Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR4207Btlr/Mmmh
Stock Number:
041036-MU
Citation ID:
RRID:MMRRC_041036-MU
Other Names:
R4207 (G1), C57BL/6J-MtgxR4207Btlr
Major Collection:

Strain Information

Sstr2
Name: somatostatin receptor 2
Synonyms: SSTR-2, sst2, Smstr2, Smstr-2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20606
Homologene: 37427
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Aff1
Name: AF4/FMR2 family, member 1
Synonyms: Af4, Rob, 9630032B01Rik, Mllt2h
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17355
HGNC: HGNC:7135
Homologene: 4340
Rbm5
Name: RNA binding motif protein 5
Synonyms: D030069N10Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 83486
HGNC: HGNC:9902
Homologene: 21177
Brk1
Name: BRICK1, SCAR/WAVE actin-nucleating complex subunit
Synonyms: 6720456B07Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 101314
Homologene: 10210
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 78286
Homologene: 52330
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to C, chromosome 1 at 88,277,215 bp
  • T to C, chromosome 1 at 97,469,895 bp
  • T to A, chromosome 1 at 171,054,075 bp
  • A to G, chromosome 1 at 174,581,955 bp
  • T to G, chromosome 1 at 175,993,875 bp
  • G to T, chromosome 2 at 68,220,920 bp
  • T to C, chromosome 2 at 90,909,755 bp
  • A to G, chromosome 3 at 93,279,862 bp
  • T to C, chromosome 4 at 34,806,079 bp
  • T to C, chromosome 4 at 35,709,810 bp
  • A to G, chromosome 4 at 87,227,205 bp
  • T to C, chromosome 5 at 103,818,988 bp
  • T to C, chromosome 5 at 106,618,276 bp
  • A to G, chromosome 6 at 48,478,293 bp
  • G to C, chromosome 6 at 83,122,333 bp
  • T to C, chromosome 6 at 113,615,844 bp
  • AGGG to AGG, chromosome 6 at 115,012,359 bp
  • G to T, chromosome 6 at 145,191,565 bp
  • T to A, chromosome 7 at 13,801,547 bp
  • T to A, chromosome 7 at 49,572,298 bp
  • T to A, chromosome 7 at 49,597,231 bp
  • T to C, chromosome 7 at 82,750,816 bp
  • T to A, chromosome 7 at 108,924,341 bp
  • A to G, chromosome 8 at 121,105,626 bp
  • A to G, chromosome 9 at 5,328,451 bp
  • A to G, chromosome 9 at 39,613,957 bp
  • A to G, chromosome 9 at 85,690,708 bp
  • A to G, chromosome 9 at 107,750,483 bp
  • G to T, chromosome 10 at 88,911,413 bp
  • T to C, chromosome 10 at 119,211,845 bp
  • A to C, chromosome 10 at 130,418,705 bp
  • A to G, chromosome 11 at 5,031,637 bp
  • A to G, chromosome 11 at 21,147,115 bp
  • A to T, chromosome 11 at 72,538,361 bp
  • G to A, chromosome 11 at 109,981,725 bp
  • A to C, chromosome 11 at 113,624,656 bp
  • G to T, chromosome 12 at 114,817,344 bp
  • A to T, chromosome 13 at 108,282,506 bp
  • G to T, chromosome 13 at 112,927,949 bp
  • G to T, chromosome 14 at 43,101,634 bp
  • A to G, chromosome 14 at 51,105,005 bp
  • A to G, chromosome 14 at 53,443,746 bp
  • T to G, chromosome 14 at 99,095,316 bp
  • G to T, chromosome 15 at 30,972,827 bp
  • C to T, chromosome 15 at 76,302,710 bp
  • T to C, chromosome 16 at 3,839,570 bp
  • C to T, chromosome 16 at 22,805,551 bp
  • T to A, chromosome 16 at 31,119,427 bp
  • C to T, chromosome 16 at 90,260,215 bp
  • G to A, chromosome 17 at 18,184,261 bp
  • G to A, chromosome 17 at 30,959,367 bp
  • C to T, chromosome 17 at 33,334,916 bp
  • A to T, chromosome 17 at 40,831,653 bp
  • T to C, chromosome 18 at 73,791,085 bp
  • T to C, chromosome 19 at 13,454,471 bp
  • T to C, chromosome X at 17,230,550 bp
  • G to A, chromosome X at 56,918,749 bp
  • A to T, chromosome X at 150,622,188 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4207 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041036-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.