Strain Name:
C57BL/6J-MtgxR4201Btlr/Mmmh
Stock Number:
041031-MU
Citation ID:
RRID:MMRRC_041031-MU
Other Names:
R4201 (G1), C57BL/6J-MtgxR4201Btlr
Major Collection:

Gene Information

Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Nudt3
Name: nudix (nucleotide diphosphate linked moiety X)-type motif 3
Synonyms: diphosphoinositol polyphosphate phosphohydrolase, Dipp, 1110011B09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 56409
HGNC: HGNC:8050
Homologene: 31400
Gch1
Name: GTP cyclohydrolase 1
Synonyms: GTPCH, GTP-CH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14528
VEGA: 14
HGNC: HGNC:4193
Homologene: 132
Cd9
Name: CD9 antigen
Synonyms: Tspan29
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12527
HGNC: HGNC:1709
Homologene: 20420
Bag3
Name: BCL2-associated athanogene 3
Synonyms: Bis, Bcl-2-interacting death suppressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 29810
HGNC: HGNC:939
Homologene: 3162
Sec23a
Name: SEC23 homolog A, COPII coat complex component
Synonyms: Sec23r, Msec23
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20334
Homologene: 4642
Shroom3
Name: shroom family member 3
Synonyms: D5Ertd287e, Shrm, Shrm3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27428
Homologene: 9263
Dnmt3b
Name: DNA methyltransferase 3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13436
HGNC: HGNC:2979
Homologene: 56000
Als2
Name: alsin Rho guanine nucleotide exchange factor
Synonyms: 3222402C23Rik, Als2cr6, 9430073A21Rik, Alsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74018
HGNC: HGNC:443
Homologene: 23264
Parp4
Name: poly (ADP-ribose) polymerase family, member 4
Synonyms: VPARP, VAULT3, p193, PH5P, E230037B21Rik, Adprtl1, C030027K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 328417
HGNC: HGNC:271
Homologene: 124423
Syne1
Name: spectrin repeat containing, nuclear envelope 1
Synonyms: nesprin-1, SYNE-1, enaptin165, A330049M09Rik, C130039F11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 64009
VEGA: 10
Homologene: 52329
Ptprj
Name: protein tyrosine phosphatase, receptor type, J
Synonyms: Byp, DEP-1, Scc-1, Scc1, CD148, RPTPJ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19271
HGNC: HGNC:9673
Homologene: 2130
Cul7
Name: cullin 7
Synonyms: p193, 2510004L20Rik, p185, C230011P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66515
Homologene: 56683
Nfix
Name: nuclear factor I/X
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18032
HGNC: HGNC:7788
Homologene: 1872
Boc
Name: biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 117606
Homologene: 32819
Pgs1
Name: phosphatidylglycerophosphate synthase 1
Synonyms: 2610019F11Rik, 4933424M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 74451
Homologene: 5467
Camk1d
Name: calcium/calmodulin-dependent protein kinase ID
Synonyms: CKLiK, E030025C11Rik, A630059D12Rik, CaMKIdelta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227541
Homologene: 121858
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 319675
Homologene: 27936
Nbas
Name: neuroblastoma amplified sequence
Synonyms: 4933425L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 71169
VEGA: 12
Homologene: 41073
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, E030034C22Rik, B230373P09Rik, Dnahc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
Tbccd1
Name: TBCC domain containing 1
Synonyms: 5730478M09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70573
VEGA: 16
Homologene: 32392
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b1203Clo, b2b1279Clo, b2b598Clo, b2b1289Clo, b2b1727Clo, Dnahc11, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Cd101
Name: CD101 antigen
Synonyms: LOC381460, Igsf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 630146
HGNC: HGNC:5949
Homologene: 3142
Gpr22
Name: G protein-coupled receptor 22
Synonyms: 2900068K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73010
HGNC: HGNC:4477
Homologene: 18420
Atp1b2
Name: ATPase, Na+/K+ transporting, beta 2 polypeptide
Synonyms: Atpb-2, Amog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11932
HGNC: HGNC:805
Homologene: 36075
Plin4
Name: perilipin 4
Synonyms: S3-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57435
Homologene: 69311
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240776
Homologene: 16121
Vmn2r87
Name: vomeronasal 2, receptor 87
Synonyms: EG625131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 625131
Homologene: 129606
4921524L21Rik
Name: RIKEN cDNA 4921524L21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70901
VEGA: 18
Homologene: 78009
Olfr807
Name: olfactory receptor 807
Synonyms: GA_x6K02T2PULF-11434134-11433199, MOR117-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258931
Homologene: 27300
Ccdc172
Name: coiled-coil domain containing 172
Synonyms: 1700011F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75645
VEGA: 19
Homologene: 36871
Stx17
Name: syntaxin 17
Synonyms: 6330411F21Rik, 4833418L03Rik, 9030425C21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67727
Homologene: 9917
Olfr776
Name: olfactory receptor 776
Synonyms: GA_x6K02T2PULF-10947193-10948131, MOR111-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 404321
Homologene: 115559
Tmed7
Name: transmembrane p24 trafficking protein 7
Synonyms: 5830493P14Rik, 3930401E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 66676
Homologene: 45644
Tex11
Name: testis expressed gene 11
Synonyms: 4930565P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 83558
Homologene: 49962
Igkv2-95-2
Name: immunoglobulin kappa variable 2-95-2
Synonyms: Gm17533
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 692164
D10Bwg1379e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 59,180,154 bp
  • G to A, chromosome 1 at 140,425,332 bp
  • T to C, chromosome 2 at 5,354,776 bp
  • A to C, chromosome 2 at 90,463,095 bp
  • G to T, chromosome 2 at 153,670,417 bp
  • A to C, chromosome 3 at 101,018,685 bp
  • T to A, chromosome 4 at 48,158,870 bp
  • G to T, chromosome 5 at 92,943,086 bp
  • T to C, chromosome 6 at 68,648,118 bp
  • T to C, chromosome 6 at 125,462,394 bp
  • C to A, chromosome 7 at 128,546,157 bp
  • CAAAAA to CAAAA, chromosome 8 at 84,716,247 bp
  • T to C, chromosome 9 at 15,332,538 bp
  • A to G, chromosome 9 at 54,998,075 bp
  • A to T, chromosome 10 at 5,347,870 bp
  • A to G, chromosome 10 at 18,619,782 bp
  • T to C, chromosome 10 at 129,261,777 bp
  • A to T, chromosome 10 at 129,754,628 bp
  • T to C, chromosome 10 at 130,472,579 bp
  • A to G, chromosome 11 at 69,603,469 bp
  • A to G, chromosome 11 at 118,002,536 bp
  • G to T, chromosome 12 at 13,374,826 bp
  • A to T, chromosome 12 at 31,708,913 bp
  • T to C, chromosome 12 at 59,002,005 bp
  • T to C, chromosome 12 at 117,966,659 bp
  • A to G, chromosome 14 at 31,262,270 bp
  • T to C, chromosome 14 at 47,155,803 bp
  • C to A, chromosome 14 at 56,592,391 bp
  • A to G, chromosome 16 at 22,825,948 bp
  • T to C, chromosome 16 at 44,490,618 bp
  • T to C, chromosome 17 at 27,596,784 bp
  • C to T, chromosome 17 at 46,661,312 bp
  • T to G, chromosome 17 at 56,104,338 bp
  • A to G, chromosome 18 at 6,623,952 bp
  • A to G, chromosome 18 at 46,593,247 bp
  • G to A, chromosome 19 at 58,536,585 bp
  • C to A, chromosome X at 100,933,415 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4201 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041031-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.