Strain Name:
C57BL/6J-MtgxR4169Btlr/Mmmh
Stock Number:
041010-MU
Citation ID:
RRID:MMRRC_041010-MU
Other Names:
R4169 (G1), C57BL/6J-MtgxR4169Btlr
Major Collection:

Gene Information

Acot7
Name: acyl-CoA thioesterase 7
Synonyms: 2410041A17Rik, Bach, AU014716
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70025
Homologene: 15780
Ddx50
Name: DExD box helicase 50
Synonyms: GU2, RH-II/Gubeta, 4933429B04Rik, 8430408E17Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 94213
VEGA: 10
Homologene: 56986
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217127
Homologene: 5134
Hectd1
Name: HECT domain E3 ubiquitin protein ligase 1
Synonyms: A630086P08Rik, opm, b2b327Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 207304
VEGA: 12
Homologene: 9115
Snapc1
Name: small nuclear RNA activating complex, polypeptide 1
Synonyms: 2700033G17Rik, 9630050P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 75627
VEGA: 12
Homologene: 2317
Rfng
Name: RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: radical fringe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 19719
HGNC: HGNC:9974
Homologene: 56433
Cmip
Name: c-Maf inducing protein
Synonyms: 5830471E12Rik, 4933407C03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 74440
Homologene: 18869
Nop53
Name: NOP53 ribosome biogenesis factor
Synonyms: 9430097C02Rik, Gltscr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 68077
HGNC: HGNC:4333
Homologene: 44130
Gak
Name: cyclin G associated kinase
Synonyms: D130045N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231580
HGNC: HGNC:4113
Homologene: 3846
Tmem178
Name: transmembrane protein 178
Synonyms: 2810417M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68027
VEGA: 17
Homologene: 12226
Gpr155
Name: G protein-coupled receptor 155
Synonyms: DEPDC3, PGR22, 1110017O10Rik, F730029F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 68526
Homologene: 16584
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Hmcn1
Name: hemicentin 1
Synonyms: LOC240793, EG545370
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545370
Homologene: 23741
Piezo2
Name: piezo-type mechanosensitive ion channel component 2
Synonyms: 9430028L06Rik, 9030411M15Rik, Fam38b2, Piezo2, Fam38b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 667742
Homologene: 49695
Slc9a5
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 5
Synonyms: LOC277973
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 277973
Homologene: 31247
Pkdrej
Name: polycystin (PKD) family receptor for egg jelly
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18766
VEGA: 15
HGNC: HGNC:9015
Homologene: 4427
Man2c1
Name: mannosidase, alpha, class 2C, member 1
Synonyms: 1110025H24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 73744
HGNC: HGNC:6827
Homologene: 4887
Hrc
Name: histidine rich calcium binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 15464
HGNC: HGNC:5178
Homologene: 137234
Btn1a1
Name: butyrophilin, subfamily 1, member A1
Synonyms: Btn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 12231
HGNC: HGNC:1135
Homologene: 1312
Krt81
Name: keratin 81
Synonyms: Krt2-19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 64818
VEGA: 15
HGNC: HGNC:6458
Homologene: 55645
Col4a2
Name: collagen, type IV, alpha 2
Synonyms: Col4a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12827
HGNC: HGNC:2203
Homologene: 1390
Ampd1
Name: adenosine monophosphate deaminase 1
Synonyms: Ampd-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229665
HGNC: HGNC:468
Homologene: 20
Tbc1d31
Name: TBC1 domain family, member 31
Synonyms: LOC210544, D330013L20Rik, Wdr67
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 210544
Homologene: 17089
Elk3
Name: ELK3, member of ETS oncogene family
Synonyms: Erp, Sap-2, Net, D430049E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13713
HGNC: HGNC:3325
Homologene: 3833
Klk14
Name: kallikrein related-peptidase 14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 317653
HGNC: HGNC:6362
Homologene: 69348
Rabl2
Name: RAB, member RAS oncogene family-like 2
Synonyms: 1110031N17Rik, Rabl2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68708
Homologene: 90913
Rexo5
Name: RNA exonuclease 5
Synonyms: 2610020H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434234
Homologene: 12803
Olfr482
Name: olfactory receptor 482
Synonyms: GA_x6K02T2PBJ9-10424354-10423383, MOR204-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258728
Homologene: 133604
Birc3
Name: baculoviral IAP repeat-containing 3
Synonyms: MIAP2, IAP2, Api2, cIAP2, cIAP-2, HIAP2, MIHC, RNF49
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11796
HGNC: HGNC:591
Homologene: 899
Rps6ka4
Name: ribosomal protein S6 kinase, polypeptide 4
Synonyms: MSK2, 1110069D02Rik, 90kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56613
VEGA: 19
Homologene: 69288
Pcdh1
Name: protocadherin 1
Synonyms: 2010005A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75599
HGNC: HGNC:8655
Homologene: 12613
Gnat3
Name: guanine nucleotide binding protein, alpha transducing 3
Synonyms: Gtn, Ggust, alpha-gustducin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242851
Homologene: 24284
Vps25
Name: vacuolar protein sorting 25
Synonyms: 1110020N13Rik, D11Wsu68e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 28084
Homologene: 6303
Olfr281
Name: olfactory receptor 281
Synonyms: GA_x6K02T2NBG7-5275017-5274082, MOR160-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 258277
Homologene: 133703
Sox1ot
Name: Sox1 overlapping transcript
Synonyms: Gm5607
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 434280
Zkscan14
Name: zinc finger with KRAB and SCAN domains 14
Synonyms: 2810437E14Rik, 2310046C23Rik, Zfp99
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 67235
Homologene: 11342
Olfr220
Name: olfactory receptor 220
Synonyms: GA_x6K02T2P20D-20731742-20730694, GA_x6K02SYWY4V-595-239, MOR103-13P, MOR103-17, EG546747, Olfr413-ps1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 546747
Homologene: 67062
Samd11
Name: sterile alpha motif domain containing 11
Synonyms: mr-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 231004
VEGA: 4
Homologene: 34983
Trav16n
Name: T cell receptor alpha variable 16n
Synonyms: Gm16837
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100126459
RP23-83N21.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Mthfr-ps1
Name: 5,10-methylenetetrahydrofolate reductase, pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 387594
Ube2nl
Name: ubiquitin-conjugating enzyme E2N-like
Synonyms: EG620934
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620934
Ighv1-53
Name: immunoglobulin heavy variable 1-53
Synonyms: V23-D-J-C mu, AB069917
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 780931
Gm5445
Name: predicted gene 5445
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432725
VEGA: 13
Homologene: 138847
Krtap6-1
Name: keratin associated protein 6-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16700
VEGA: 16
Pcdhga12
Name: protocadherin gamma subfamily A, 12
Synonyms: pc2c, Pcdh13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93724
HGNC: HGNC:8699
Homologene: 134588
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 150,595,999 bp
  • T to A, chromosome 1 at 174,449,596 bp
  • C to A, chromosome 2 at 73,351,829 bp
  • T to A, chromosome 2 at 76,872,759 bp
  • T to C, chromosome 2 at 76,932,565 bp
  • T to C, chromosome 2 at 125,363,952 bp
  • A to G, chromosome 3 at 62,339,814 bp
  • T to A, chromosome 3 at 103,094,841 bp
  • T to C, chromosome 4 at 152,217,793 bp
  • T to A, chromosome 4 at 156,247,746 bp
  • T to C, chromosome 5 at 18,003,864 bp
  • T to C, chromosome 5 at 78,474,589 bp
  • T to A, chromosome 5 at 108,584,245 bp
  • G to A, chromosome 5 at 145,196,175 bp
  • A to T, chromosome 7 at 15,942,319 bp
  • G to A, chromosome 7 at 43,692,077 bp
  • A to G, chromosome 7 at 45,336,757 bp
  • T to A, chromosome 7 at 61,549,632 bp
  • A to T, chromosome 7 at 108,095,384 bp
  • A to G, chromosome 7 at 119,827,398 bp
  • C to T, chromosome 8 at 11,429,391 bp
  • A to G, chromosome 8 at 12,430,544 bp
  • A to G, chromosome 8 at 105,357,400 bp
  • A to T, chromosome 8 at 117,456,917 bp
  • T to A, chromosome 9 at 7,849,683 bp
  • A to G, chromosome 9 at 57,138,026 bp
  • A to T, chromosome 10 at 62,640,770 bp
  • A to G, chromosome 10 at 93,265,335 bp
  • A to G, chromosome 11 at 95,280,472 bp
  • T to C, chromosome 11 at 101,254,092 bp
  • C to G, chromosome 11 at 120,783,946 bp
  • T to C, chromosome 12 at 51,790,225 bp
  • T to A, chromosome 12 at 73,982,491 bp
  • T to A, chromosome 12 at 115,158,546 bp
  • A to G, chromosome 13 at 12,378,646 bp
  • A to G, chromosome 13 at 23,465,155 bp
  • A to T, chromosome 14 at 53,346,418 bp
  • T to C, chromosome 15 at 57,955,351 bp
  • A to G, chromosome 15 at 85,816,314 bp
  • T to C, chromosome 15 at 89,590,379 bp
  • A to G, chromosome 15 at 98,456,997 bp
  • T to A, chromosome 15 at 101,461,312 bp
  • A to G, chromosome 16 at 89,031,696 bp
  • C to T, chromosome 17 at 80,944,803 bp
  • A to G, chromosome 18 at 37,766,414 bp
  • A to T, chromosome 18 at 38,198,305 bp
  • T to C, chromosome 18 at 63,050,604 bp
  • A to G, chromosome 19 at 6,831,820 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4169 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041010-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.