Strain Name:
C57BL/6J-MtgxR4224Btlr/Mmmh
Stock Number:
041044-MU
Citation ID:
RRID:MMRRC_041044-MU
Other Names:
R4224 (G1), C57BL/6J-MtgxR4224Btlr
Major Collection:

Strain Information

Abl2
Name: ABL proto-oncogene 2, non-receptor tyrosine kinase
Synonyms: Arg, Abll
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Ptbp1
Name: polypyrimidine tract binding protein 1
Synonyms: hnRNP I, Ptb
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19205
HGNC: HGNC:9583
Homologene: 49188
Polr1has
Name: RNA polymerase I subunit H, antisense
Synonyms: Znrd1as, 1700022C21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76416
Homologene: 130062
Atp6v1a
Name: ATPase, H+ transporting, lysosomal V1 subunit A
Synonyms: lysosomal 70kDa, VPP2, VA68, Atp6a1
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 11964
HGNC: HGNC:851
Homologene: 123934
Wwc1
Name: WW, C2 and coiled-coil domain containing 1
Synonyms: Kibra
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 211652
Homologene: 69180
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, C330002D13Rik, Coh1, 2310042E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Gm7964
Name: predicted gene 7964
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 666170
Trim37
Name: tripartite motif-containing 37
Synonyms: TEF3, 2810004E07Rik, MUL, 1110032A10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68729
HGNC: HGNC:7523
Homologene: 9084
Khdc3
Name: KH domain containing 3, subcortical maternal complex member
Synonyms: 2410004A20Rik, FILIA, ecat1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66991
Homologene: 49842
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: tf, Itpr-3, Ip3r3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Tnnt1
Name: troponin T1, skeletal, slow
Synonyms: sTnT, ssTnT, skeletal muscle slow-twitch TnT, Tnt
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 21955
Homologene: 20704
Aif1l
Name: allograft inflammatory factor 1-like
Synonyms: 2810003C17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 108897
Homologene: 12863
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1203Clo, b2b1289Clo, lrd, avc4, Dnahc11, b2b1279Clo, b2b598Clo, b2b1727Clo
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Lama3
Name: laminin, alpha 3
Synonyms: [a]3B, nicein, 150kDa
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Rnf213
Name: ring finger protein 213
Synonyms: D11Ertd759e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 672511
Homologene: 45439
Vmn1r73
Name: vomeronasal 1 receptor 73
Synonyms: V1rg2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 171237
Homologene: 103842
Stard9
Name: StAR related lipid transfer domain containing 9
Synonyms: N-3 kinesin, E230025N21Rik, Kif16a, 4831403C07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 668880
Homologene: 130712
Fer1l4
Name: fer-1 like family member 4
Synonyms: 9130402C12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 74562
Homologene: 19075
Atosa
Name: atos homolog A
Synonyms: BC031353, Fam214a, C130047D21Rik, 6330415I01Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235493
VEGA: 9
Homologene: 35065
Slc8a1
Name: solute carrier family 8 (sodium/calcium exchanger), member 1
Synonyms: Ncx1, D930008O12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20541
VEGA: 17
Homologene: 69090
Corin
Name: corin, serine peptidase
Synonyms: Lrp4
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 53419
Homologene: 4804
Sspo
Name: SCO-spondin
Synonyms: C79529, Scospondin
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243369
Homologene: 45453
Ica1
Name: islet cell autoantigen 1
Synonyms: ICA69, 69kDa
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15893
HGNC: HGNC:5343
Homologene: 7777
Or7e170
Name: olfactory receptor family 7 subfamily E member 170
Synonyms: GA_x6K02T2PVTD-13624132-13623212, MOR146-1, Olfr862
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258555
Homologene: 115540
Eml6
Name: echinoderm microtubule associated protein like 6
Synonyms: C230094A16Rik, 2900083P10Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237711
Homologene: 104282
Spdya
Name: speedy/RINGO cell cycle regulator family, member A
Synonyms: 4930548B21Rik, speedy A2, 4921517J08Rik, Spdy1, speedy/ringo, speedy A1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70891
Homologene: 16357
Vmn2r60
Name: vomeronasal 2, receptor 60
Synonyms: Casr-rs3, Gprc2a-rs3, EG637898
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 637898
Homologene: 129683
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231591
Homologene: 129606
Acsbg1
Name: acyl-CoA synthetase bubblegum family member 1
Synonyms: Bgm, BG1, Lpd, E230019G03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 94180
Homologene: 9044
Or52z12
Name: olfactory receptor family 52 subfamily Z member 12
Synonyms: Olfr617, MOR31-10, GA_x6K02T2PBJ9-6306819-6307775
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258838
Homologene: 105208
Mr1
Name: major histocompatibility complex, class I-related
Synonyms: MR1, H2ls
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 15064
HGNC: HGNC:4975
Homologene: 123981
Ubash3a
Name: ubiquitin associated and SH3 domain containing, A
Synonyms: 5830413C03Rik, Sts-2, TULA
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328795
Homologene: 56833
Cmc2
Name: C-X9-C motif containing 2
Synonyms: 2310061C15Rik, 1110046L09Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 66531
Homologene: 41350
Pals1
Name: protein associated with LIN7 1, MAGUK family member
Synonyms: Mpp5, Pals1, 3830420B02Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 56217
VEGA: 12
Homologene: 9512
Mettl21a
Name: methyltransferase 21A, HSPA lysine
Synonyms: 2310038H17Rik, Fam119a
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67099
Homologene: 41658
Grin1os
Name: glutamate receptor, ionotropic, NMDA1 (zeta 1), opposite strand
Synonyms: E130003G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320354
Pcdhgb7
Name: protocadherin gamma subfamily B, 7
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93704
HGNC: HGNC:8714
Homologene: 75102
Tssk3
Name: testis-specific serine kinase 3
Synonyms: Stk22c, Stk22d, Tssk-3, 4930594I21Rik, 1700014N07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 58864
Homologene: 62163
9330182O14Rik
Name: RIKEN cDNA 9330182O14 gene
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328531
VEGA: 15
Gm15946
Name: predicted gene 15946
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 108168333
Zcchc18
Name: zinc finger, CCHC domain containing 18
Synonyms: Sizn2, 1500031H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 66995
Homologene: 121722
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 64,607,956 bp
  • A to T, chromosome 1 at 155,130,719 bp
  • A to G, chromosome 1 at 156,633,847 bp
  • T to C, chromosome 2 at 25,297,320 bp
  • A to T, chromosome 2 at 31,962,239 bp
  • A to G, chromosome 2 at 120,664,991 bp
  • A to C, chromosome 2 at 156,020,389 bp
  • T to C, chromosome 4 at 129,490,599 bp
  • T to C, chromosome 5 at 72,343,108 bp
  • A to G, chromosome 5 at 109,216,283 bp
  • T to C, chromosome 6 at 8,659,960 bp
  • G to T, chromosome 6 at 48,451,157 bp
  • T to C, chromosome 7 at 4,510,007 bp
  • T to C, chromosome 7 at 11,756,579 bp
  • A to T, chromosome 7 at 42,116,528 bp
  • T to G, chromosome 7 at 83,756,822 bp
  • A to G, chromosome 7 at 103,584,759 bp
  • G to A, chromosome 8 at 116,894,127 bp
  • T to A, chromosome 9 at 19,883,600 bp
  • C to T, chromosome 9 at 54,616,041 bp
  • C to G, chromosome 9 at 73,102,871 bp
  • T to A, chromosome 9 at 75,008,726 bp
  • T to A, chromosome 9 at 89,090,639 bp
  • T to C, chromosome 10 at 79,859,213 bp
  • T to C, chromosome 11 at 29,751,261 bp
  • G to A, chromosome 11 at 35,870,527 bp
  • T to A, chromosome 11 at 87,216,463 bp
  • C to T, chromosome 11 at 119,436,823 bp
  • A to G, chromosome 12 at 78,829,718 bp
  • T to G, chromosome 12 at 118,130,892 bp
  • A to G, chromosome 15 at 35,876,419 bp
  • T to C, chromosome 15 at 40,142,276 bp
  • C to A, chromosome 16 at 44,101,811 bp
  • T to C, chromosome 17 at 12,962,885 bp
  • T to C, chromosome 17 at 27,107,258 bp
  • T to C, chromosome 17 at 31,237,928 bp
  • T to C, chromosome 17 at 36,958,725 bp
  • G to A, chromosome 17 at 71,562,524 bp
  • A to G, chromosome 17 at 81,649,352 bp
  • T to C, chromosome 18 at 12,450,403 bp
  • T to A, chromosome 18 at 37,753,803 bp
  • A to T, chromosome X at 136,994,666 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4224 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041044-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.