Strain Name:
C57BL/6J-MtgxR4223Btlr/Mmmh
Stock Number:
041043-MU
Citation ID:
RRID:MMRRC_041043-MU
Other Names:
R4223 (G1), C57BL/6J-MtgxR4223Btlr
Major Collection:

Gene Information

Kifc3
Name: kinesin family member C3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 16582
HGNC: HGNC:6326
Homologene: 124421
Cmtm5
Name: CKLF-like MARVEL transmembrane domain containing 5
Synonyms: 2900052H21Rik, Cklfsf5, 1500005P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 67272
VEGA: 14
Homologene: 16322
Inpp5a
Name: inositol polyphosphate-5-phosphatase A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 212111
HGNC: HGNC:6076
Homologene: 4045
Ppcdc
Name: phosphopantothenoylcysteine decarboxylase
Synonyms: 8430432M10Rik, 1810057I13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 66812
VEGA: 9
Homologene: 11049
Rtn4
Name: reticulon 4
Synonyms: Nogo-B, Nogo-A, C130026I10Rik, NOGO, 1110020G17Rik, NgA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 68585
Homologene: 10743
Slc12a2
Name: solute carrier family 12, member 2
Synonyms: sodium/potassium/chloride cotransporters, sy-ns, Nkcc1, mBSC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 20496
VEGA: 18
Homologene: 20283
Rpa2
Name: replication protein A2
Synonyms: Rf-A2, RPA34, 30-kDa protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 19891
Homologene: 37712
Rttn
Name: rotatin
Synonyms: C530033I08Rik, 4921538A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 246102
VEGA: 18
Homologene: 65275
Trps1
Name: transcriptional repressor GATA binding 1
Synonyms: D15Ertd586e, trichorhinophalangeal syndrome I (human)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 83925
Homologene: 8556
Ptbp1
Name: polypyrimidine tract binding protein 1
Synonyms: Ptb, hnRNP I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 19205
HGNC: HGNC:9583
Homologene: 49188
Strn3
Name: striatin, calmodulin binding protein 3
Synonyms: SG2NA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 94186
VEGA: 12
Homologene: 82078
Aamp
Name: angio-associated migratory protein
Synonyms: Aamp-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 227290
HGNC: HGNC:18
Homologene: 846
Lrrc55
Name: leucine rich repeat containing 55
Synonyms: A330097E02Rik, LOC241528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241528
VEGA: 2
Homologene: 19385
Cyp4x1
Name: cytochrome P450, family 4, subfamily x, polypeptide 1
Synonyms: Cyp4a28-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 81906
Homologene: 75853
Itgb1
Name: integrin beta 1 (fibronectin receptor beta)
Synonyms: Gm9863, CD29, 4633401G24Rik, Fnrb, beta1 integrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 16412
HGNC: HGNC:6153
Homologene: 22999
Mfsd14b
Name: major facilitator superfamily domain containing 14B
Synonyms: 5730414C17Rik, Hiatl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 66631
Homologene: 64464
Slc38a9
Name: solute carrier family 38, member 9
Synonyms: 4833412L08Rik, 6720411P22Rik, 9130023D20Rik, 9430067K09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 268706
VEGA: 13
Homologene: 18139
2610008E11Rik
Name: RIKEN cDNA 2610008E11 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 72128
Homologene: 86705
Pnpla7
Name: patatin-like phospholipase domain containing 7
Synonyms: E430013P11Rik, NRE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241274
Homologene: 62431
Slc9a4
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 4
Synonyms: D730009J23Rik, NHE4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 110895
Homologene: 72232
Pak2
Name: p21 (RAC1) activated kinase 2
Synonyms: A130002K10Rik, D16Ertd269e, PAK-2, 5330420P17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 224105
HGNC: HGNC:8591
Homologene: 99711
Aif1l
Name: allograft inflammatory factor 1-like
Synonyms: 2810003C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 108897
Homologene: 12863
Epha3
Name: Eph receptor A3
Synonyms: Tyro4, End3, Hek, Hek4, Cek4, Mek4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13837
VEGA: 16
HGNC: HGNC:3387
Homologene: 21083
Kcp
Name: kielin/chordin-like protein
Synonyms: KCP, LOC333088, Crim2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 333088
Homologene: 87817
Efcab6
Name: EF-hand calcium binding domain 6
Synonyms: 4931407K02Rik, 4932408N08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 77627
Homologene: 11259
Atp1a3
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms: Atpa-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 232975
HGNC: HGNC:801
Homologene: 113729
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Ccdc116
Name: coiled-coil domain containing 116
Synonyms: 4930432J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 76872
Homologene: 51873
Dpysl4
Name: dihydropyrimidinase-like 4
Synonyms: Drp-4, Ulip4, CRMP-3, Crmp3, DPY4, unc-33-like phosphoprotein 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 26757
HGNC: HGNC:3016
Homologene: 4691
Xirp2
Name: xin actin-binding repeat containing 2
Synonyms: A530024P18Rik, myomaxin, Cmya3, 2310008C07Rik, mXin beta, 2310003D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241431
Homologene: 19388
Stard9
Name: START domain containing 9
Synonyms: E230025N21Rik, 4831403C07Rik, Kif16a, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 668880
Homologene: 130712
Esyt1
Name: extended synaptotagmin-like protein 1
Synonyms: Fam62a, Mbc2, vp115
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 23943
VEGA: 10
Homologene: 40794
Abcb1b
Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1B
Synonyms: Pgy-1, Mdr1, Abcb1, Mdr1b, mdr, Pgy1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18669
Homologene: 69084
Slc25a45
Name: solute carrier family 25, member 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 107375
Homologene: 76679
C130026I21Rik
Name: RIKEN cDNA C130026I21 gene
Synonyms: OTTMUSG00000029174
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 620078
Homologene: 131208
Krba1
Name: KRAB-A domain containing 1
Synonyms: A930040G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 77827
Homologene: 15880
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 243369
Homologene: 45453
Snx19
Name: sorting nexin 19
Synonyms: 3526401K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 102607
VEGA: 9
Homologene: 8846
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237711
Homologene: 104282
Acap1
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms: Centb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216859
Homologene: 22835
Rfc3
Name: replication factor C (activator 1) 3
Synonyms: 2810416I22Rik, Recc3, 38kDa, 38kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 69263
HGNC: HGNC:9971
Homologene: 2188
Pcolce
Name: procollagen C-endopeptidase enhancer protein
Synonyms: Astt2, Astt-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 18542
HGNC: HGNC:8738
Homologene: 1946
Galnt1
Name: polypeptide N-acetylgalactosaminyltransferase 1
Synonyms: ppGaNTase-T1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 14423
HGNC: HGNC:4123
Homologene: 8469
Nipsnap3a
Name: nipsnap homolog 3A
Synonyms: 1700054F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 73398
Homologene: 136268
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, EG637898, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 637898
Homologene: 129683
Plch1
Name: phospholipase C, eta 1
Synonyms: Plcl3, PLCeta1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 269437
Homologene: 88833
Sh2b2
Name: SH2B adaptor protein 2
Synonyms: Aps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 23921
Homologene: 10309
Bhmt-ps1
Name: betaine-homocysteine methyltransferase, pseudogene 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 57421
Trim43b
Name: tripartite motif-containing 43B
Synonyms: Gm8269
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 666747
Homologene: 105432
Phf1
Name: PHD finger protein 1
Synonyms: D17Ertd455e, mPcl1, PHF2, Tctex-3, Tctex3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 21652
VEGA: 17
HGNC: HGNC:8919
Homologene: 7567
Glis1
Name: GLIS family zinc finger 1
Synonyms: GliH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230587
Homologene: 77390
Gm15854
Name: predicted gene 15854
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 667764
Homologene: 110821
Ndufs2
Name: NADH:ubiquinone oxidoreductase core subunit S2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 226646
HGNC: HGNC:7708
Homologene: 56659
Ly6i
Name: lymphocyte antigen 6 complex, locus I
Synonyms: Ly-6I, Ly-6M
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 57248
Homologene: 113718
Plekha2
Name: pleckstrin homology domain-containing, family A (phosphoinositide binding specific) member 2
Synonyms: 6430512N22Rik, TAPP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 83436
Homologene: 41822
Wdr81
Name: WD repeat domain 81
Synonyms: shakey 5, MGC32441, nur5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192652
Homologene: 13983
Vmn1r220
Name: vomeronasal 1 receptor 220
Synonyms: V1rh12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 171271
Homologene: 110880
Phc3
Name: polyhomeotic 3
Synonyms: HPH3, EDR3, E030046K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 241915
Homologene: 69390
Cyb5rl
Name: cytochrome b5 reductase-like
Synonyms: 2810410C14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230582
Homologene: 45506
Oxgr1
Name: oxoglutarate (alpha-ketoglutarate) receptor 1
Synonyms: P2Y15, Gpr99, Gpr80, LOC239283, Cysltr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 239283
VEGA: 14
HGNC: HGNC:4531
Homologene: 25878
Olfr1288
Name: olfactory receptor 1288
Synonyms: MOR245-9, GA_x6K02T2Q125-72530279-72531217
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258395
Homologene: 88360
G730046D07Rik
Name: RIKEN cDNA G730046D07 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
Gm6189
Name: predicted gene 6189
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 620899
Pcdhgb7
Name: protocadherin gamma subfamily B, 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 93704
HGNC: HGNC:8714
Homologene: 75102
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 40,619,126 bp
  • A to T, chromosome 1 at 74,281,126 bp
  • T to C, chromosome 1 at 80,383,519 bp
  • T to C, chromosome 1 at 85,112,557 bp
  • A to G, chromosome 1 at 171,239,293 bp
  • T to C, chromosome 2 at 24,982,114 bp
  • A to T, chromosome 2 at 31,962,239 bp
  • A to T, chromosome 2 at 67,516,493 bp
  • G to A, chromosome 2 at 85,196,116 bp
  • A to T, chromosome 2 at 111,479,144 bp
  • A to G, chromosome 2 at 120,664,991 bp
  • A to T, chromosome 3 at 30,936,819 bp
  • G to A, chromosome 3 at 63,701,900 bp
  • T to A, chromosome 4 at 26,369,352 bp
  • T to A, chromosome 4 at 52,997,251 bp
  • T to C, chromosome 4 at 107,080,988 bp
  • T to C, chromosome 4 at 107,567,845 bp
  • A to T, chromosome 4 at 115,112,880 bp
  • T to G, chromosome 4 at 132,776,744 bp
  • T to A, chromosome 5 at 8,813,722 bp
  • G to A, chromosome 5 at 136,219,053 bp
  • T to A, chromosome 5 at 137,605,127 bp
  • C to A, chromosome 5 at 151,651,172 bp
  • A to G, chromosome 6 at 29,482,258 bp
  • G to A, chromosome 6 at 48,402,997 bp
  • G to T, chromosome 6 at 48,451,157 bp
  • A to T, chromosome 6 at 129,972,463 bp
  • T to C, chromosome 7 at 25,000,930 bp
  • A to T, chromosome 7 at 42,116,528 bp
  • C to G, chromosome 7 at 126,566,299 bp
  • A to G, chromosome 7 at 139,098,496 bp
  • T to C, chromosome 7 at 139,558,905 bp
  • A to G, chromosome 8 at 25,043,020 bp
  • C to A, chromosome 8 at 95,109,982 bp
  • T to G, chromosome 8 at 128,714,143 bp
  • T to G, chromosome 9 at 30,428,448 bp
  • T to A, chromosome 9 at 57,414,715 bp
  • T to A, chromosome 9 at 89,090,639 bp
  • A to G, chromosome 10 at 79,094,452 bp
  • T to C, chromosome 10 at 79,859,213 bp
  • T to C, chromosome 10 at 128,520,648 bp
  • G to A, chromosome 11 at 29,706,856 bp
  • T to C, chromosome 11 at 29,751,261 bp
  • A to G, chromosome 11 at 69,883,685 bp
  • T to C, chromosome 11 at 75,448,002 bp
  • C to T, chromosome 12 at 51,627,855 bp
  • T to A, chromosome 13 at 23,183,978 bp
  • C to T, chromosome 13 at 65,066,608 bp
  • G to A, chromosome 13 at 112,714,248 bp
  • T to A, chromosome 14 at 54,937,919 bp
  • T to C, chromosome 14 at 120,022,613 bp
  • A to G, chromosome 15 at 50,846,648 bp
  • A to T, chromosome 15 at 74,983,035 bp
  • C to A, chromosome 15 at 83,867,108 bp
  • A to G, chromosome 16 at 17,146,945 bp
  • G to T, chromosome 16 at 32,052,210 bp
  • G to A, chromosome 16 at 63,583,539 bp
  • C to T, chromosome 17 at 26,937,500 bp
  • T to C, chromosome 18 at 12,450,403 bp
  • T to C, chromosome 18 at 24,238,356 bp
  • T to A, chromosome 18 at 37,753,803 bp
  • T to A, chromosome 18 at 57,910,256 bp
  • T to C, chromosome 18 at 89,095,584 bp
  • A to T, chromosome 19 at 5,880,118 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4223 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041043-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.