Strain Name:
C57BL/6J-MtgxR4324Btlr/Mmmh
Stock Number:
041095-MU
Citation ID:
RRID:MMRRC_041095-MU
Other Names:
R4324 (G1), C57BL/6J-MtgxR4324Btlr
Major Collection:

Gene Information

Enc1
Name: ectodermal-neural cortex 1
Synonyms: Nrpb, PIG10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13803
VEGA: 13
HGNC: HGNC:3345
Homologene: 2694
Arhgef10
Name: Rho guanine nucleotide exchange factor (GEF) 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234094
Homologene: 22827
Pcbp3
Name: poly(rC) binding protein 3
Synonyms: AlphaCP-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 59093
HGNC: HGNC:8651
Homologene: 23233
Cdk5rap2
Name: CDK5 regulatory subunit associated protein 2
Synonyms: an, 2900018K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214444
Homologene: 49533
Ccdc191
Name: coiled-coil domain containing 191
Synonyms: 2610015P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212153
Homologene: 19484
Rnf125
Name: ring finger protein 125
Synonyms: 4930553F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67664
VEGA: 18
Gad1
Name: glutamate decarboxylase 1
Synonyms: GAD25, GAD67, EP10, GAD44, Gad-1, Z49976
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14415
HGNC: HGNC:4092
Homologene: 635
Man2a1
Name: mannosidase 2, alpha 1
Synonyms: Mana-2, Map-2, Mana2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 17158
HGNC: HGNC:6824
Homologene: 1777
Uqcrfs1
Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
Synonyms: 4430402G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66694
Homologene: 4378
Plekhm2
Name: pleckstrin homology domain containing, family M (with RUN domain) member 2
Synonyms: 2310034J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69582
Homologene: 19575
Trpc7
Name: transient receptor potential cation channel, subfamily C, member 7
Synonyms: Trrp8, TRP7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26946
Homologene: 22689
Exosc9
Name: exosome component 9
Synonyms: PM/Scl-75, p5, RRP45, Pmscl1, p6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 50911
HGNC: HGNC:9137
Homologene: 3693
H2-K1
Name: histocompatibility 2, K1, K region
Synonyms: H-2K
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14972
HGNC: HGNC:4931
Homologene: 128352
Ttn
Name: titin
Synonyms: D830007G01Rik, connectin, 1100001C23Rik, 2310036G12Rik, mdm, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru, L56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Dok7
Name: docking protein 7
Synonyms: EF-12, Oit5, A930013K19Rik, Dok-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231134
Homologene: 18210
Dnah17
Name: dynein, axonemal, heavy chain 17
Synonyms: Dnahcl1, Dnahc17, LOC382552, 2810003K23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69926
HGNC: HGNC:2946
Homologene: 72102
Afp
Name: alpha fetoprotein
Synonyms: alpha-foetoprotein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11576
HGNC: HGNC:317
Homologene: 36278
Stac3
Name: SH3 and cysteine rich domain 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237611
Homologene: 17039
Ccdc150
Name: coiled-coil domain containing 150
Synonyms: 4930511H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 78016
Homologene: 15814
Wipi1
Name: WD repeat domain, phosphoinositide interacting 1
Synonyms: D11Ertd498e, 4930533H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52639
Homologene: 117983
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Hipk3
Name: homeodomain interacting protein kinase 3
Synonyms: FIST3, DYRK6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15259
HGNC: HGNC:4915
Homologene: 55923
Fbxw24
Name: F-box and WD-40 domain protein 24
Synonyms: Gm5162
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382106
Homologene: 110776
Shc4
Name: SHC (Src homology 2 domain containing) family, member 4
Synonyms: 6230417E10Rik, LOC271849, 9930029B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 271849
Homologene: 19567
Vmn2r52
Name: vomeronasal 2, receptor 52
Synonyms: EG384534
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384534
Homologene: 129605
Dsel
Name: dermatan sulfate epimerase-like
Synonyms: 9330132E09Rik, DS-epi2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 319901
Homologene: 12964
Olfr473
Name: olfactory receptor 473
Synonyms: GA_x6K02T2PBJ9-10262759-10263691, MOR204-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258771
Homologene: 74116
Krt82
Name: keratin 82
Synonyms: Krt2-20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 114566
VEGA: 15
HGNC: HGNC:6459
Homologene: 13200
Cdkn2aip
Name: CDKN2A interacting protein
Synonyms: 4921511I16Rik, CARF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 70925
Homologene: 9752
Spag6l
Name: sperm associated antigen 6-like
Synonyms: PF16, Spag6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 50525
Homologene: 8252
Prss3
Name: protease, serine 3
Synonyms: TRY4, Try3, Tb, mesotrypsin, MTG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22073
Homologene: 88408
Atg10
Name: autophagy related 10
Synonyms: 5430428K15Rik, 5330424L23Rik, APG10, Apg10p, Apg10l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66795
VEGA: 13
Homologene: 12036
Dzank1
Name: double zinc ribbon and ankyrin repeat domains 1
Synonyms: 2810039F03Rik, 6330439K17Rik, Ankrd64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241688
Homologene: 10037
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Cdk14
Name: cyclin-dependent kinase 14
Synonyms: Pftk1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18647
HGNC: HGNC:8883
Homologene: 7888
Cntn6
Name: contactin 6
Synonyms: NB-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 53870
HGNC: HGNC:2176
Homologene: 8702
Ggcx
Name: gamma-glutamyl carboxylase
Synonyms: vitamin K-dependent carboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56316
HGNC: HGNC:4247
Homologene: 639
Pcgf1
Name: polycomb group ring finger 1
Synonyms: 2010002K04Rik, Nspc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69837
Homologene: 13090
Atxn7l2
Name: ataxin 7-like 2
Synonyms: 2610528J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72522
Homologene: 17728
Asb13
Name: ankyrin repeat and SOCS box-containing 13
Synonyms: 2210015B19Rik, 6430573K02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 142688
Homologene: 11666
Ppp1r42
Name: protein phosphatase 1, regulatory subunit 42
Synonyms: 1700011J18Rik, Lrrc67, 4930418G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69312
Homologene: 27077
Epn1
Name: epsin 1
Synonyms: Ibp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 13854
Homologene: 32172
Cers4
Name: ceramide synthase 4
Synonyms: CerS4, Lass4, 2900019C14Rik, Trh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67260
Homologene: 11582
Alg9
Name: asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase)
Synonyms: 8230402H15Rik, B430313H07Rik, Dibd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102580
Homologene: 6756
Aqp6
Name: aquaporin 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11831
VEGA: 15
HGNC: HGNC:639
Homologene: 20399
Fam115a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 9,999,494 bp
  • A to G, chromosome 1 at 54,361,028 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to C, chromosome 1 at 111,861,393 bp
  • G to A, chromosome 2 at 70,589,830 bp
  • T to C, chromosome 2 at 76,908,579 bp
  • C to A, chromosome 2 at 104,446,571 bp
  • T to A, chromosome 2 at 125,678,750 bp
  • T to C, chromosome 2 at 144,488,698 bp
  • T to C, chromosome 3 at 36,552,670 bp
  • T to C, chromosome 3 at 36,555,547 bp
  • A to T, chromosome 3 at 108,205,832 bp
  • A to G, chromosome 3 at 127,681,435 bp
  • A to T, chromosome 4 at 70,353,614 bp
  • A to T, chromosome 4 at 141,631,857 bp
  • T to A, chromosome 5 at 5,036,532 bp
  • A to T, chromosome 5 at 35,079,797 bp
  • A to G, chromosome 5 at 90,507,905 bp
  • T to G, chromosome 6 at 41,373,845 bp
  • A to G, chromosome 6 at 42,676,744 bp
  • A to G, chromosome 6 at 72,428,820 bp
  • T to C, chromosome 6 at 83,079,957 bp
  • G to A, chromosome 6 at 104,569,143 bp
  • T to A, chromosome 7 at 5,097,211 bp
  • T to A, chromosome 7 at 10,171,013 bp
  • C to T, chromosome 7 at 107,933,693 bp
  • T to C, chromosome 8 at 4,523,621 bp
  • T to A, chromosome 8 at 14,940,335 bp
  • A to T, chromosome 8 at 47,712,173 bp
  • T to G, chromosome 9 at 50,805,343 bp
  • C to T, chromosome 9 at 109,604,945 bp
  • G to A, chromosome 10 at 76,763,343 bp
  • C to T, chromosome 10 at 127,503,249 bp
  • A to T, chromosome 11 at 109,603,836 bp
  • C to T, chromosome 11 at 118,094,213 bp
  • G to T, chromosome 13 at 3,645,012 bp
  • A to G, chromosome 13 at 30,541,158 bp
  • T to C, chromosome 13 at 56,887,356 bp
  • T to A, chromosome 13 at 91,040,966 bp
  • T to C, chromosome 13 at 97,245,897 bp
  • G to A, chromosome 15 at 99,601,410 bp
  • T to A, chromosome 15 at 101,541,747 bp
  • T to C, chromosome 16 at 16,787,235 bp
  • A to G, chromosome 16 at 43,947,509 bp
  • C to T, chromosome 17 at 34,000,040 bp
  • A to G, chromosome 17 at 64,666,793 bp
  • G to A, chromosome 18 at 20,977,760 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4324 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041095-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.