Strain Name:
C57BL/6J-MtgxR4224Btlr/Mmmh
Stock Number:
041044-MU
Citation ID:
RRID:MMRRC_041044-MU
Other Names:
R4224 (G1), C57BL/6J-MtgxR4224Btlr
Major Collection:

Gene Information

Abl2
Name: v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene)
Synonyms: Arg, Abll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Ptbp1
Name: polypyrimidine tract binding protein 1
Synonyms: hnRNP I, Ptb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19205
HGNC: HGNC:9583
Homologene: 49188
Polr1has
Name: RNA polymerase I subunit H, antisense
Synonyms: 1700022C21Rik, Znrd1as
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76416
Homologene: 130062
Atp6v1a
Name: ATPase, H+ transporting, lysosomal V1 subunit A
Synonyms: VPP2, VA68, Atp6a1, lysosomal 70kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11964
HGNC: HGNC:851
Homologene: 123934
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 211652
Homologene: 69180
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Gm7964
Name: predicted gene 7964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666170
Trim37
Name: tripartite motif-containing 37
Synonyms: MUL, TEF3, 2810004E07Rik, 1110032A10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68729
HGNC: HGNC:7523
Homologene: 9084
Khdc3
Name: KH domain containing 3, subcortical maternal complex member
Synonyms: ecat1, FILIA, 2410004A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66991
Homologene: 49842
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, Ip3r3, tf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Tnnt1
Name: troponin T1, skeletal, slow
Synonyms: Tnt, skeletal muscle slow-twitch TnT, sTnT, ssTnT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21955
Homologene: 20704
Aif1l
Name: allograft inflammatory factor 1-like
Synonyms: 2810003C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 108897
Homologene: 12863
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b1203Clo, b2b1279Clo, b2b598Clo, b2b1289Clo, b2b1727Clo, Dnahc11, avc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 672511
Homologene: 45439
Vmn1r73
Name: vomeronasal 1 receptor 73
Synonyms: V1rg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171237
Homologene: 103842
Stard9
Name: START domain containing 9
Synonyms: N-3 kinesin, Kif16a, 4831403C07Rik, E230025N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Fer1l4
Name: fer-1-like 4 (C. elegans)
Synonyms: 9130402C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74562
Homologene: 19075
Fam214a
Name: family with sequence similarity 214, member A
Synonyms: 6330415I01Rik, C130047D21Rik, BC031353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235493
VEGA: 9
Homologene: 35065
Slc8a1
Name: solute carrier family 8 (sodium/calcium exchanger), member 1
Synonyms: Ncx1, D930008O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20541
VEGA: 17
Homologene: 69090
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53419
Homologene: 4804
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243369
Homologene: 45453
Ica1
Name: islet cell autoantigen 1
Synonyms: ICA69, 69kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15893
HGNC: HGNC:5343
Homologene: 7777
Olfr862
Name: olfactory receptor 862
Synonyms: GA_x6K02T2PVTD-13624132-13623212, MOR146-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258555
Homologene: 115540
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: 2900083P10Rik, C230094A16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237711
Homologene: 104282
Spdya
Name: speedy/RINGO cell cycle regulator family, member A
Synonyms: 4930548B21Rik, 4921517J08Rik, Spdy1, speedy/ringo, speedy A2, speedy A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70891
Homologene: 16357
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, EG637898, Gprc2a-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 637898
Homologene: 129683
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231591
Homologene: 129606
Acsbg1
Name: acyl-CoA synthetase bubblegum family member 1
Synonyms: E230019G03Rik, BG1, Lpd, Bgm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 94180
Homologene: 9044
Trim43b
Name: tripartite motif-containing 43B
Synonyms: Gm8269
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 666747
Homologene: 105432
Olfr617
Name: olfactory receptor 617
Synonyms: GA_x6K02T2PBJ9-6306819-6307775, MOR31-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258838
Homologene: 105208
Mr1
Name: major histocompatibility complex, class I-related
Synonyms: MR1, H2ls
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15064
HGNC: HGNC:4975
Homologene: 123981
Ubash3a
Name: ubiquitin associated and SH3 domain containing, A
Synonyms: 5830413C03Rik, Sts-2, TULA
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328795
Homologene: 56833
Cmc2
Name: COX assembly mitochondrial protein 2
Synonyms: 1110046L09Rik, 2310061C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66531
Homologene: 41350
Pals1
Name: protein associated with LIN7 1, MAGUK family member
Synonyms: Pals1, 3830420B02Rik, Mpp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56217
VEGA: 12
Homologene: 9512
Mettl21a
Name: methyltransferase like 21A
Synonyms: 2310038H17Rik, Fam119a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67099
Homologene: 41658
Grin1os
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1), opposite strand
Synonyms: E130003G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320354
Pcdhgb7
Name: protocadherin gamma subfamily B, 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93704
HGNC: HGNC:8714
Homologene: 75102
Tssk3
Name: testis-specific serine kinase 3
Synonyms: Tssk-3, 4930594I21Rik, Stk22d, Stk22c, 1700014N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 58864
Homologene: 62163
9330182O14Rik
Name: RIKEN cDNA 9330182O14 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 328531
VEGA: 15
Gm15946
Name: predicted gene 15946
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 108168333
Zcchc18
Name: zinc finger, CCHC domain containing 18
Synonyms: 1500031H04Rik, Sizn2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 66995
Homologene: 121722
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,607,956 bp
  • A to T, chromosome 1 at 155,130,719 bp
  • A to G, chromosome 1 at 156,633,847 bp
  • T to C, chromosome 2 at 25,297,320 bp
  • A to T, chromosome 2 at 31,962,239 bp
  • A to G, chromosome 2 at 120,664,991 bp
  • A to C, chromosome 2 at 156,020,389 bp
  • T to C, chromosome 4 at 129,490,599 bp
  • T to C, chromosome 5 at 72,343,108 bp
  • A to G, chromosome 5 at 109,216,283 bp
  • T to C, chromosome 6 at 8,659,960 bp
  • G to T, chromosome 6 at 48,451,157 bp
  • T to C, chromosome 7 at 4,510,007 bp
  • T to C, chromosome 7 at 11,756,579 bp
  • A to T, chromosome 7 at 42,116,528 bp
  • T to G, chromosome 7 at 83,756,822 bp
  • A to G, chromosome 7 at 103,584,759 bp
  • G to A, chromosome 8 at 116,894,127 bp
  • T to A, chromosome 9 at 19,883,600 bp
  • C to T, chromosome 9 at 54,616,041 bp
  • C to G, chromosome 9 at 73,102,871 bp
  • T to A, chromosome 9 at 75,008,726 bp
  • T to A, chromosome 9 at 89,090,639 bp
  • T to C, chromosome 10 at 79,859,213 bp
  • T to C, chromosome 11 at 29,751,261 bp
  • G to A, chromosome 11 at 35,870,527 bp
  • T to A, chromosome 11 at 87,216,463 bp
  • C to T, chromosome 11 at 119,436,823 bp
  • A to G, chromosome 12 at 78,829,718 bp
  • T to G, chromosome 12 at 118,130,892 bp
  • A to G, chromosome 15 at 35,876,419 bp
  • T to C, chromosome 15 at 40,142,276 bp
  • C to A, chromosome 16 at 44,101,811 bp
  • T to C, chromosome 17 at 12,962,885 bp
  • T to C, chromosome 17 at 27,107,258 bp
  • T to C, chromosome 17 at 31,237,928 bp
  • T to C, chromosome 17 at 36,958,725 bp
  • G to A, chromosome 17 at 71,562,524 bp
  • A to G, chromosome 17 at 81,649,352 bp
  • T to C, chromosome 18 at 12,450,403 bp
  • T to A, chromosome 18 at 37,753,803 bp
  • A to T, chromosome X at 136,994,666 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041044-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.