Strain Name:
C57BL/6J-MtgxR4029Btlr/Mmmh
Stock Number:
040959-MU
Citation ID:
RRID:MMRRC_040959-MU
Other Names:
R4029 (G1), C57BL/6J-MtgxR4029Btlr
Major Collection:

Gene Information

Lefty1
Name: left right determination factor 1
Synonyms: lefty-1, Stra3, Lefty, Ebaf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13590
HGNC: HGNC:6552
Homologene: 49231
Nup35
Name: nucleoporin 35
Synonyms: 5330402E05Rik, 2310006I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69482
Homologene: 44517
Dip2b
Name: disco interacting protein 2 homolog B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239667
Homologene: 72227
Rcn1
Name: reticulocalbin 1
Synonyms: Rcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19672
HGNC: HGNC:9934
Homologene: 20637
Gpr68
Name: G protein-coupled receptor 68
Synonyms: OGR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238377
HGNC: HGNC:4519
Homologene: 2603
Shisal2a
Name: shisa like 2A
Synonyms: OTTMUSG00000008243, Fam159a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 545667
Homologene: 82675
Psmd2
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
Synonyms: TEG-190, Tex190, 9430095H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 21762
HGNC: HGNC:9559
Homologene: 2101
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53614
Homologene: 9622
Ston2
Name: stonin 2
Synonyms: 4933401N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 108800
Homologene: 84338
Phlpp1
Name: PH domain and leucine rich repeat protein phosphatase 1
Synonyms: Plekhe1, Phlpp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98432
Homologene: 11015
Ank
Name: progressive ankylosis
Synonyms: D15Ertd221e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11732
Homologene: 10664
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Pkd1l3
Name: polycystic kidney disease 1 like 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244646
Homologene: 14627
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Oog4
Name: oogenesin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242737
Bfsp1
Name: beaded filament structural protein 1, in lens-CP94
Synonyms: filensin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12075
HGNC: HGNC:1040
Homologene: 922
Atp9a
Name: ATPase, class II, type 9A
Synonyms: Class II, IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11981
Homologene: 69194
Pld2
Name: phospholipase D2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18806
HGNC: HGNC:9068
Homologene: 55672
Dmrt2
Name: doublesex and mab-3 related transcription factor 2
Synonyms: Terra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226049
VEGA: 19
HGNC: HGNC:2935
Homologene: 17111
Nme4
Name: NME/NM23 nucleoside diphosphate kinase 4
Synonyms: NM23-M4, 2610027N22Rik, 2810024O08Rik, 5730493H09Rik, non-metastatic cells 4, protein expressed in
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56520
HGNC: HGNC:7852
Homologene: 3673
Krt17
Name: keratin 17
Synonyms: K17, Krt1-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16667
HGNC: HGNC:6427
Homologene: 363
Syt10
Name: synaptotagmin X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54526
Homologene: 10303
Pramel28
Name: PRAME like 28
Synonyms: Gm13101
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 626922
Niban1
Name: niban apoptosis regulator 1
Synonyms: Niban, Fam129a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 63913
Homologene: 62170
Ube4a
Name: ubiquitination factor E4A
Synonyms: UFD2b, 9930123J21Rik, 4732444G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 140630
Homologene: 3517
Cenpq
Name: centromere protein Q
Synonyms: 2610528M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 83815
Homologene: 49525
Nck2
Name: non-catalytic region of tyrosine kinase adaptor protein 2
Synonyms: NCKbeta, Grb4, 4833426I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17974
HGNC: HGNC:7665
Homologene: 20794
Ly6g6d
Name: lymphocyte antigen 6 complex, locus G6D
Synonyms: NG32, G6f, NG25, G6d, MEGT1, A930024F17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 114654
Homologene: 76442
Mrpl11
Name: mitochondrial ribosomal protein L11
Synonyms: 2410001P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66419
VEGA: 19
Homologene: 6768
Acsm4
Name: acyl-CoA synthetase medium-chain family member 4
Synonyms: O-MACS, OMACS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233801
Homologene: 72281
Dcun1d4
Name: DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100737
Homologene: 22867
Gabra4
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4
Synonyms: Gabra-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14397
HGNC: HGNC:4078
Homologene: 631
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 43,554,091 bp
  • T to A, chromosome 1 at 106,392,549 bp
  • G to A, chromosome 1 at 151,695,690 bp
  • T to C, chromosome 1 at 180,937,781 bp
  • A to G, chromosome 2 at 80,652,974 bp
  • G to T, chromosome 2 at 105,399,050 bp
  • G to A, chromosome 2 at 143,831,829 bp
  • A to T, chromosome 2 at 168,689,325 bp
  • T to C, chromosome 4 at 43,922,931 bp
  • G to T, chromosome 4 at 108,383,215 bp
  • A to T, chromosome 4 at 143,440,200 bp
  • T to A, chromosome 4 at 143,965,784 bp
  • G to T, chromosome 5 at 71,572,189 bp
  • A to G, chromosome 5 at 73,534,637 bp
  • A to G, chromosome 7 at 119,693,785 bp
  • G to A, chromosome 7 at 141,638,400 bp
  • T to A, chromosome 8 at 109,623,971 bp
  • G to A, chromosome 9 at 44,949,900 bp
  • T to C, chromosome 11 at 59,131,646 bp
  • A to G, chromosome 11 at 70,554,905 bp
  • T to A, chromosome 11 at 100,257,523 bp
  • T to C, chromosome 12 at 91,648,263 bp
  • A to G, chromosome 12 at 100,879,216 bp
  • A to G, chromosome 15 at 27,544,257 bp
  • T to C, chromosome 15 at 89,814,538 bp
  • A to G, chromosome 15 at 100,186,172 bp
  • G to A, chromosome 16 at 20,663,205 bp
  • T to C, chromosome 17 at 26,094,222 bp
  • T to A, chromosome 17 at 35,071,660 bp
  • T to C, chromosome 17 at 40,927,249 bp
  • T to C, chromosome 19 at 4,963,313 bp
  • T to G, chromosome 19 at 25,678,134 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4029 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040959-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.