Strain Name:
C57BL/6J-MtgxR4294Btlr/Mmmh
Stock Number:
041083-MU
Citation ID:
RRID:MMRRC_041083-MU
Other Names:
R4294 (G1), C57BL/6J-MtgxR4294Btlr
Major Collection:

Gene Information

Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72895
Homologene: 12485
Nes
Name: nestin
Synonyms: RC2, Marc2, ESTM46, Ifaprc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Dlc1
Name: deleted in liver cancer 1
Synonyms: p122-RhoGAP, STARD12, Arhgap7, A730069N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50768
HGNC: HGNC:2897
Homologene: 4442
Phf14
Name: PHD finger protein 14
Synonyms: 1110001C23Rik, 4932409F11Rik, 5730446A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 75725
Homologene: 8775
Sgsm1
Name: small G protein signaling modulator 1
Synonyms: 2410098H20Rik, D5Bwg1524e, Rutbc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 52850
Homologene: 64485
Mapkapk3
Name: mitogen-activated protein kinase-activated protein kinase 3
Synonyms: MK3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102626
HGNC: HGNC:6888
Homologene: 55836
Gpr151
Name: G protein-coupled receptor 151
Synonyms: C130082O03Rik, GalRL, PGR7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240239
VEGA: 18
Homologene: 18754
Sec16a
Name: SEC16 homolog A, endoplasmic reticulum export factor
Synonyms: C230052J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227648
Homologene: 10533
Kif18a
Name: kinesin family member 18A
Synonyms: N-8 kinesin, B130001M12Rik, gcd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228421
Homologene: 41820
1110059E24Rik
Name: RIKEN cDNA 1110059E24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66206
Homologene: 11933
Gucy1a1
Name: guanylate cyclase 1, soluble, alpha 1
Synonyms: alpha 1 sGC, 1200016O07Rik, sGC-alpha1, Gucy1a3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 60596
HGNC: HGNC:4685
Homologene: 37360
Ticam1
Name: toll-like receptor adaptor molecule 1
Synonyms: Trif, TICAM-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106759
Homologene: 8605
Magel2
Name: MAGE family member L2
Synonyms: NDNL1, Mage-l2, nM15, ns7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 27385
HGNC: HGNC:6814
Homologene: 8460
Srsf6
Name: serine and arginine-rich splicing factor 6
Synonyms: 1210001E11Rik, Sfrs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67996
Homologene: 110783
Lbr
Name: lamin B receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98386
HGNC: HGNC:6518
Homologene: 2455
Trpv1
Name: transient receptor potential cation channel, subfamily V, member 1
Synonyms: capsaicin receptor, VR-1, OTRPC1, Vr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 193034
Homologene: 12920
Dlg3
Name: discs large MAGUK scaffold protein 3
Synonyms: SAP102, DLG3, Dlgh3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 53310
HGNC: HGNC:2902
Homologene: 41157
Nphp3
Name: nephronophthisis 3 (adolescent)
Synonyms: D330020E01Rik, pcy, nephrocystin 3, 3632410F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74025
HGNC: HGNC:7907
Homologene: 32697
Kcnv1
Name: potassium channel, subfamily V, member 1
Synonyms: 2700023A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67498
VEGA: 15
Homologene: 22811
Fhdc1
Name: FH2 domain containing 1
Synonyms: 6330505N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229474
Homologene: 18920
Abca3
Name: ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms: ABC-C, Abc3, 1810036E22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 27410
HGNC: HGNC:33
Homologene: 37437
Ush2a
Name: usherin
Synonyms: MUSH2A, A930011D15Rik, LOC269160, LOC381317, A930037M10Rik, Ushrn, Ush2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: PBP, Moca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Slc22a21
Name: solute carrier family 22 (organic cation transporter), member 21
Synonyms: Octn3, Slc22a9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56517
Homologene: 137336
Otud7a
Name: OTU domain containing 7A
Synonyms: Cezanne 2 protein, Otud7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170711
Homologene: 15642
Pcdhb5
Name: protocadherin beta 5
Synonyms: Pcdhb4A, PcdhbE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93876
HGNC: HGNC:8689
Homologene: 75103
Vmn2r74
Name: vomeronasal 2, receptor 74
Synonyms: EG546980
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 546980
Homologene: 115466
Cckar
Name: cholecystokinin A receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12425
HGNC: HGNC:1570
Homologene: 37337
Spata13
Name: spermatogenesis associated 13
Synonyms: ESTM11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219140
Homologene: 19482
Olfr1396
Name: olfactory receptor 1396
Synonyms: GA_x6K02T2QP88-6321048-6321995, MOR276-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258334
Homologene: 28537
Cyp2c55
Name: cytochrome P450, family 2, subfamily c, polypeptide 55
Synonyms: 2010318C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 72082
HGNC: HGNC:2620
Homologene: 133567
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: CLIP-115, WSCR4, Cyln2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Cyp3a11
Name: cytochrome P450, family 3, subfamily a, polypeptide 11
Synonyms: IIIAm1, Cyp3a, Pcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 13112
HGNC: HGNC:2638
Homologene: 133568
Bivm
Name: basic, immunoglobulin-like variable motif containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 246229
Homologene: 9783
Thrb
Name: thyroid hormone receptor beta
Synonyms: T3Rbeta, T3R[b], Nr1a2, TR beta, c-erbAbeta, Thrb2, Thrb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21834
Homologene: 36025
Usp36
Name: ubiquitin specific peptidase 36
Synonyms: 2700002L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72344
Homologene: 11828
Rad18
Name: RAD18 E3 ubiquitin protein ligase
Synonyms: 2810024C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 58186
Homologene: 48572
Vmn1r192
Name: vomeronasal 1 receptor 192
Synonyms: V1ri1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 252907
Homologene: 110880
Gimap8
Name: GTPase, IMAP family member 8
Synonyms: LOC243374, IAN9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243374
Homologene: 69703
Bsnd
Name: barttin CLCNK type accessory beta subunit
Synonyms: Bartter syndrome, infantile, with sensorineural deafness (Barttin)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 140475
Homologene: 14291
Nat8f1
Name: N-acetyltransferase 8 (GCN5-related) family member 1
Synonyms: 1110002I11Rik, Cml1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 66116
Homologene: 41446
Rpl27-ps3
Name: ribosomal protein L27, pseudogene 3
Synonyms: Gm6199
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 621100
VEGA: 18
G730003C15Rik
Name: RIKEN cDNA G730003C15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 44,138,633 bp
  • A to G, chromosome 1 at 59,119,836 bp
  • C to T, chromosome 1 at 181,820,702 bp
  • C to T, chromosome 1 at 188,576,251 bp
  • A to G, chromosome 2 at 26,422,155 bp
  • T to C, chromosome 2 at 109,293,053 bp
  • T to C, chromosome 2 at 162,934,716 bp
  • A to T, chromosome 3 at 82,094,759 bp
  • C to A, chromosome 3 at 84,444,826 bp
  • A to G, chromosome 3 at 87,971,934 bp
  • C to T, chromosome 4 at 106,485,158 bp
  • A to G, chromosome 5 at 53,706,497 bp
  • A to G, chromosome 5 at 113,285,404 bp
  • A to C, chromosome 5 at 134,492,313 bp
  • A to T, chromosome 5 at 145,869,195 bp
  • C to T, chromosome 6 at 11,987,097 bp
  • A to T, chromosome 6 at 48,658,957 bp
  • T to C, chromosome 6 at 85,910,655 bp
  • G to A, chromosome 6 at 112,686,769 bp
  • AT to ATT, chromosome 6 at 113,111,320 bp
  • T to G, chromosome 7 at 62,378,767 bp
  • A to G, chromosome 7 at 63,697,191 bp
  • T to A, chromosome 7 at 85,957,416 bp
  • A to G, chromosome 8 at 36,584,753 bp
  • T to A, chromosome 9 at 104,022,717 bp
  • T to A, chromosome 9 at 106,930,043 bp
  • T to C, chromosome 9 at 107,258,932 bp
  • T to A, chromosome 11 at 49,113,427 bp
  • T to C, chromosome 11 at 53,969,503 bp
  • C to T, chromosome 11 at 73,240,464 bp
  • G to A, chromosome 11 at 118,263,647 bp
  • T to A, chromosome 13 at 22,187,295 bp
  • C to T, chromosome 14 at 18,011,145 bp
  • T to A, chromosome 14 at 60,709,555 bp
  • G to A, chromosome 15 at 45,114,444 bp
  • A to G, chromosome 17 at 24,400,569 bp
  • A to G, chromosome 17 at 56,271,339 bp
  • T to A, chromosome 18 at 6,332,607 bp
  • T to A, chromosome 18 at 37,322,681 bp
  • T to C, chromosome 18 at 42,578,537 bp
  • A to T, chromosome 19 at 21,598,749 bp
  • A to T, chromosome 19 at 39,011,791 bp
  • A to T, chromosome X at 100,796,682 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4294 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041083-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.