Strain Name:
C57BL/6J-MtgxR4254Btlr/Mmmh
Stock Number:
041067-MU
Citation ID:
RRID:MMRRC_041067-MU
Other Names:
R4254 (G1), C57BL/6J-MtgxR4254Btlr
Major Collection:

Gene Information

Sf1
Name: splicing factor 1
Synonyms: WBP4, MZFM, CW17R, Zfp162
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 22668
Homologene: 134065
Adgrg1
Name: adhesion G protein-coupled receptor G1
Synonyms: Cyt28, Gpr56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14766
HGNC: HGNC:4512
Homologene: 4156
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: EHSH1, Sh3p17, Ese1, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Serinc3
Name: serine incorporator 3
Synonyms: TMS-1, DIFF33, AIGP1, Tde1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26943
Homologene: 38230
Ipo11
Name: importin 11
Synonyms: E330021B14Rik, 1700081H05Rik, 2510001A17Rik, Ranbp11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76582
Homologene: 7089
Tle1
Name: transducin-like enhancer of split 1
Synonyms: Grg1, Estm14, C230057C06Rik, Tle4l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 21885
Homologene: 21058
Anapc2
Name: anaphase promoting complex subunit 2
Synonyms: expressed during mesenchymal induction 4, Emi4, APC2, 9230107K09Rik, Imi4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99152
Homologene: 8359
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Dsg4
Name: desmoglein 4
Synonyms: lah, CDHF13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 16769
Homologene: 65341
Atp5g3
Name: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228033
HGNC: HGNC:843
Homologene: 133873
Cdh19
Name: cadherin 19, type 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227485
HGNC: HGNC:1758
Homologene: 23286
Asxl3
Name: ASXL transcriptional regulator 3
Synonyms: LOC381127, D930044O18Rik, D430002O22Rik, C230079D11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 211961
Homologene: 19371
Dnah5
Name: dynein, axonemal, heavy chain 5
Synonyms: Mdnah5, b2b1537Clo, b2b1565Clo, b2b1154Clo, b2b1134Clo, Dnahc5, b2b3491Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 110082
HGNC: HGNC:2950
Homologene: 1048
Depdc1a
Name: DEP domain containing 1a
Synonyms: 5830484J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76131
Homologene: 9834
Or8b101
Name: olfactory receptor family 8 subfamily B member 101
Synonyms: GA_x6K02T2PVTD-31787920-31788864, MOR162-4, Olfr888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258416
Homologene: 84577
Itgb2l
Name: integrin beta 2-like
Synonyms: pactolus, 5033406G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16415
HGNC: HGNC:6155
Nlrp1a
Name: NLR family, pyrin domain containing 1A
Synonyms: Nalp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 195046
Homologene: 133820
Slc16a10
Name: solute carrier family 16 (monocarboxylic acid transporters), member 10
Synonyms: PRO0813, TAT1, 2610103N14Rik, Mct10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 72472
VEGA: 10
Homologene: 75089
Slc15a2
Name: solute carrier family 15 (H+/peptide transporter), member 2
Synonyms: Pept2, 8430408C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 57738
Homologene: 56912
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241116
Homologene: 28093
Ccdc27
Name: coiled-coil domain containing 27
Synonyms: LOC381580
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 381580
Homologene: 17596
Tmbim1
Name: transmembrane BAX inhibitor motif containing 1
Synonyms: 2310061B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69660
Homologene: 36404
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Kcnb1
Name: potassium voltage gated channel, Shab-related subfamily, member 1
Synonyms: Kv2.1, Kcr1-1, Shab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16500
HGNC: HGNC:6231
Homologene: 37988
Ccdc28a
Name: coiled-coil domain containing 28A
Synonyms: 1700009P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215814
Homologene: 56705
Ptprr
Name: protein tyrosine phosphatase, receptor type, R
Synonyms: PTPBR7, PTP-SL, RPTPRR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19279
HGNC: HGNC:9680
Homologene: 2135
Or5d14
Name: olfactory receptor family 5 subfamily D member 14
Synonyms: GA_x6K02T2Q125-49542541-49541597, MOR174-14, Olfr1162
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258105
Homologene: 79476
Ufsp2
Name: UFM1-specific peptidase 2
Synonyms: 1810047C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 192169
Homologene: 10151
Hrh1
Name: histamine receptor H1
Synonyms: Bphs, Hir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15465
HGNC: HGNC:5182
Homologene: 668
Or2h1b
Name: olfactory receptor family 2 subfamily H member 1B
Synonyms: MOR256-39P, GA_x6K02T2PSCP-1592036-1591098, Olfr93
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258051
HGNC: HGNC:8252
Homologene: 79436
Muc13
Name: mucin 13, epithelial transmembrane
Synonyms: 114/A10, Ly64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17063
HGNC: HGNC:7511
Homologene: 7822
Smim29
Name: small integral membrane protein 29
Synonyms: AI413582
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106672
HGNC: HGNC:1340
Homologene: 66428
Adgrg2
Name: adhesion G protein-coupled receptor G2
Synonyms: Me6, B830041D06Rik, Gpr64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 237175
HGNC: HGNC:4516
Homologene: 4208
Vsig4
Name: V-set and immunoglobulin domain containing 4
Synonyms: Z39IG, CRIg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 278180
Homologene: 48515
Rasd2
Name: RASD family, member 2
Synonyms: TEM2, TEM-2, Rhes, 4930526B11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 75141
Homologene: 8628
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • C to A, chromosome 1 at 74,293,931 bp
  • T to C, chromosome 1 at 74,903,358 bp
  • C to T, chromosome 1 at 110,925,030 bp
  • C to T, chromosome 1 at 172,102,244 bp
  • T to C, chromosome 2 at 25,273,345 bp
  • A to C, chromosome 2 at 73,909,975 bp
  • C to T, chromosome 2 at 88,049,779 bp
  • T to A, chromosome 2 at 163,636,968 bp
  • A to G, chromosome 2 at 167,105,731 bp
  • A to G, chromosome 3 at 159,498,487 bp
  • C to A, chromosome 4 at 72,138,660 bp
  • A to C, chromosome 4 at 154,039,519 bp
  • G to A, chromosome 5 at 63,806,546 bp
  • T to A, chromosome 6 at 114,480,001 bp
  • T to C, chromosome 8 at 45,985,462 bp
  • G to A, chromosome 8 at 75,221,910 bp
  • T to G, chromosome 8 at 95,005,902 bp
  • T to A, chromosome 9 at 38,109,250 bp
  • A to G, chromosome 10 at 18,224,935 bp
  • A to G, chromosome 10 at 40,077,001 bp
  • T to A, chromosome 10 at 116,162,443 bp
  • A to C, chromosome 11 at 71,123,028 bp
  • G to A, chromosome 13 at 106,892,509 bp
  • T to A, chromosome 15 at 28,438,102 bp
  • G to A, chromosome 16 at 33,815,851 bp
  • A to T, chromosome 16 at 36,754,490 bp
  • G to A, chromosome 16 at 91,818,552 bp
  • T to C, chromosome 16 at 96,430,577 bp
  • A to T, chromosome 17 at 27,565,722 bp
  • A to G, chromosome 17 at 37,151,639 bp
  • A to T, chromosome 18 at 20,471,538 bp
  • T to C, chromosome 18 at 22,524,366 bp
  • T to C, chromosome 19 at 6,371,647 bp
  • C to A, chromosome X at 96,290,501 bp
  • T to G, chromosome X at 160,482,408 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4254 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041067-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.