Strain Name:
C57BL/6J-MtgxR4322Btlr/Mmmh
Stock Number:
041093-MU
Citation ID:
RRID:MMRRC_041093-MU
Other Names:
R4322 (G1), C57BL/6J-MtgxR4322Btlr
Major Collection:

Gene Information

Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Mc4r
Name: melanocortin 4 receptor
Synonyms: Fatboy, Pkcp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17202
VEGA: 18
HGNC: HGNC:6932
Homologene: 4320
Bdp1
Name: B double prime 1, subunit of RNA polymerase III transcription initiation factor IIIB
Synonyms: TFIIIB150, TFIIIB90, TAF3B1, TFC5, Tfnr, G630013P12Rik, B130055N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 544971
Homologene: 34582
Rnf125
Name: ring finger protein 125
Synonyms: 4930553F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67664
VEGA: 18
Ccdc80
Name: coiled-coil domain containing 80
Synonyms: Urb, Ssg1, 2610001E17Rik, DRO1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67896
Homologene: 12206
Csn2
Name: casein beta
Synonyms: CSN2, Csnb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12991
HGNC: HGNC:2447
Homologene: 1426
Shld2
Name: shieldin complex subunit 2
Synonyms: 3110001K24Rik, Fam35a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75698
VEGA: 14
Homologene: 56840
Hjurp
Name: Holliday junction recognition protein
Synonyms: C330011F01Rik, A730008H23Rik, 6430706D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381280
Homologene: 10184
Pradc1
Name: protease-associated domain containing 1
Synonyms: 1700040I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73327
Homologene: 13008
Pot1a
Name: protection of telomeres 1A
Synonyms: 1500031H18Rik, Pot1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101185
Homologene: 32263
Exosc9
Name: exosome component 9
Synonyms: PM/Scl-75, RRP45, p6, p5, Pmscl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 50911
HGNC: HGNC:9137
Homologene: 3693
Fyb
Name: FYN binding protein
Synonyms: FYB-120/130, ADAP, B630013F22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 23880
HGNC: HGNC:4036
Homologene: 22664
Alx3
Name: aristaless-like homeobox 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 11694
HGNC: HGNC:449
Homologene: 4737
Mybpc3
Name: myosin binding protein C, cardiac
Synonyms: cardiac C-protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17868
HGNC: HGNC:7551
Homologene: 215
Cdhr2
Name: cadherin-related family member 2
Synonyms: LOC268663, Pcdh24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268663
Homologene: 134510
Srgap1
Name: SLIT-ROBO Rho GTPase activating protein 1
Synonyms: 4930572H05Rik, Arhgap13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 117600
Homologene: 56898
Arhgef10l
Name: Rho guanine nucleotide exchange factor (GEF) 10-like
Synonyms: 2810441C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72754
Homologene: 10017
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Ssc5d
Name: scavenger receptor cysteine rich family, 5 domains
Synonyms: s5d-srcrb, A430110N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269855
Homologene: 77555
Olfr494
Name: olfactory receptor 494
Synonyms: GA_x6K02T2PBJ9-10697517-10698461, MOR204-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258732
Homologene: 133602
Hipk3
Name: homeodomain interacting protein kinase 3
Synonyms: DYRK6, FIST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15259
HGNC: HGNC:4915
Homologene: 55923
Sh3rf2
Name: SH3 domain containing ring finger 2
Synonyms: RNF158, 9130023G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269016
Homologene: 17618
Lilrb4a
Name: leukocyte immunoglobulin-like receptor, subfamily B, member 4A
Synonyms: CD85K, ILT3, HM18, Gp49b, Lilrb4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 14728
VEGA: 10
HGNC: HGNC:6608
Homologene: 86756
Uqcrc1
Name: ubiquinol-cytochrome c reductase core protein 1
Synonyms: 1110032G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22273
Homologene: 2525
Pira13
Name: paired-Ig-like receptor A13
Synonyms: ENSMUSG00000074419, Gm15448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100041146
HGNC: HGNC:6607
Homologene: 134028
Olfr1392
Name: olfactory receptor 1392
Synonyms: GA_x6K02T2QP88-6141322-6140387, MOR256-25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258462
Homologene: 81347
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Ggcx
Name: gamma-glutamyl carboxylase
Synonyms: vitamin K-dependent carboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56316
HGNC: HGNC:4247
Homologene: 639
Kng1
Name: kininogen 1
Synonyms: L-kininogen, H-kininigen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16644
HGNC: HGNC:6383
Homologene: 88343
Actrt2
Name: actin-related protein T2
Synonyms: Arp-T2, 1700052K15Rik, Arpm2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73353
Homologene: 23647
Vmn1r203
Name: vomeronasal 1 receptor 203
Synonyms: V1rh11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 171270
Homologene: 110880
Olfr1257
Name: olfactory receptor 1257
Synonyms: GA_x6K02T2Q125-51319458-51320387, MOR232-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258984
Homologene: 115501
Atxn7l2
Name: ataxin 7-like 2
Synonyms: 2610528J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72522
Homologene: 17728
Asb13
Name: ankyrin repeat and SOCS box-containing 13
Synonyms: 6430573K02Rik, 2210015B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 142688
Homologene: 11666
Them5
Name: thioesterase superfamily member 5
Synonyms: 1110007B02Rik, 1110038F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66198
Homologene: 11931
Vtn
Name: vitronectin
Synonyms: Vn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 22370
Homologene: 532
Lmcd1
Name: LIM and cysteine-rich domains 1
Synonyms: dyxin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 30937
HGNC: HGNC:6633
Homologene: 22823
Allc
Name: allantoicase
Synonyms: 1700012B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 94041
Homologene: 6202
Dnajb14
Name: DnaJ heat shock protein family (Hsp40) member B14
Synonyms: 5730496F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 70604
Homologene: 57015
Gm12250
Name: predicted gene 12250
Synonyms: Irgb10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 631323
Homologene: 115390
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • G to C, chromosome 1 at 88,277,215 bp
  • C to A, chromosome 2 at 69,425,991 bp
  • A to G, chromosome 2 at 89,881,734 bp
  • A to G, chromosome 2 at 91,123,961 bp
  • C to A, chromosome 2 at 104,446,571 bp
  • T to C, chromosome 3 at 36,555,547 bp
  • C to A, chromosome 3 at 94,346,156 bp
  • C to T, chromosome 3 at 107,595,375 bp
  • A to T, chromosome 3 at 108,205,832 bp
  • A to G, chromosome 3 at 127,681,435 bp
  • G to A, chromosome 3 at 137,885,299 bp
  • G to A, chromosome 4 at 108,588,776 bp
  • C to A, chromosome 4 at 140,542,726 bp
  • G to A, chromosome 4 at 154,667,244 bp
  • C to T, chromosome 5 at 87,698,027 bp
  • T to C, chromosome 6 at 25,745,930 bp
  • A to G, chromosome 6 at 72,428,820 bp
  • A to G, chromosome 6 at 85,447,643 bp
  • A to G, chromosome 6 at 112,315,763 bp
  • A to G, chromosome 7 at 3,822,755 bp
  • C to T, chromosome 7 at 4,928,450 bp
  • C to T, chromosome 7 at 108,368,348 bp
  • T to C, chromosome 9 at 108,944,724 bp
  • T to C, chromosome 10 at 51,491,611 bp
  • T to C, chromosome 10 at 121,869,806 bp
  • G to A, chromosome 11 at 49,293,676 bp
  • G to A, chromosome 11 at 58,188,300 bp
  • A to T, chromosome 11 at 78,500,090 bp
  • C to A, chromosome 12 at 28,554,024 bp
  • G to T, chromosome 13 at 3,645,012 bp
  • T to A, chromosome 13 at 18,823,790 bp
  • A to T, chromosome 13 at 22,524,238 bp
  • A to T, chromosome 13 at 54,733,721 bp
  • T to A, chromosome 13 at 100,092,223 bp
  • G to T, chromosome 14 at 34,259,675 bp
  • T to A, chromosome 15 at 6,580,819 bp
  • A to C, chromosome 16 at 23,079,520 bp
  • G to T, chromosome 16 at 45,095,588 bp
  • G to A, chromosome 18 at 20,977,760 bp
  • A to T, chromosome 18 at 42,111,399 bp
  • T to C, chromosome 18 at 66,859,050 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4322 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041093-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.