Strain Name:
C57BL/6J-MtgxR4277Btlr/Mmmh
Stock Number:
041078-MU
Citation ID:
RRID:MMRRC_041078-MU
Other Names:
R4277 (G1), C57BL/6J-MtgxR4277Btlr
Major Collection:

Strain Information

Snx17
Name: sorting nexin 17
Synonyms: 5830447M19Rik, D5Ertd260e, b2b1625.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 266781
Homologene: 8838
Rasal2
Name: RAS protein activator like 2
Synonyms: A330066M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226525
HGNC: HGNC:9874
Homologene: 35217
Ern1
Name: endoplasmic reticulum (ER) to nucleus signalling 1
Synonyms: 9030414B18Rik, Ire1p, Ire1alpha, Ire1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78943
HGNC: HGNC:3449
Homologene: 55580
Vegfa
Name: vascular endothelial growth factor A
Synonyms: VPF, VEGF-A, VEGF120, Vegf, VEGF188, VEGF164
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22339
Homologene: 2534
Ndufs1
Name: NADH:ubiquinone oxidoreductase core subunit S1
Synonyms: 9930026A05Rik, 5830412M15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227197
HGNC: HGNC:7707
Homologene: 3670
Rbp3
Name: retinol binding protein 3, interstitial
Synonyms: Irbp, Rbp-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19661
VEGA: 14
HGNC: HGNC:9921
Homologene: 9261
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Vnn1
Name: vanin 1
Synonyms: pantetheinase, V-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 22361
Homologene: 32130
Cdh7
Name: cadherin 7, type 2
Synonyms: CDH7L1, 9330156F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241201
HGNC: HGNC:1766
Homologene: 68391
Jmjd8
Name: jumonji domain containing 8
Synonyms: 2610003J06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72106
Homologene: 12441
Nsun4
Name: NOL1/NOP2/Sun domain family, member 4
Synonyms: 2310010O12Rik, 2810405F18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72181
Homologene: 12455
Folh1
Name: folate hydrolase 1
Synonyms: mopsm, prostate-specific membrane antigen, glutamate carboxypeptidase II, GCP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53320
Homologene: 55826
Spp2
Name: secreted phosphoprotein 2
Synonyms: spp24, 0610038O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75396
Homologene: 5058
Trpv3
Name: transient receptor potential cation channel, subfamily V, member 3
Synonyms: 1110036I10Rik, VRL3, Nh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 246788
Homologene: 17040
Ntn4
Name: netrin 4
Synonyms: beta-netrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 57764
Homologene: 10934
Tmem260
Name: transmembrane protein 260
Synonyms: 6720456H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218989
Homologene: 49506
Dstyk
Name: dual serine/threonine and tyrosine protein kinase
Synonyms: A930019K20Rik, C430014H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 213452
Homologene: 19711
Rccd1
Name: RCC1 domain containing 1
Synonyms: E430018M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269955
Homologene: 16782
Fam98c
Name: family with sequence similarity 98, member C
Synonyms: B230110F21Rik, 1110006G06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73833
Homologene: 45483
Or7d10
Name: olfactory receptor family 7 subfamily D member 10
Synonyms: 18A, MOR143-1, GA_x6K02T2PVTD-13660026-13660964, Olfr77
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258336
HGNC: HGNC:8380
Homologene: 133688
Nsun5
Name: NOL1/NOP2/Sun domain family, member 5
Synonyms: Nol1r, 9830109N13Rik, Wbscr20a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100609
Homologene: 6828
Ldhc
Name: lactate dehydrogenase C
Synonyms: Ldh-3, Ldhc4, Ldh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16833
HGNC: HGNC:6544
Homologene: 22767
Gm13857
Name: predicted gene 13857
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 102635489
Gm22904
Name: predicted gene, 22904
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 63,170,097 bp
  • T to C, chromosome 1 at 88,411,151 bp
  • T to A, chromosome 1 at 110,065,688 bp
  • G to A, chromosome 1 at 132,455,413 bp
  • A to T, chromosome 1 at 157,157,126 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • C to T, chromosome 4 at 116,034,282 bp
  • A to G, chromosome 5 at 31,196,181 bp
  • T to C, chromosome 5 at 124,732,330 bp
  • A to G, chromosome 5 at 135,370,060 bp
  • A to G, chromosome 6 at 34,421,020 bp
  • T to A, chromosome 7 at 29,152,594 bp
  • G to A, chromosome 7 at 46,866,466 bp
  • T to C, chromosome 7 at 80,320,165 bp
  • A to G, chromosome 7 at 86,762,915 bp
  • A to G, chromosome 9 at 19,920,389 bp
  • A to T, chromosome 10 at 23,898,512 bp
  • T to C, chromosome 10 at 93,741,210 bp
  • A to G, chromosome 11 at 73,296,438 bp
  • T to C, chromosome 11 at 106,407,181 bp
  • T to G, chromosome 12 at 33,060,233 bp
  • G to A, chromosome 14 at 33,958,650 bp
  • C to T, chromosome 14 at 48,477,636 bp
  • A to G, chromosome 17 at 25,829,813 bp
  • A to G, chromosome 17 at 46,031,466 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4277 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041078-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.