Strain Name:
C57BL/6J-MtgxR4196Btlr/Mmmh
Stock Number:
041027-MU
Citation ID:
RRID:MMRRC_041027-MU
Other Names:
R4196 (G1), C57BL/6J-MtgxR4196Btlr
Major Collection:

Gene Information

Gulo
Name: gulonolactone (L-) oxidase
Synonyms: L-gulono-gamma-lactone oxidase, sfx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 268756
VEGA: 14
HGNC: HGNC:4695
Homologene: 6566
Ntf3
Name: neurotrophin 3
Synonyms: NT3, Ntf-3, NT-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 18205
HGNC: HGNC:8023
Homologene: 1896
Shprh
Name: SNF2 histone linker PHD RING helicase
Synonyms: D230017O13Rik, 2610103K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 268281
Homologene: 6489
Sept4
Name: septin 4
Synonyms: Bh5, cell division control-related protein 2b, ARTS, Pnutl2, septin H5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: b2b1562Clo, my, 6030440P17Rik, ne, 8430406N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242022
Homologene: 18454
Prepl
Name: prolyl endopeptidase-like
Synonyms: 9530014L06Rik, D030028O16Rik, 2810457N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 213760
VEGA: 17
Homologene: 15481
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: 2610507P14Rik, KAT9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 74195
VEGA: 14
Homologene: 7105
Usp5
Name: ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms: Ucht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22225
Homologene: 55758
Cnot2
Name: CCR4-NOT transcription complex, subunit 2
Synonyms: 2600016M12Rik, 2810470K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 72068
HGNC: HGNC:7878
Homologene: 40953
Ptprg
Name: protein tyrosine phosphatase, receptor type, G
Synonyms: RPTPgamma, 5430405N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 19270
HGNC: HGNC:9671
Homologene: 2129
Eif4enif1
Name: eukaryotic translation initiation factor 4E nuclear import factor 1
Synonyms: A930019J01Rik, D11Ertd166e, Clast4, 2610509L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74203
Homologene: 10522
Ankle2
Name: ankyrin repeat and LEM domain containing 2
Synonyms: D5Ertd585e, 1110001J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 71782
Homologene: 35424
Elp1
Name: elongator complex protein 1
Synonyms: C78473, 3110040G09Rik, IKAP, Ikbkap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230233
HGNC: HGNC:5959
Homologene: 2699
Hspd1
Name: heat shock protein 1 (chaperonin)
Synonyms: Hsp60
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 15510
HGNC: HGNC:5261
Homologene: 1626
Baz1a
Name: bromodomain adjacent to zinc finger domain 1A
Synonyms: Gtl5, Wcrf180, Acf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 217578
HGNC: HGNC:960
Homologene: 45654
Bcl9
Name: B cell CLL/lymphoma 9
Synonyms: A330041G23Rik, 2610202E01Rik, 8030475K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 77578
HGNC: HGNC:1008
Homologene: 3191
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 171395
Homologene: 51376
Lrp3
Name: low density lipoprotein receptor-related protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 435965
HGNC: HGNC:6695
Homologene: 1745
Nlgn1
Name: neuroligin 1
Synonyms: Nlg1, NL1, 6330415N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 192167
Homologene: 56690
2610507B11Rik
Name: RIKEN cDNA 2610507B11 gene
Synonyms: E1, D11Bhm178e, D11Bhm179e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 72503
Homologene: 34730
Slc3a1
Name: solute carrier family 3, member 1
Synonyms: D2H, NTAA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20532
VEGA: 17
Homologene: 37289
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Wnk4
Name: WNK lysine deficient protein kinase 4
Synonyms: Prkwnk4, 2010002J11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69847
Homologene: 13020
Pde8a
Name: phosphodiesterase 8A
Synonyms: Pde8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18584
HGNC: HGNC:8793
Homologene: 1957
Rab11fip2
Name: RAB11 family interacting protein 2 (class I)
Synonyms: Rab11-FIP2, 4930470G04Rik, A830046J09Rik, nRip11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 74998
Homologene: 8937
Fbll1
Name: fibrillarin-like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237730
Homologene: 28185
Trim75
Name: tripartite motif-containing 75
Synonyms: LOC333307
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 333307
Homologene: 53452
Lipg
Name: lipase, endothelial
Synonyms: 3110013K01Rik, endothelial lipase, EL, mEDL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16891
VEGA: 18
HGNC: HGNC:6623
Homologene: 21218
Itgav
Name: integrin alpha V
Synonyms: D430040G12Rik, 1110004F14Rik, 2610028E01Rik, vitronectin receptor alpha polypeptide (VNRA), alphav-integrin, CD51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 16410
HGNC: HGNC:6150
Homologene: 20510
Rasgef1c
Name: RasGEF domain family, member 1C
Synonyms: 9130006A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74563
Homologene: 17918
Tcp11l1
Name: t-complex 11 like 1
Synonyms: C130096D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 320554
Homologene: 41260
Acmsd
Name: amino carboxymuconate semialdehyde decarboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 266645
Homologene: 44520
Vmn1r225
Name: vomeronasal 1 receptor 225
Synonyms: V1re5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 171228
Mrps9
Name: mitochondrial ribosomal protein S9
Synonyms: 2310002A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 69527
Homologene: 32565
Aup1
Name: ancient ubiquitous protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 11993
HGNC: HGNC:891
Homologene: 7239
Zbtb9
Name: zinc finger and BTB domain containing 9
Synonyms: 3930402F13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 474156
Homologene: 45145
Rhbdd3
Name: rhomboid domain containing 3
Synonyms: 5730411O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 279766
HGNC: HGNC:1308
Homologene: 8175
Gm2396
Name: predicted gene 2396
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 105245043
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 24,534,052 bp
  • T to G, chromosome 1 at 42,901,094 bp
  • T to C, chromosome 1 at 55,086,909 bp
  • C to T, chromosome 1 at 68,343,855 bp
  • T to G, chromosome 1 at 127,749,194 bp
  • A to G, chromosome 2 at 83,768,327 bp
  • A to G, chromosome 2 at 104,699,964 bp
  • A to G, chromosome 3 at 25,434,392 bp
  • A to G, chromosome 3 at 53,539,268 bp
  • A to T, chromosome 3 at 97,216,368 bp
  • A to T, chromosome 4 at 56,755,353 bp
  • A to G, chromosome 5 at 110,244,543 bp
  • T to C, chromosome 6 at 83,055,230 bp
  • C to T, chromosome 6 at 124,824,938 bp
  • T to C, chromosome 6 at 126,102,175 bp
  • T to A, chromosome 7 at 35,203,410 bp
  • A to G, chromosome 7 at 81,306,697 bp
  • A to G, chromosome 8 at 64,982,764 bp
  • A to G, chromosome 9 at 88,917,662 bp
  • A to G, chromosome 10 at 11,207,860 bp
  • A to T, chromosome 10 at 116,501,304 bp
  • T to C, chromosome 11 at 3,243,186 bp
  • G to T, chromosome 11 at 5,099,460 bp
  • T to A, chromosome 11 at 8,909,929 bp
  • T to A, chromosome 11 at 35,797,872 bp
  • C to T, chromosome 11 at 49,971,455 bp
  • A to G, chromosome 11 at 78,263,556 bp
  • C to A, chromosome 11 at 87,588,772 bp
  • T to A, chromosome 11 at 101,269,631 bp
  • T to C, chromosome 12 at 54,911,415 bp
  • A to T, chromosome 14 at 12,122,002 bp
  • A to G, chromosome 14 at 65,548,002 bp
  • G to T, chromosome 14 at 65,988,253 bp
  • T to A, chromosome 17 at 20,502,975 bp
  • C to T, chromosome 17 at 26,973,879 bp
  • T to A, chromosome 17 at 85,060,878 bp
  • T to C, chromosome 17 at 85,081,154 bp
  • C to T, chromosome 18 at 74,945,831 bp
  • T to C, chromosome 19 at 59,935,781 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4196 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041027-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.