Strain Name:
C57BL/6J-MtgxR4250Btlr/Mmmh
Stock Number:
041066-MU
Citation ID:
RRID:MMRRC_041066-MU
Other Names:
R4250 (G1), C57BL/6J-MtgxR4250Btlr
Major Collection:

Strain Information

Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319582
Homologene: 35306
Colgalt2
Name: collagen beta(1-O)galactosyltransferase 2
Synonyms: Glt25d2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 269132
Homologene: 22865
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Birc2
Name: baculoviral IAP repeat-containing 2
Synonyms: HIAP1, MIHB, IAP1, mcIAP1, Api1, cIAP-1, MIAP1, cIAP1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 11797
HGNC: HGNC:590
Homologene: 900
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 4930431J08Rik, 9330107J05Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Sirt1
Name: sirtuin 1
Synonyms: Sir2alpha, Sir2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 93759
Homologene: 56556
Yeats2
Name: YEATS domain containing 2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 208146
VEGA: 16
Homologene: 9967
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Ezr
Name: ezrin
Synonyms: Vil2, ezrin, cytovillin, p81
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22350
Homologene: 55740
Snx8
Name: sorting nexin 8
Synonyms: B130023O14Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231834
Homologene: 8338
Chst3
Name: carbohydrate sulfotransferase 3
Synonyms: C6ST-1, GST-0, C6ST
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53374
HGNC: HGNC:1971
Homologene: 3156
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik, sao
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 68750
Homologene: 2218
Ikzf1
Name: IKAROS family zinc finger 1
Synonyms: Ikaros, LyF-1, 5832432G11Rik, Zfpn1a1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 22778
Homologene: 55948
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171395
Homologene: 51376
Ttn
Name: titin
Synonyms: connectin, shru, D330041I19Rik, 2310057K23Rik, mdm, D830007G01Rik, 2310074I15Rik, 1100001C23Rik, 2310036G12Rik, L56
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dclre1b
Name: DNA cross-link repair 1B
Synonyms: Apollo, SNMIB, mSNM1B
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 140917
Homologene: 32553
Pilra
Name: paired immunoglobin-like type 2 receptor alpha
Synonyms: FDF03
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231805
Homologene: 8387
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Kif14
Name: kinesin family member 14
Synonyms: D1Ertd367e, N-3 kinesin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381293
Homologene: 8916
Padi2
Name: peptidyl arginine deiminase, type II
Synonyms: Pdi, Pdi2, PAD type II
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18600
Homologene: 7214
Rxfp1
Name: relaxin/insulin-like family peptide receptor 1
Synonyms: LOC381489, Lgr7
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 381489
Homologene: 11007
Sp7
Name: Sp7 transcription factor 7
Synonyms: osterix, 6430578P22Rik, Osx
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 170574
VEGA: 15
Homologene: 15607
Hoxb5
Name: homeobox B5
Synonyms: Hox-2.1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15413
HGNC: HGNC:5116
Homologene: 37585
Or5b97
Name: olfactory receptor family 5 subfamily B member 97
Synonyms: GA_x6K02T2RE5P-3231251-3230331, MOR202-3, Olfr1447
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258698
HGNC: HGNC:8324
Homologene: 74233
Tcte1
Name: t-complex-associated testis expressed 1
Synonyms: D17Sil1, Tcte-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 21645
VEGA: 17
Homologene: 8434
Ttc4
Name: tetratricopeptide repeat domain 4
Synonyms: L62, 2810002P21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 72354
Homologene: 31249
Lmtk3
Name: lemur tyrosine kinase 3
Synonyms: AATYK3, Aatyk3
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381983
Homologene: 79449
Bcl2a1d
Name: B cell leukemia/lymphoma 2 related protein A1d
Synonyms: A1-d
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12047
HGNC: HGNC:991
Homologene: 2988
Slc5a6
Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 330064
Homologene: 23277
Vmn2r-ps36
Name: vomeronasal 2, receptor, pseudogene 36
Synonyms: EG665442
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 665442
Hba-x
Name: hemoglobin X, alpha-like embryonic chain in Hba complex
Synonyms: zeta globin
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 15126
HGNC: HGNC:4835
Homologene: 110705
Igkv8-26
Name: immunoglobulin kappa variable 8-26
Synonyms: Gm16945
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 620240
HGNC: HGNC:5834
Gm26592
Name: predicted gene, 26592
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Satl1
Name: spermidine/spermine N1-acetyl transferase-like 1
Synonyms: 4930404K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 73809
Homologene: 28332
Vmn2r-ps41
Name: vomeronasal 2, receptor, pseudogene 41
Synonyms: Gm6845
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 628128
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 24,525,645 bp
  • T to C, chromosome 1 at 63,302,861 bp
  • G to T, chromosome 1 at 136,473,388 bp
  • A to T, chromosome 1 at 152,489,887 bp
  • T to C, chromosome 2 at 76,713,712 bp
  • A to G, chromosome 3 at 79,652,272 bp
  • A to C, chromosome 3 at 103,804,084 bp
  • G to A, chromosome 4 at 106,665,683 bp
  • A to G, chromosome 4 at 140,906,546 bp
  • A to G, chromosome 5 at 31,037,718 bp
  • A to T, chromosome 5 at 137,823,552 bp
  • A to G, chromosome 5 at 140,356,045 bp
  • T to G, chromosome 5 at 150,310,360 bp
  • T to A, chromosome 6 at 58,916,516 bp
  • G to A, chromosome 6 at 70,193,246 bp
  • T to A, chromosome 7 at 7,417,786 bp
  • T to A, chromosome 7 at 9,177,250 bp
  • G to A, chromosome 7 at 45,794,062 bp
  • C to A, chromosome 8 at 36,512,212 bp
  • A to T, chromosome 9 at 7,818,935 bp
  • A to G, chromosome 9 at 21,037,739 bp
  • C to T, chromosome 9 at 88,731,653 bp
  • A to T, chromosome 10 at 60,186,068 bp
  • T to A, chromosome 10 at 63,337,098 bp
  • C to T, chromosome 11 at 8,865,543 bp
  • C to T, chromosome 11 at 11,754,166 bp
  • T to C, chromosome 11 at 32,278,000 bp
  • T to C, chromosome 11 at 96,304,028 bp
  • T to C, chromosome 13 at 37,893,893 bp
  • T to A, chromosome 15 at 102,358,892 bp
  • A to G, chromosome 16 at 20,156,935 bp
  • A to T, chromosome 17 at 6,754,797 bp
  • A to G, chromosome 17 at 45,539,691 bp
  • T to C, chromosome 19 at 12,901,004 bp
  • A to G, chromosome X at 112,406,336 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4250 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041066-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.