Strain Name:
C57BL/6J-MtgxR3948Btlr/Mmmh
Stock Number:
040928-MU
Citation ID:
RRID:MMRRC_040928-MU
Other Names:
R3948 (G1), C57BL/6J-MtgxR3948Btlr
Major Collection:

Strain Information

Rev1
Name: REV1, DNA directed polymerase
Synonyms: Rev1l, REV1, 1110027I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 56210
Homologene: 32309
Dhx15
Name: DEAH-box helicase 15
Synonyms: DEAH (Asp-Glu-Ala-His) box polypeptide 15, HRH2, DBP1, mDEAH9, Ddx15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13204
HGNC: HGNC:2738
Homologene: 1040
Fbxo38
Name: F-box protein 38
Synonyms: 6030410I24Rik, SP329
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 107035
VEGA: 18
Homologene: 34526
Arfgef1
Name: ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms: D130059B05Rik, P200, BIG1, ARFGEP1, D730028O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211673
Homologene: 4687
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: Tulip1, 2310003F20Rik, 4930400K19Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Iqsec3
Name: IQ motif and Sec7 domain 3
Synonyms: BRAG3, synarfGEF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243621
Homologene: 46091
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Wdr25
Name: WD repeat domain 25
Synonyms: B930090D16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 212198
VEGA: 12
Homologene: 56993
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237860
Homologene: 14116
Avil
Name: advillin
Synonyms: DOC6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11567
Homologene: 38200
Ttn
Name: titin
Synonyms: D330041I19Rik, 2310057K23Rik, connectin, shru, 1100001C23Rik, L56, mdm, D830007G01Rik, 2310074I15Rik, 2310036G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Avl9
Name: AVL9 cell migration associated
Synonyms: D730049P16Rik, 5830411G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 78937
Homologene: 62425
Irak3
Name: interleukin-1 receptor-associated kinase 3
Synonyms: IRAK-M, 4833428C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73914
Homologene: 36215
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Ankrd50
Name: ankyrin repeat domain 50
Synonyms: E430012K20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99696
Homologene: 129859
Hal
Name: histidine ammonia lyase
Synonyms: histidase, Hsd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15109
HGNC: HGNC:4806
Homologene: 68229
Serpinb9c
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9c
Synonyms: NK9, Spi11, 3830421J05Rik, ovalbumin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20707
HGNC: HGNC:8955
Kcnj2
Name: potassium inwardly-rectifying channel, subfamily J, member 2
Synonyms: IRK1, Kir2.1, Kcnf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16518
HGNC: HGNC:6263
Homologene: 20249
H2-T24
Name: histocompatibility 2, T region locus 24
Synonyms: H-2T24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15042
Homologene: 69019
Zfp202
Name: zinc finger protein 202
Synonyms: C130037E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 80902
Homologene: 68317
Slc28a3
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Synonyms: Cnt3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 114304
Homologene: 32533
Trim5
Name: tripartite motif-containing 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 667823
Homologene: 75345
Disc1
Name: disrupted in schizophrenia 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244667
HGNC: HGNC:2888
Homologene: 10257
Or4c122
Name: olfactory receptor family 4 subfamily C member 122
Synonyms: MOR233-1, Olfr1228, GA_x6K02T2Q125-50696209-50695274
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258973
Homologene: 74217
Odr4
Name: odr4 GPCR localization factor homolog
Synonyms: BC003331, 1810053E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226499
Homologene: 41202
Mios
Name: meiosis regulator for oocyte development
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 252875
Homologene: 10371
Acnat1
Name: acyl-coenzyme A amino acid N-acyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230161
Homologene: 28287
Dyrk4
Name: dual-specificity tyrosine phosphorylation regulated kinase 4
Synonyms: Dyrk4b, Dyrk4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101320
HGNC: HGNC:3095
Homologene: 37858
Zfp248
Name: zinc finger protein 248
Synonyms: 2810037F07Rik, E130106N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72720
Homologene: 69325
Slc35e4
Name: solute carrier family 35, member E4
Synonyms: A330108F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103710
Homologene: 37409
Adgrg4
Name: adhesion G protein-coupled receptor G4
Synonyms: LOC236798, Gpr112, PGR17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236798
Homologene: 72131
Krtap13
Name: keratin associated protein 13
Synonyms: 4C32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16699
VEGA: 16
Homologene: 114409
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • CAGAG to CAG, chromosome 1 at 10,142,586 bp
  • A to T, chromosome 1 at 38,074,333 bp
  • A to T, chromosome 1 at 150,388,557 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • A to G, chromosome 2 at 89,248,992 bp
  • A to G, chromosome 3 at 38,482,841 bp
  • A to G, chromosome 4 at 49,447,477 bp
  • A to C, chromosome 4 at 144,331,306 bp
  • G to A, chromosome 4 at 145,141,340 bp
  • T to C, chromosome 5 at 52,161,580 bp
  • A to G, chromosome 6 at 8,215,496 bp
  • T to C, chromosome 6 at 56,728,665 bp
  • TTAAATTCAT to TT, chromosome 6 at 118,430,194 bp
  • A to T, chromosome 6 at 121,387,824 bp
  • A to G, chromosome 6 at 126,885,305 bp
  • G to A, chromosome 7 at 104,266,520 bp
  • A to G, chromosome 8 at 125,088,135 bp
  • C to A, chromosome 9 at 40,208,425 bp
  • T to C, chromosome 10 at 93,489,907 bp
  • T to A, chromosome 10 at 120,170,373 bp
  • A to G, chromosome 10 at 127,014,205 bp
  • A to T, chromosome 11 at 3,912,970 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to G, chromosome 11 at 77,398,256 bp
  • A to G, chromosome 11 at 111,072,655 bp
  • C to A, chromosome 12 at 55,698,767 bp
  • T to A, chromosome 12 at 109,027,282 bp
  • A to C, chromosome 13 at 33,150,094 bp
  • C to T, chromosome 13 at 58,563,010 bp
  • A to G, chromosome 16 at 88,751,069 bp
  • T to C, chromosome 17 at 36,017,372 bp
  • A to G, chromosome 18 at 62,529,544 bp
  • C to T, chromosome X at 56,917,754 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3948 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040928-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.