Strain Name:
C57BL/6J-MtgxR4247Btlr/Mmmh
Stock Number:
041063-MU
Citation ID:
RRID:MMRRC_041063-MU
Other Names:
R4247 (G1), C57BL/6J-MtgxR4247Btlr
Major Collection:

Gene Information

Upp1
Name: uridine phosphorylase 1
Synonyms: UdRPase, UPase, Up
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 22271
Homologene: 2524
Resf1
Name: retroelement silencing factor 1
Synonyms: 2810474O19Rik, GET
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67246
Homologene: 19251
Ranbp2
Name: RAN binding protein 2
Synonyms: A430087B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 19386
VEGA: 10
Homologene: 87808
Snx8
Name: sorting nexin 8
Synonyms: B130023O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231834
Homologene: 8338
Slitrk6
Name: SLIT and NTRK-like family, member 6
Synonyms: 4832410J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 239250
VEGA: 14
Homologene: 12986
Fubp1
Name: far upstream element (FUSE) binding protein 1
Synonyms: FBP, Fubp4, Fubp, 9530027K12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 51886
HGNC: HGNC:4004
Homologene: 48253
Sumf1
Name: sulfatase modifying factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 58911
Homologene: 16268
Erap1
Name: endoplasmic reticulum aminopeptidase 1
Synonyms: Arts1, PILSAP, ERAAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 80898
VEGA: 13
Homologene: 56754
Wrnip1
Name: Werner helicase interacting protein 1
Synonyms: 4833444L21Rik, WHIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 78903
Homologene: 10592
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 171395
Homologene: 51376
Slitrk1
Name: SLIT and NTRK-like family, member 1
Synonyms: 3200001I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 76965
VEGA: 14
Homologene: 14174
Trp73
Name: transformation related protein 73
Synonyms: deltaNp73, TAp73, p73
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 22062
Homologene: 3960
Kif27
Name: kinesin family member 27
Synonyms: 4930517I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 75050
VEGA: 13
Homologene: 69235
Zfyve9
Name: zinc finger, FYVE domain containing 9
Synonyms: Madhip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230597
HGNC: HGNC:6775
Homologene: 3527
Grasp
Name: GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein
Synonyms: tamalin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 56149
VEGA: 15
Homologene: 32442
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: mSNM1B, Apollo, SNMIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 140917
Homologene: 32553
Kif9
Name: kinesin family member 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 16578
Homologene: 65620
Akna
Name: AT-hook transcription factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100182
Homologene: 49947
Ccdc97
Name: coiled-coil domain containing 97
Synonyms: 1200014H14Rik, 2810446P04Rik, D7Ertd462e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 52132
Homologene: 32701
Gtf3c5
Name: general transcription factor IIIC, polypeptide 5
Synonyms: TFIIICepsilon, TFIIIC63, 2700084A09Rik, TFiiiC2-63
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70239
HGNC: HGNC:4668
Homologene: 40806
Hecw2
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Synonyms: Nedl2, D030049F17Rik, A730039N16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329152
Homologene: 66192
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 620928
Homologene: 115466
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 381293
Homologene: 8916
Adam22
Name: a disintegrin and metallopeptidase domain 22
Synonyms: 2900022I03Rik, MDC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11496
HGNC: HGNC:201
Homologene: 37898
Olfr690
Name: olfactory receptor 690
Synonyms: MOR31-2, GA_x6K02T2PBJ9-7959171-7958224
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 56860
Homologene: 10653
Atp8a1
Name: ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
Synonyms: Atp3a2, B230107D19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 11980
Homologene: 48402
Hsdl2
Name: hydroxysteroid dehydrogenase like 2
Synonyms: 2610207I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 72479
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: NaT, NaN, SNS2, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 24046
VEGA: 9
Homologene: 8041
Lrrc24
Name: leucine rich repeat containing 24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 378937
Homologene: 86785
Zfp945
Name: zinc finger protein 945
Synonyms: A630033E08Rik, C730040L01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 240041
Homologene: 133214
Lmtk3
Name: lemur tyrosine kinase 3
Synonyms: Aatyk3, AATYK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 381983
Homologene: 79449
Vmn2r97
Name: vomeronasal 2, receptor 97
Synonyms: EG627367
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 627367
Homologene: 115024
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 74772
Homologene: 56940
Aga
Name: aspartylglucosaminidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11593
HGNC: HGNC:318
Homologene: 13
AA792892
Name: expressed sequence AA792892
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 100554
Homologene: 133359
Gm20715
Name: predicted gene 20715
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
Satl1
Name: spermidine/spermine N1-acetyl transferase-like 1
Synonyms: 4930404K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 73809
Homologene: 28332
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 53,832,645 bp
  • G to T, chromosome 1 at 136,473,388 bp
  • T to C, chromosome 2 at 28,571,184 bp
  • A to C, chromosome 3 at 103,804,084 bp
  • G to A, chromosome 3 at 152,231,936 bp
  • A to T, chromosome 4 at 59,594,417 bp
  • C to T, chromosome 4 at 63,395,172 bp
  • C to A, chromosome 4 at 108,719,192 bp
  • T to C, chromosome 4 at 140,992,228 bp
  • A to G, chromosome 4 at 154,064,632 bp
  • T to C, chromosome 5 at 8,145,626 bp
  • C to T, chromosome 5 at 67,667,574 bp
  • A to G, chromosome 5 at 94,383,587 bp
  • A to G, chromosome 5 at 140,356,045 bp
  • A to C, chromosome 6 at 108,155,013 bp
  • T to A, chromosome 6 at 149,325,543 bp
  • A to G, chromosome 7 at 6,438,902 bp
  • T to G, chromosome 7 at 25,716,034 bp
  • G to A, chromosome 7 at 45,794,062 bp
  • A to T, chromosome 7 at 85,872,675 bp
  • T to C, chromosome 7 at 105,330,148 bp
  • T to A, chromosome 8 at 53,511,830 bp
  • G to T, chromosome 9 at 110,495,959 bp
  • C to T, chromosome 9 at 119,807,886 bp
  • A to G, chromosome 10 at 58,478,864 bp
  • C to T, chromosome 11 at 8,865,543 bp
  • T to C, chromosome 11 at 9,134,815 bp
  • T to C, chromosome 13 at 32,806,883 bp
  • A to G, chromosome 13 at 58,287,917 bp
  • T to C, chromosome 13 at 74,675,295 bp
  • A to G, chromosome 14 at 108,912,562 bp
  • T to A, chromosome 14 at 110,750,739 bp
  • A to G, chromosome 15 at 76,717,976 bp
  • G to T, chromosome 15 at 101,224,537 bp
  • T to C, chromosome 17 at 18,947,280 bp
  • C to T, chromosome 17 at 22,850,609 bp
  • A to G, chromosome X at 112,406,336 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4247 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041063-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.