Strain Name:
C57BL/6J-MtgxR4079Btlr/Mmmh
Stock Number:
040976-MU
Citation ID:
RRID:MMRRC_040976-MU
Other Names:
R4079 (G1), C57BL/6J-MtgxR4079Btlr
Major Collection:

Gene Information

Slc12a1
Name: solute carrier family 12, member 1
Synonyms: Nkcc2, mBSC1, D630042G03Rik, urehr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20495
Homologene: 286
Hgs
Name: HGF-regulated tyrosine kinase substrate
Synonyms: Hrs, tn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15239
HGNC: HGNC:4897
Homologene: 37954
Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Fzd9
Name: frizzled class receptor 9
Synonyms: mfz9, frizzled 9, Fz9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14371
HGNC: HGNC:4047
Homologene: 2619
Ptprk
Name: protein tyrosine phosphatase, receptor type, K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Tjp2
Name: tight junction protein 2
Synonyms: ZO-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21873
VEGA: 19
Homologene: 3541
Mfn1
Name: mitofusin 1
Synonyms: 6330416C07Rik, 2310002F04Rik, D3Ertd265e, HR2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67414
Homologene: 11481
Lrig3
Name: leucine-rich repeats and immunoglobulin-like domains 3
Synonyms: 9030421L11Rik, 9130004I02Rik, 9430095K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 320398
VEGA: 10
Homologene: 62452
Phax
Name: phosphorylated adaptor for RNA export
Synonyms: p55, 4933427L19Rik, 2810055C14Rik, D18Ertd65e, phosphorylation regulated, Phax, Rnuxa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 56698
Homologene: 10558
Arhgef12
Name: Rho guanine nucleotide exchange factor (GEF) 12
Synonyms: LARG, 2310014B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69632
VEGA: 9
Homologene: 9088
Fgf1
Name: fibroblast growth factor 1
Synonyms: fibroblast growth factor 1 (acidic), Fgf-1, Fgfa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 14164
HGNC: HGNC:3665
Homologene: 625
Armc9
Name: armadillo repeat containing 9
Synonyms: 5730415N24Rik, 3830422A13Rik, 4831423D23Rik, 4930438O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78795
Homologene: 11847
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237898
Homologene: 13066
Btaf1
Name: B-TFIID TATA-box binding protein associated factor 1
Synonyms: E430027O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107182
Homologene: 31978
Ung
Name: uracil DNA glycosylase
Synonyms: UNG1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22256
Homologene: 6585
Diaph1
Name: diaphanous related formin 1
Synonyms: p140mDia, Drf1, Dia1, D18Wsu154e, mDia1, Diap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13367
HGNC: HGNC:2876
Homologene: 129567
Hnrnpul1
Name: heterogeneous nuclear ribonucleoprotein U-like 1
Synonyms: E1B-AP5, E1BAP5, E130317O14Rik, Hnrnpul, Hnrpul1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232989
Homologene: 31419
Fcho2
Name: FCH domain only 2
Synonyms: 5832424M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218503
VEGA: 13
Homologene: 9030
Ago3
Name: argonaute RISC catalytic subunit 3
Synonyms: argonaute 3, eIF2C3, C130014L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214150
Homologene: 49799
Ankfy1
Name: ankyrin repeat and FYVE domain containing 1
Synonyms: Ankhzn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11736
Homologene: 9491
Ccdc18
Name: coiled-coil domain containing 18
Synonyms: 1700021E15Rik, 4932411G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73254
Homologene: 35455
Rasgrp1
Name: RAS guanyl releasing protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19419
HGNC: HGNC:9878
Homologene: 4195
Stim2
Name: stromal interaction molecule 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 116873
Homologene: 32490
Cdc45
Name: cell division cycle 45
Synonyms: Cdc45l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12544
HGNC: HGNC:1739
Homologene: 2616
Scyl2
Name: SCY1-like 2 (S. cerevisiae)
Synonyms: D10Ertd802e, CVAK104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 213326
Homologene: 13349
Jak1
Name: Janus kinase 1
Synonyms: C130039L05Rik, BAP004
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16451
HGNC: HGNC:6190
Homologene: 1678
Flii
Name: flightless I actin binding protein
Synonyms: 3632430F08Rik, Fliih
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14248
HGNC: HGNC:3750
Homologene: 11092
C3
Name: complement component 3
Synonyms: complement factor 3, acylation stimulating protein, Plp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Cadm3
Name: cell adhesion molecule 3
Synonyms: Tsll1, BIgR, Necl1, Necl-1, SynCAM3, Igsf4b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 94332
Homologene: 10919
Nfatc3
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
Synonyms: NFAT4, NFATx, D8Ertd281e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18021
HGNC: HGNC:7777
Homologene: 27827
Hbs1l
Name: Hbs1-like (S. cerevisiae)
Synonyms: eRFS, 2810035F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 56422
VEGA: 10
HGNC: HGNC:4834
Homologene: 68525
Patl1
Name: protein associated with topoisomerase II homolog 1 (yeast)
Synonyms: Pat1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225929
VEGA: 19
Homologene: 82269
Slc29a3
Name: solute carrier family 29 (nucleoside transporters), member 3
Synonyms: Ent3, 4933435C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71279
Homologene: 56805
St6galnac2
Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2
Synonyms: ST6GalNAc II, Siat7, Siat7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20446
Homologene: 4714
Enpp1
Name: ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms: PC-1, NPP1, Ly-41, Pca-1, Pca, Npps, Pdnp1, twy, CD203c, E-NPP1, 4833416E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18605
HGNC: HGNC:3356
Homologene: 38151
Cox16
Name: cytochrome c oxidase assembly protein 16
Synonyms: 1810055I05Rik, 1810020G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66272
Homologene: 9520
Prol1
Name: proline rich, lacrimal 1
Synonyms: Muc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17830
Apeh
Name: acylpeptide hydrolase
Synonyms: N-acylaminoacyl peptide hydrolase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235606
HGNC: HGNC:586
Homologene: 1240
Fcer1a
Name: Fc receptor, IgE, high affinity I, alpha polypeptide
Synonyms: Fce1a, Fcr-5, FcERI
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14125
HGNC: HGNC:3609
Homologene: 1516
Kpna7
Name: karyopherin alpha 7 (importin alpha 8)
Synonyms: LOC381686, importin alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381686
Homologene: 73467
Lrpap1
Name: low density lipoprotein receptor-related protein associated protein 1
Synonyms: RAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16976
HGNC: HGNC:6701
Homologene: 37612
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Ap4b1
Name: adaptor-related protein complex AP-4, beta 1
Synonyms: AP-4 beta-4, 1810038H16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67489
HGNC: HGNC:572
Homologene: 38203
Olfr1037
Name: olfactory receptor 1037
Synonyms: GA_x6K02T2Q125-47560740-47559775, MOR171-52, MOR256-34P
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259151
Homologene: 66572
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
Tns1
Name: tensin 1
Synonyms: 1200014E20Rik, E030018G17Rik, 1110018I21Rik, Tns, E030037J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21961
Homologene: 11219
Bnc1
Name: basonuclin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12173
HGNC: HGNC:1081
Homologene: 31048
Olfr686
Name: olfactory receptor 686
Synonyms: GA_x6K02T2PBJ9-7832633-7831680, MOR35-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259072
Homologene: 17487
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Snap47
Name: synaptosomal-associated protein, 47
Synonyms: SNAP-47, 1110031B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67826
Homologene: 14206
Mpeg1
Name: macrophage expressed gene 1
Synonyms: Mpg-1, MPS1, Perforin-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17476
VEGA: 19
Homologene: 11216
Ranbp3l
Name: RAN binding protein 3-like
Synonyms: C130037N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223332
VEGA: 15
Homologene: 35407
F13b
Name: coagulation factor XIII, beta subunit
Synonyms: Cf-13b, Cf13b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14060
HGNC: HGNC:3534
Homologene: 1512
Cfap57
Name: cilia and flagella associated protein 57
Synonyms: C130004B06Rik, LOC384050, 1110020C03Rik, Wdr65
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 68625
Homologene: 51350
Cbfa2t3
Name: CBFA2/RUNX1 translocation partner 3
Synonyms: MTGR2, ETO-2, A630044F12Rik, Eto2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12398
HGNC: HGNC:1537
Homologene: 74543
Mog
Name: myelin oligodendrocyte glycoprotein
Synonyms: B230317G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17441
HGNC: HGNC:7197
Homologene: 111009
Mx2
Name: MX dynamin-like GTPase 2
Synonyms: Mx-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17858
Pdss2
Name: prenyl (solanesyl) diphosphate synthase, subunit 2
Synonyms: 5430420P03Rik, kd, mDLP1, PLMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71365
Homologene: 56885
Ptpru
Name: protein tyrosine phosphatase, receptor type, U
Synonyms: Ptprl, RPTPlambda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19273
HGNC: HGNC:9683
Homologene: 4168
Cyp2a4
Name: cytochrome P450, family 2, subfamily a, polypeptide 4
Synonyms: Cyp15a1, D7Ucla4, testosterone 15alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13086
Homologene: 85917
Arl5c
Name: ADP-ribosylation factor-like 5C
Synonyms: Arl12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217151
Homologene: 28456
Syce1
Name: synaptonemal complex central element protein 1
Synonyms: 4933406J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74075
Homologene: 77044
Gm10354
Name: predicted gene 10354
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100503549
Homologene: 69402
Serpina3a
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3A
Synonyms: 4933406L18Rik, antitrypsin, alpha-1 antiproteinase,
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74069
HGNC: HGNC:16
Cnga3
Name: cyclic nucleotide gated channel alpha 3
Synonyms: CNG3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12790
HGNC: HGNC:2150
Homologene: 994
Ptprv
Name: protein tyrosine phosphatase, receptor type, V
Synonyms: mOST-PTP, Esp, OST-PTP, OST
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13924
Homologene: 7306
Rapgefl1
Name: Rap guanine nucleotide exchange factor (GEF)-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 268480
Homologene: 41126
Pnck
Name: pregnancy upregulated non-ubiquitously expressed CaM kinase
Synonyms: Punc, Bstk3, Camk1b, CaMKIbeta2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 93843
Homologene: 128728
Gm14471
Name: predicted gene 14471
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Trav6-3
Name: T cell receptor alpha variable 6-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328483
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 37,241,865 bp
  • G to A, chromosome 1 at 73,995,308 bp
  • A to T, chromosome 1 at 86,213,129 bp
  • A to G, chromosome 1 at 135,110,430 bp
  • T to A, chromosome 1 at 139,501,770 bp
  • C to T, chromosome 1 at 173,225,353 bp
  • A to G, chromosome 1 at 173,341,669 bp
  • G to A, chromosome 2 at 30,309,878 bp
  • A to G, chromosome 2 at 86,085,312 bp
  • A to G, chromosome 2 at 117,285,029 bp
  • A to T, chromosome 2 at 125,200,623 bp
  • T to G, chromosome 3 at 32,542,849 bp
  • T to A, chromosome 3 at 103,813,378 bp
  • G to T, chromosome 4 at 101,184,364 bp
  • C to A, chromosome 4 at 118,598,997 bp
  • C to G, chromosome 4 at 126,353,680 bp
  • A to T, chromosome 4 at 131,798,710 bp
  • A to T, chromosome 5 at 14,977,649 bp
  • A to G, chromosome 5 at 35,096,037 bp
  • A to T, chromosome 5 at 54,104,475 bp
  • T to C, chromosome 5 at 72,503,883 bp
  • A to G, chromosome 5 at 88,328,216 bp
  • C to T, chromosome 5 at 108,158,528 bp
  • G to T, chromosome 5 at 114,130,623 bp
  • A to G, chromosome 5 at 135,249,636 bp
  • A to G, chromosome 5 at 145,005,927 bp
  • C to T, chromosome 7 at 25,726,875 bp
  • A to G, chromosome 7 at 26,307,366 bp
  • T to C, chromosome 7 at 81,973,760 bp
  • A to T, chromosome 7 at 105,204,021 bp
  • C to A, chromosome 7 at 140,779,896 bp
  • T to C, chromosome 8 at 106,079,491 bp
  • A to G, chromosome 8 at 122,647,695 bp
  • T to C, chromosome 9 at 42,975,292 bp
  • G to A, chromosome 9 at 108,089,552 bp
  • T to C, chromosome 10 at 21,352,602 bp
  • A to G, chromosome 10 at 24,669,007 bp
  • G to A, chromosome 10 at 28,263,512 bp
  • A to T, chromosome 10 at 43,402,522 bp
  • G to A, chromosome 10 at 60,716,316 bp
  • A to T, chromosome 10 at 89,640,596 bp
  • A to C, chromosome 10 at 126,009,787 bp
  • C to T, chromosome 11 at 4,491,057 bp
  • A to G, chromosome 11 at 59,038,363 bp
  • C to T, chromosome 11 at 59,428,551 bp
  • C to A, chromosome 11 at 60,710,284 bp
  • C to A, chromosome 11 at 72,690,009 bp
  • T to A, chromosome 11 at 85,039,229 bp
  • A to T, chromosome 11 at 97,993,501 bp
  • A to G, chromosome 11 at 98,849,977 bp
  • A to T, chromosome 11 at 116,681,898 bp
  • T to A, chromosome 11 at 120,483,048 bp
  • A to T, chromosome 12 at 81,474,335 bp
  • C to T, chromosome 12 at 104,119,675 bp
  • A to G, chromosome 13 at 98,755,612 bp
  • C to T, chromosome 14 at 12,457,702 bp
  • T to C, chromosome 14 at 24,148,260 bp
  • T to C, chromosome 14 at 53,430,080 bp
  • A to G, chromosome 15 at 9,060,757 bp
  • A to T, chromosome 16 at 18,811,360 bp
  • A to G, chromosome 16 at 97,556,036 bp
  • A to G, chromosome 17 at 37,012,410 bp
  • C to T, chromosome 17 at 57,205,303 bp
  • T to A, chromosome 18 at 37,853,583 bp
  • A to G, chromosome 18 at 38,892,764 bp
  • A to G, chromosome 18 at 56,575,979 bp
  • C to T, chromosome 19 at 11,931,630 bp
  • A to G, chromosome 19 at 12,462,270 bp
  • A to T, chromosome 19 at 24,108,818 bp
  • A to G, chromosome 19 at 36,986,479 bp
  • A to T, chromosome X at 73,658,155 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4079 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040976-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.