Strain Name:
C57BL/6J-MtgxR4022Btlr/Mmmh
Stock Number:
040956-MU
Citation ID:
RRID:MMRRC_040956-MU
Other Names:
R4022 (G1), C57BL/6J-MtgxR4022Btlr
Major Collection:

Gene Information

Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Ilrun
Name: inflammation and lipid regulator with UBA-like and NBR1-like domains
Synonyms: D17Wsu92e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224647
Homologene: 32575
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 2010005I16Rik, 1110013E22Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66011
Homologene: 36409
Arfgef2
Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
Synonyms: BIG2, E230011G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99371
Homologene: 111880
Lin7c
Name: lin-7 homolog C, crumbs cell polarity complex component
Synonyms: LIN-7-C, LIN-7C, Lin7c, MALS-3, Veli3, D2Ertd520e, 9130007B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22343
Homologene: 22649
Mat2a
Name: methionine adenosyltransferase II, alpha
Synonyms: D630045P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232087
HGNC: HGNC:6904
Homologene: 38112
Cltc
Name: clathrin, heavy polypeptide (Hc)
Synonyms: CHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67300
HGNC: HGNC:2092
Homologene: 3572
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328329
Homologene: 42094
Luzp1
Name: leucine zipper protein 1
Synonyms: Luzp, 2700072H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269593
Homologene: 11545
Fbxo28
Name: F-box protein 28
Synonyms: 5730505P19Rik, 4833428J17Rik, Fbx28, D1Ertd578e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67948
Homologene: 9050
Gstcd
Name: glutathione S-transferase, C-terminal domain containing
Synonyms: 4933434L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67553
Homologene: 11693
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: argonaute 3, eIF2C3, C130014L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214150
Homologene: 49799
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2810030C21Rik, 2700066K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Ddias
Name: DNA damage-induced apoptosis suppressor
Synonyms: noxin, 4632434I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74041
Homologene: 51655
Tsen2
Name: tRNA splicing endonuclease subunit 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381802
Homologene: 41622
Rnf17
Name: ring finger protein 17
Synonyms: MMIP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 30054
VEGA: 14
Homologene: 23727
Adcy4
Name: adenylate cyclase 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 104110
VEGA: 14
HGNC: HGNC:235
Homologene: 23149
Prune2
Name: prune homolog 2
Synonyms: 6330414G02Rik, A330102H22Rik, A230083H22Rik, Olfaxin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 353211
VEGA: 19
Homologene: 18939
Lrrn2
Name: leucine rich repeat protein 2, neuronal
Synonyms: NLRR-2, 5730406J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16980
Homologene: 21274
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Eh domain, SH3 domain regulator of endocytosis 2, Sh3d1B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Dnajb11
Name: DnaJ heat shock protein family (Hsp40) member B11
Synonyms: Dj9, 1810031F23Rik, ABBP-2, ERdj3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67838
Homologene: 9464
Mrnip
Name: MRN complex interacting protein
Synonyms: 3010026O09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68067
Homologene: 75241
Tmem11
Name: transmembrane protein 11
Synonyms: 5730466P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216821
Homologene: 2879
Exoc1
Name: exocyst complex component 1
Synonyms: Sec3p, SEC3, A730011E05Rik, 2810407P21Rik, Sec3l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69940
Homologene: 41241
Uncx
Name: UNC homeobox
Synonyms: Chx4, Uncx4.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22255
Homologene: 56598
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
Sall3
Name: spalt like transcription factor 3
Synonyms: Msal, Msal-1, Spalt, Salt, B130022O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20689
VEGA: 18
Homologene: 18142
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Hps3
Name: HPS3, biogenesis of lysosomal organelles complex 2 subunit 1
Synonyms: Hermansky-Pudlak syndrome 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12807
Homologene: 13019
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20192
Homologene: 68151
Stard9
Name: START domain containing 9
Synonyms: N-3 kinesin, Kif16a, 4831403C07Rik, E230025N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Mroh2a
Name: maestro heat-like repeat family member 2A
Synonyms: ENSMUSG00000044873, OTTMUSG00000020804, Heatr7b1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 100040766
Homologene: 85300
Abo
Name: ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase, transferase B, alpha 1-3-galactosyltransferase)
Synonyms: cis-AB transferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 80908
HGNC: HGNC:79
Homologene: 69306
Myh2
Name: myosin, heavy polypeptide 2, skeletal muscle, adult
Synonyms: MyHC-IIa, MHC2A, Myhs-f, Myhs-f1, Myhsf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17882
HGNC: HGNC:7572
Homologene: 23019
Ccdc180
Name: coiled-coil domain containing 180
Synonyms: LOC381522, E230008N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381522
Homologene: 117988
Entpd7
Name: ectonucleoside triphosphate diphosphohydrolase 7
Synonyms: LALP1, 2810003F23Rik, 1810020C02Rik, 1810012B13Rik, Lysal2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 93685
Homologene: 122202
Syde2
Name: synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
Synonyms: C430017H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214804
Homologene: 19035
Megf8
Name: multiple EGF-like-domains 8
Synonyms: Egfl4, b2b288Clo, b2b1702Clo, m687Ddg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269878
HGNC: HGNC:3233
Homologene: 15988
Cd209g
Name: CD209g antigen
Synonyms: 2310066I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70192
Homologene: 137382
Dhx30
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 30
Synonyms: Ddx30, 2810477H02Rik, C130058C04Rik, helG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72831
Homologene: 15779
Spag17
Name: sperm associated antigen 17
Synonyms: 4931427F14Rik, PF6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74362
Homologene: 52601
Trim28
Name: tripartite motif-containing 28
Synonyms: KRIP-1, KAP-1, Tif1b, MommeD9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21849
Homologene: 21175
Ppip5k1
Name: diphosphoinositol pentakisphosphate kinase 1
Synonyms: B430315C20Rik, Hisppd2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 327655
Homologene: 49395
Spg20
Name: spastic paraplegia 20, spartin (Troyer syndrome) homolog (human)
Synonyms: TAHCCP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229285
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: Neu, Neu oncogene, c-erbB2, HER-2, ErbB-2, HER2, c-neu, l11Jus8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Sox1
Name: SRY (sex determining region Y)-box 1
Synonyms: Sox-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20664
Homologene: 133765
Cyp2c55
Name: cytochrome P450, family 2, subfamily c, polypeptide 55
Synonyms: 2010318C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72082
HGNC: HGNC:2620
Homologene: 133567
Vmn1r84
Name: vomeronasal 1 receptor 84
Synonyms: V1rg11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171267
Homologene: 74328
Lax1
Name: lymphocyte transmembrane adaptor 1
Synonyms: E430019B13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240754
Homologene: 49504
Habp2
Name: hyaluronic acid binding protein 2
Synonyms: FSAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226243
HGNC: HGNC:4798
Homologene: 3050
Zfp488
Name: zinc finger protein 488
Synonyms: LOC382867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 382867
Homologene: 17688
Or5k17
Name: olfactory receptor family 5 subfamily K member 17
Synonyms: GA_x54KRFPKG5P-55145984-55145034, MOR184-4, Olfr181
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 259001
Homologene: 74112
Cyp2d34
Name: cytochrome P450, family 2, subfamily d, polypeptide 34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223706
VEGA: 15
HGNC: HGNC:2625
Homologene: 86099
Cdh22
Name: cadherin 22
Synonyms: PB-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 104010
Homologene: 23148
Or12d2
Name: olfactory receptor family 12 subfamily D member 2
Synonyms: MOR250-4, GA_x6K02T2PSCP-1775063-1774137, Olfr102
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258218
Homologene: 133728
Sertad3
Name: SERTA domain containing 3
Synonyms: Rbt1, Sei3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170742
Homologene: 8361
Tspan1
Name: tetraspanin 1
Synonyms: 9030418M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66805
Homologene: 21170
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 88,246,042 bp
  • T to C, chromosome 1 at 132,939,114 bp
  • C to T, chromosome 1 at 133,683,036 bp
  • T to C, chromosome 1 at 182,329,910 bp
  • T to A, chromosome 2 at 26,843,800 bp
  • G to T, chromosome 2 at 109,896,445 bp
  • C to G, chromosome 2 at 112,675,873 bp
  • A to G, chromosome 2 at 120,704,155 bp
  • C to T, chromosome 2 at 121,337,627 bp
  • T to C, chromosome 2 at 165,157,253 bp
  • A to T, chromosome 2 at 165,842,889 bp
  • G to T, chromosome 2 at 166,873,945 bp
  • A to G, chromosome 3 at 20,035,261 bp
  • G to T, chromosome 3 at 55,117,736 bp
  • T to C, chromosome 3 at 84,445,102 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to T, chromosome 3 at 100,049,230 bp
  • C to T, chromosome 3 at 133,082,068 bp
  • A to G, chromosome 3 at 146,015,725 bp
  • T to A, chromosome 4 at 45,904,560 bp
  • T to C, chromosome 4 at 106,379,224 bp
  • C to T, chromosome 4 at 116,167,035 bp
  • T to A, chromosome 4 at 126,368,593 bp
  • T to C, chromosome 4 at 136,542,193 bp
  • T to C, chromosome 5 at 22,227,630 bp
  • C to T, chromosome 5 at 76,549,570 bp
  • A to T, chromosome 5 at 139,546,689 bp
  • G to A, chromosome 6 at 72,436,244 bp
  • T to A, chromosome 6 at 115,547,987 bp
  • T to C, chromosome 7 at 12,361,930 bp
  • T to C, chromosome 7 at 13,028,558 bp
  • T to C, chromosome 7 at 25,337,775 bp
  • A to G, chromosome 7 at 27,476,695 bp
  • G to T, chromosome 7 at 92,861,478 bp
  • A to T, chromosome 8 at 4,135,955 bp
  • A to G, chromosome 8 at 12,396,719 bp
  • T to C, chromosome 9 at 110,084,397 bp
  • G to A, chromosome 11 at 33,479,189 bp
  • T to C, chromosome 11 at 50,196,932 bp
  • T to C, chromosome 11 at 60,865,328 bp
  • G to T, chromosome 11 at 67,179,404 bp
  • A to T, chromosome 11 at 86,720,348 bp
  • T to G, chromosome 11 at 98,435,297 bp
  • G to A, chromosome 12 at 4,624,927 bp
  • G to A, chromosome 13 at 102,739,321 bp
  • G to T, chromosome 13 at 102,853,869 bp
  • T to A, chromosome 14 at 33,971,153 bp
  • C to T, chromosome 14 at 55,775,178 bp
  • C to T, chromosome 14 at 56,460,001 bp
  • A to G, chromosome 15 at 82,618,608 bp
  • A to G, chromosome 16 at 22,869,446 bp
  • T to C, chromosome 16 at 58,926,120 bp
  • T to C, chromosome 17 at 27,786,262 bp
  • T to A, chromosome 17 at 37,314,274 bp
  • A to T, chromosome 18 at 60,963,928 bp
  • T to A, chromosome 18 at 80,969,840 bp
  • A to G, chromosome 19 at 17,000,020 bp
  • T to A, chromosome 19 at 39,035,434 bp
  • G to A, chromosome 19 at 43,691,158 bp
  • G to T, chromosome 19 at 56,311,855 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4022 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040956-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.