, chromosome 11 at 5,876,833 bp
  • T to A, chromosome 11 at 46,610,473 bp
  • C to T, chromosome 11 at 49,636,339 bp
  • T to C, chromosome 11 at 58,859,555 bp
  • T to A, chromosome 11 at 69,988,635 bp
  • C to T, chromosome 11 at 86,776,738 bp
  • C to T, chromosome 11 at 95,835,960 bp
  • T to A, chromosome 11 at 116,684,347 bp
  • T to C, chromosome 12 at 5,091,307 bp
  • T to C, chromosome 12 at 10,394,613 bp
  • A to T, chromosome 12 at 65,121,704 bp
  • A to G, chromosome 12 at 66,568,926 bp
  • A to G, chromosome 12 at 76,204,270 bp
  • C to T, chromosome 12 at 105,809,513 bp
  • T to C, chromosome 12 at 110,554,886 bp
  • T to C, chromosome 12 at 112,036,410 bp
  • T to A, chromosome 13 at 22,557,078 bp
  • A to G, chromosome 13 at 73,642,056 bp
  • T to C, chromosome 13 at 99,432,739 bp
  • T to G, chromosome 13 at 119,703,591 bp
  • A to G, chromosome 14 at 31,262,594 bp
  • T to G, chromosome 14 at 61,005,106 bp
  • A to T, chromosome 15 at 8,333,095 bp
  • T to A, chromosome 15 at 100,618,065 bp
  • T to C, chromosome 16 at 4,996,476 bp
  • T to A, chromosome 16 at 23,494,637 bp
  • C to T, chromosome 16 at 58,760,853 bp
  • G to A, chromosome 16 at 90,753,863 bp
  • T to A, chromosome 17 at 17,971,015 bp
  • A to T, chromosome 17 at 22,145,832 bp
  • T to C, chromosome 17 at 25,397,354 bp
  • G to T, chromosome 17 at 25,788,781 bp
  • T to A, chromosome 17 at 33,033,302 bp
  • T to C, chromosome 17 at 34,711,503 bp
  • T to C, chromosome 17 at 43,430,552 bp
  • T to A, chromosome 17 at 46,663,190 bp
  • T to A, chromosome 17 at 65,484,501 bp
  • T to A, chromosome 17 at 89,910,306 bp
  • A to G, chromosome 18 at 62,008,488 bp
  • A to T, chromosome 19 at 5,948,406 bp
  • G to A, chromosome 19 at 9,004,728 bp
  • A to G, chromosome 19 at 22,901,221 bp
  • A to T, chromosome 19 at 29,811,446 bp
  • A to T, chromosome 19 at 34,587,173 bp
  • A to G, chromosome 19 at 36,094,385 bp
  • C to A, chromosome 19 at 39,643,264 bp
  • T to C, chromosome 19 at 41,321,558 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R1521 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040870-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.