Strain Name:
C57BL/6J-MtgxR4256Btlr/Mmmh
Stock Number:
041069-MU
Citation ID:
RRID:MMRRC_041069-MU
Other Names:
R4256 (G1), C57BL/6J-MtgxR4256Btlr
Major Collection:

Gene Information

Dip2c
Name: disco interacting protein 2 homolog C
Synonyms: 2900024P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 208440
Homologene: 40996
Plekhm1
Name: pleckstrin homology domain containing, family M (with RUN domain) member 1
Synonyms: D330036J23Rik, AP162, B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 353047
Homologene: 8871
Vsnl1
Name: visinin-like 1
Synonyms: VILIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 26950
VEGA: 12
Homologene: 2542
Vmp1
Name: vacuole membrane protein 1
Synonyms: 4930579A11Rik, 3110098I04Rik, Tmem49, Tango5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75909
Homologene: 23686
Rasa3
Name: RAS p21 protein activator 3
Synonyms: GAPIII activator 3, Ras GTPase-activating protein III, GAPIII, R-Ras gap, scat, hlb381
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 19414
Homologene: 7217
Lsg1
Name: large 60S subunit nuclear export GTPase 1
Synonyms: D16Bwg1547e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224092
Homologene: 5917
Atad2
Name: ATPase family, AAA domain containing 2
Synonyms: 2610509G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 70472
VEGA: 15
Homologene: 6044
Inava
Name: innate immunity activator
Synonyms: 5730559C18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67313
Homologene: 10103
Nbeal1
Name: neurobeachin like 1
Synonyms: A530050O19Rik, ALS2CR17, A530083I02Rik, 2310076G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269198
Homologene: 16453
Ssh2
Name: slingshot protein phosphatase 2
Synonyms: SSH-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237860
Homologene: 14116
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 545260
Homologene: 44428
Hfm1
Name: HFM1, ATP-dependent DNA helicase homolog
Synonyms: LOC381663, A330009G12Rik, Sec63d1, Mer3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 330149
Homologene: 87103
Cdhr2
Name: cadherin-related family member 2
Synonyms: LOC268663, Pcdh24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268663
Homologene: 134510
Wdr31
Name: WD repeat domain 31
Synonyms: 5430402I10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 71354
Homologene: 11360
Zfp329
Name: zinc finger protein 329
Synonyms: 2810439M05Rik, 4632409L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 67230
Homologene: 23459
Sacs
Name: sacsin
Synonyms: E130115J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50720
Gsdma2
Name: gasdermin A2
Synonyms: 2210411P14Rik, 2210009F20Rik, 2200001G21Rik, Gsdml2, 2210006M16Rik, Gsdm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76758
Lgals12
Name: lectin, galactose binding, soluble 12
Synonyms: galectin-related inhibitor of proliferation, GRIP1, galectin-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56072
Homologene: 10462
Gm5148
Name: predicted gene 5148
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 381438
Homologene: 134322
Cpne9
Name: copine family member IX
Synonyms: A730016F12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 211232
Homologene: 84686
Mettl14
Name: methyltransferase like 14
Synonyms: G430022H21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 210529
Homologene: 10865
Ttc7
Name: tetratricopeptide repeat domain 7
Synonyms: 1110035E02Rik, 1700007L07Rik, hea, fsn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 225049
Homologene: 12515
Vmn1r64
Name: vomeronasal 1 receptor 64
Synonyms: V1rd11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 404285
Homologene: 41799
Cfap43
Name: cilia and flagella associated protein 43
Synonyms: 4930428C11Rik, 4632415N18Rik, 4930463G05Rik, D19Ertd652e, Wdr96
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 100048534
Homologene: 36425
Vmn2r112
Name: vomeronasal 2, receptor 112
Synonyms: EG628185
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 628185
Homologene: 86604
Hspa4l
Name: heat shock protein 4 like
Synonyms: APG-1, 94kDa, Osp94
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18415
Homologene: 22610
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Pcn, Cyp3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
Olfr1272
Name: olfactory receptor 1272
Synonyms: GA_x6K02T2Q125-51636504-51635578, MOR227-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258982
HGNC: HGNC:8290
Homologene: 133649
4930578I06Rik
Name: RIKEN cDNA 4930578I06 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67750
VEGA: 14
Homologene: 49859
Gm906
Name: predicted gene 906
Synonyms: LOC380882
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 380882
VEGA: 13
Homologene: 134512
Olfr1391
Name: olfactory receptor 1391
Synonyms: GA_x6K02T2QP88-6107233-6106298, MOR256-27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258460
Homologene: 17264
Padi1
Name: peptidyl arginine deiminase, type I
Synonyms: Pdi1, Pad type 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18599
Homologene: 7881
Slc7a10
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 10
Synonyms: Asc-1, D7Bwg0847e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53896
Homologene: 56767
Celf4
Name: CUGBP, Elav-like family member 4
Synonyms: BRUNOL-4, Brul4, C130060B05Rik, A230070D14Rik, Brunol4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108013
VEGA: 18
Homologene: 23202
Olfr178
Name: olfactory receptor 178
Synonyms: GA_x54KRFPKG5P-55108059-55107100, MOR184-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 258999
Homologene: 79412
Mocs2
Name: molybdenum cofactor synthesis 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17434
HGNC: HGNC:7193
Homologene: 32193
Pcdha9
Name: protocadherin alpha 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 192161
HGNC: HGNC:8664
Homologene: 130700
Rspo2
Name: R-spondin 2
Synonyms: 2610028F08Rik, ftls
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239405
VEGA: 15
Homologene: 18235
Zfp551
Name: zinc fingr protein 551
Synonyms: 9630004E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 619331
Homologene: 64631
Fbxo3
Name: F-box protein 3
Synonyms: Fba, 1200002G09Rik, 1700026K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57443
Homologene: 8133
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 60,330,948 bp
  • G to T, chromosome 1 at 136,214,350 bp
  • A to T, chromosome 2 at 90,282,062 bp
  • A to G, chromosome 2 at 104,051,165 bp
  • T to A, chromosome 3 at 37,714,609 bp
  • A to G, chromosome 3 at 40,746,003 bp
  • T to C, chromosome 3 at 123,383,605 bp
  • A to G, chromosome 4 at 62,457,438 bp
  • A to T, chromosome 4 at 140,814,778 bp
  • T to C, chromosome 5 at 106,904,797 bp
  • A to T, chromosome 5 at 145,869,195 bp
  • C to T, chromosome 6 at 113,283,023 bp
  • T to A, chromosome 7 at 5,883,896 bp
  • G to A, chromosome 7 at 12,416,391 bp
  • A to G, chromosome 7 at 12,807,913 bp
  • G to T, chromosome 7 at 35,198,715 bp
  • A to G, chromosome 8 at 13,614,532 bp
  • A to T, chromosome 11 at 49,327,477 bp
  • A to G, chromosome 11 at 77,408,183 bp
  • T to A, chromosome 11 at 86,661,188 bp
  • T to A, chromosome 11 at 98,651,932 bp
  • C to A, chromosome 11 at 103,370,934 bp
  • A to T, chromosome 12 at 11,332,055 bp
  • C to A, chromosome 13 at 9,609,056 bp
  • A to C, chromosome 13 at 50,250,105 bp
  • G to A, chromosome 13 at 54,714,005 bp
  • T to C, chromosome 13 at 114,820,803 bp
  • A to G, chromosome 14 at 61,206,337 bp
  • C to T, chromosome 14 at 63,973,209 bp
  • C to A, chromosome 15 at 43,075,911 bp
  • A to G, chromosome 15 at 58,116,856 bp
  • T to G, chromosome 16 at 30,573,243 bp
  • T to G, chromosome 16 at 58,889,780 bp
  • A to G, chromosome 17 at 22,618,412 bp
  • A to T, chromosome 17 at 87,321,401 bp
  • T to C, chromosome 18 at 25,491,201 bp
  • A to G, chromosome 18 at 37,144,711 bp
  • T to C, chromosome 18 at 60,917,316 bp
  • T to G, chromosome 19 at 7,606,716 bp
  • G to A, chromosome 19 at 47,782,405 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
(Deceased) Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4256 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041069-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.