Strain Name:
C57BL/6J-MtgxR4258Btlr/Mmmh
Stock Number:
041071-MU
Citation ID:
RRID:MMRRC_041071-MU
Other Names:
R4258 (G1), C57BL/6J-MtgxR4258Btlr
Major Collection:

Strain Information

Gdf6
Name: growth differentiation factor 6
Synonyms: BMP13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242316
HGNC: HGNC:4221
Homologene: 40883
Pum1
Name: pumilio RNA-binding family member 1
Synonyms: Pumm
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 80912
Homologene: 22830
Dock9
Name: dedicator of cytokinesis 9
Synonyms: B230309H04Rik, D14Wsu89e, Zizimin1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105445
Homologene: 41026
Epc1
Name: enhancer of polycomb homolog 1
Synonyms: 5730566F07Rik, 2400007E14Rik, A930032N02Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13831
Homologene: 32627
Washc2
Name: WASH complex subunit 2
Synonyms: C530005J20Rik, D6Wsu116e, Fam21
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 28006
Homologene: 41686
Lrba
Name: LPS-responsive beige-like anchor
Synonyms: Lba, D3Ertd775e
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 80877
HGNC: HGNC:1742
Homologene: 36205
Schip1
Name: schwannomin interacting protein 1
Synonyms: SCHIP-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 30953
Homologene: 130673
Mtf1
Name: metal response element binding transcription factor 1
Synonyms: metalloregulatory transcription factor, MTF-1, metal response element-binding transcription factor 1, Thyls
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 17764
HGNC: HGNC:7428
Homologene: 4347
Chaf1a
Name: chromatin assembly factor 1, subunit A
Synonyms: CAF-1, p150
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 27221
VEGA: 17
HGNC: HGNC:1910
Homologene: 4003
Mki67
Name: antigen identified by monoclonal antibody Ki 67
Synonyms: Ki-67, D630048A14Rik, Ki67
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 17345
HGNC: HGNC:7107
Homologene: 1814
Myo9b
Name: myosin IXb
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 17925
HGNC: HGNC:7609
Homologene: 3058
Ank3
Name: ankyrin 3, epithelial
Synonyms: Ank-3, Ankyrin-3, AnkG, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Rasa2
Name: RAS p21 protein activator 2
Synonyms: GAP1m, 5430433H21Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 114713
HGNC: HGNC:9872
Homologene: 4745
Ift80
Name: intraflagellar transport 80
Synonyms: 4921524P20Rik, Wdr56
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 68259
Homologene: 12253
Smarcd2
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
Synonyms: Baf60b
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 83796
Homologene: 20671
Unc5b
Name: unc-5 netrin receptor B
Synonyms: 6330415E02Rik, Unc5h2, D10Bwg0792e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 107449
VEGA: 10
Homologene: 32538
Dennd1b
Name: DENN domain containing 1B
Synonyms: F730008N07Rik, 4632404N19Rik, 4930467M19Rik, 6820401H01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329260
Homologene: 11739
Kif7
Name: kinesin family member 7
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16576
Homologene: 72555
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319670
VEGA: 12
Homologene: 26807
Colec12
Name: collectin sub-family member 12
Synonyms: SRCL, CL-P1, Scara4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 140792
VEGA: 18
Homologene: 34248
Arsi
Name: arylsulfatase i
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 545260
Homologene: 44428
Scaf1
Name: SR-related CTD-associated factor 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233208
Cfap58
Name: cilia and flagella associated protein 58
Synonyms: LOC381229, Ccdc147
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 381229
VEGA: 19
Homologene: 77312
Arnt2
Name: aryl hydrocarbon receptor nuclear translocator 2
Synonyms: bHLHe1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 11864
Homologene: 7230
Vmn2r2
Name: vomeronasal 2, receptor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100125586
Homologene: 129753
Gm5475
Name: predicted gene 5475
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 432982
Scn9a
Name: sodium channel, voltage-gated, type IX, alpha
Synonyms: PN1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20274
Homologene: 2237
Tdrd5
Name: tudor domain containing 5
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214575
Homologene: 18312
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 192187
Homologene: 9035
Or4k36
Name: olfactory receptor family 4 subfamily K member 36
Synonyms: GA_x6K02T2Q125-72366920-72367837, MOR248-1, Olfr1280
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258910
Homologene: 121540
Traf3ip3
Name: TRAF3 interacting protein 3
Synonyms: 6030423D04Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 215243
Homologene: 11885
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Mup6
Name: major urinary protein 6
Synonyms: Gm12544
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 620807
Homologene: 74304
Cpne9
Name: copine family member IX
Synonyms: A730016F12Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 211232
Homologene: 84686
Gbp4
Name: guanylate binding protein 4
Synonyms: Mag-2, Mpa-2, Mpa2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17472
Homologene: 128731
Il3ra
Name: interleukin 3 receptor, alpha chain
Synonyms: IL-3 receptor alpha chain, CD123, SUT-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16188
VEGA: 14
HGNC: HGNC:6012
Homologene: 48088
Cyp2c65
Name: cytochrome P450, family 2, subfamily c, polypeptide 65
Synonyms: 2210009K14Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72303
Homologene: 133566
Lipo2
Name: lipase, member O2
Synonyms: Gm8981
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 101055671
Homologene: 103863
Zfp286
Name: zinc finger protein 286
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 192651
Homologene: 56892
Or5d37
Name: olfactory receptor family 5 subfamily D member 37
Synonyms: GA_x6K02T2Q125-49585842-49584862, MOR174-11, Olfr1164
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258634
Homologene: 74077
Fbxo8
Name: F-box protein 8
Synonyms: Fbx8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50753
Homologene: 8137
Dynlt1a
Name: dynein light chain Tctex-type 1A
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 100310872
VEGA: 17
Homologene: 4754
Pkn3
Name: protein kinase N3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 263803
Homologene: 50980
Sh3tc1
Name: SH3 domain and tetratricopeptide repeats 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231147
Homologene: 10360
Cyp3a25
Name: cytochrome P450, family 3, subfamily a, polypeptide 25
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56388
Homologene: 135775
Edil3
Name: EGF-like repeats and discoidin I-like domains 3
Synonyms: Del1, developmental endothelial locus-1, Del-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13612
HGNC: HGNC:3173
Homologene: 21166
Or10al6
Name: olfactory receptor family 10 subfamily AL member 6
Synonyms: GA_x6K02T2PSCP-2230932-2231897, MOR263-10, Olfr122
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 258285
Homologene: 122777
Or10j5
Name: olfactory receptor family 10 subfamily J member 5
Synonyms: MOR23, MOR267-13, GA_x6K02T2R7CC-893157-892228, Olfr16
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18313
Homologene: 7460
Psg22
Name: pregnancy-specific beta-1-glycoprotein 22
Synonyms: cea9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243862
HGNC: HGNC:1816
Homologene: 110989
Brdt
Name: bromodomain, testis-specific
Synonyms: 7420412D09Rik, Brd6, Fsrg3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 114642
HGNC: HGNC:1105
Homologene: 21064
Aspg
Name: asparaginase
Synonyms: A530050D06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104816
VEGA: 12
Homologene: 113390
Ppil6
Name: peptidylprolyl isomerase (cyclophilin)-like 6
Synonyms: 2900084F20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 73075
VEGA: 10
Homologene: 52155
Nudt16l2
Name: nudix hydrolase 16 like 2
Synonyms: 1700080E11Rik, nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 73532
Homologene: 87061
Zfp606
Name: zinc finger protein 606
Synonyms: 2410022M24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67370
Homologene: 23514
Tnfaip8
Name: tumor necrosis factor, alpha-induced protein 8
Synonyms: Gg2-1, Ssc-2, Nded, E130304C20Rik, Gm10539, Tipe
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 106869
Homologene: 8649
Gm6430
Name: predicted gene 6430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 623430
Pcdhgb2
Name: protocadherin gamma subfamily B, 2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93700
HGNC: HGNC:8709
Homologene: 49571
Ighv9-4
Name: immunoglobulin heavy variable 9-4
Synonyms: Gm7175
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 636260
Mark1-ps3
Name: MAP/microtubule affinity-regulating kinase 1, pseudogene 3
Synonyms: Gm19518
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Ssxb17
Name: SSX member B17
Synonyms: Gm21876
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 97,024,836 bp
  • G to T, chromosome 1 at 139,062,940 bp
  • T to A, chromosome 1 at 156,259,742 bp
  • C to T, chromosome 1 at 172,957,638 bp
  • C to T, chromosome 1 at 193,197,946 bp
  • C to T, chromosome 2 at 30,088,560 bp
  • A to T, chromosome 2 at 66,565,054 bp
  • T to A, chromosome 2 at 88,093,018 bp
  • A to T, chromosome 2 at 111,315,638 bp
  • T to G, chromosome 3 at 64,134,697 bp
  • T to A, chromosome 3 at 68,618,630 bp
  • A to G, chromosome 3 at 68,918,073 bp
  • A to G, chromosome 3 at 86,445,349 bp
  • G to A, chromosome 4 at 9,844,877 bp
  • A to T, chromosome 4 at 60,004,812 bp
  • A to G, chromosome 4 at 124,838,783 bp
  • G to A, chromosome 4 at 130,730,280 bp
  • C to T, chromosome 5 at 35,706,978 bp
  • T to A, chromosome 5 at 105,136,975 bp
  • A to G, chromosome 5 at 107,359,909 bp
  • T to C, chromosome 5 at 145,991,438 bp
  • C to T, chromosome 6 at 113,283,023 bp
  • C to A, chromosome 6 at 116,208,241 bp
  • T to A, chromosome 7 at 12,494,340 bp
  • T to A, chromosome 7 at 18,724,629 bp
  • G to A, chromosome 7 at 45,007,342 bp
  • G to T, chromosome 7 at 79,710,513 bp
  • T to C, chromosome 7 at 84,310,955 bp
  • A to T, chromosome 7 at 135,695,288 bp
  • A to T, chromosome 8 at 56,588,041 bp
  • T to C, chromosome 8 at 71,355,765 bp
  • A to T, chromosome 9 at 96,557,380 bp
  • T to C, chromosome 9 at 105,143,568 bp
  • T to A, chromosome 10 at 41,507,535 bp
  • T to C, chromosome 10 at 60,765,371 bp
  • T to C, chromosome 10 at 70,004,762 bp
  • T to C, chromosome 11 at 62,781,070 bp
  • A to T, chromosome 11 at 106,265,250 bp
  • A to G, chromosome 12 at 98,865,434 bp
  • A to T, chromosome 12 at 112,121,253 bp
  • A to T, chromosome 12 at 114,300,145 bp
  • G to T, chromosome 13 at 89,177,153 bp
  • A to T, chromosome 14 at 14,347,961 bp
  • G to A, chromosome 14 at 31,154,672 bp
  • T to C, chromosome 14 at 121,581,442 bp
  • A to G, chromosome 15 at 100,424,236 bp
  • C to T, chromosome 17 at 6,310,909 bp
  • C to A, chromosome 17 at 37,772,058 bp
  • T to A, chromosome 17 at 53,724,413 bp
  • C to A, chromosome 17 at 56,056,474 bp
  • T to A, chromosome 18 at 6,450,130 bp
  • T to C, chromosome 18 at 9,720,950 bp
  • C to T, chromosome 18 at 22,017,335 bp
  • A to T, chromosome 18 at 37,692,049 bp
  • A to G, chromosome 18 at 50,090,376 bp
  • T to C, chromosome 18 at 60,917,316 bp
  • A to T, chromosome 19 at 33,730,928 bp
  • A to C, chromosome 19 at 39,093,428 bp
  • A to T, chromosome 19 at 47,949,484 bp
  • C to T, chromosome X at 21,174,928 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4258 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041071-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.