Strain Name:
C57BL/6J-MtgxR4326Btlr/Mmmh
Stock Number:
041096-MU
Citation ID:
RRID:MMRRC_041096-MU
Other Names:
R4326 (G1), C57BL/6J-MtgxR4326Btlr
Major Collection:

Gene Information

Ppp2r2d
Name: protein phosphatase 2, regulatory subunit B, delta
Synonyms: D7Ertd753e, MDS026, 1300017E19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52432
Homologene: 81915
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, trabeculin alpha, Aclp7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11426
Homologene: 136191
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56381
Homologene: 124461
Zfp934
Name: zinc finger protein 934
Synonyms: 6720457D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 77117
VEGA: 13
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Eh domain, SH3 domain regulator of endocytosis 1, EHSH1, Sh3p17, Ese1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Pafah1b1
Name: platelet-activating factor acetylhydrolase, isoform 1b, subunit 1
Synonyms: lissencephaly-1 protein, PAF-AH 45, Lis1, Mdsh, Pafaha, LIS-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18472
HGNC: HGNC:8574
Homologene: 371
Arap2
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Synonyms: Centd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212285
Homologene: 9064
Sin3a
Name: transcriptional regulator, SIN3A (yeast)
Synonyms: mSin3A, Sin3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 20466
Homologene: 32124
Ipo8
Name: importin 8
Synonyms: OM-1, Om1, C130009K11Rik, 6230418K12Rik, Ranbp8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320727
HGNC: HGNC:9853
Homologene: 48430
Prdm15
Name: PR domain containing 15
Synonyms: Zfp298, E130018M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 114604
Homologene: 56941
St7
Name: suppression of tumorigenicity 7
Synonyms: Fam4a2, HELG, 9430001H04Rik, RAY1, TSG7, SEN4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64213
Homologene: 10185
Cd55
Name: CD55 molecule, decay accelerating factor for complement
Synonyms: Daf-GPI, Cromer blood group, Daf1, GPI-DAF, complement-glycosylphosphatidylinositol
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13136
HGNC: HGNC:2665
Homologene: 479
Lmo7
Name: LIM domain only 7
Synonyms: FBXO20, LOC380928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 380928
HGNC: HGNC:6646
Homologene: 83924
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Dnahc8, P1-Loop, Hst6.7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Tcf25
Name: transcription factor 25 (basic helix-loop-helix)
Synonyms: D8Ertd325e, 1100001J13Rik, Nulp1, 1810041K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66855
Homologene: 5701
Zfta
Name: zinc finger translocation associated
Synonyms: 2700081O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 108899
Homologene: 52179
Tmem107
Name: transmembrane protein 107
Synonyms: 1110004B13Rik, 2810049P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66910
Homologene: 12052
Isx
Name: intestine specific homeobox
Synonyms: 9130012O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 71597
Homologene: 28312
Fastkd2
Name: FAST kinase domains 2
Synonyms: 2810421I24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75619
Homologene: 8957
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, Nesp2g, Cpfl8, diminished cone electroretinogram, dice, 6820443O06Rik, D12Ertd777e, syne-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319565
Homologene: 56700
Ttc8
Name: tetratricopeptide repeat domain 8
Synonyms: 0610012F22Rik, BBS8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76260
Homologene: 14988
Tmem62
Name: transmembrane protein 62
Synonyms: B830009D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 96957
Homologene: 11801
Lingo2
Name: leucine rich repeat and Ig domain containing 2
Synonyms: B230217C06Rik, LERN3, Lrrn6c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242384
Homologene: 17621
Wdr90
Name: WD repeat domain 90
Synonyms: 3230401M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106618
Homologene: 27066
Capn13
Name: calpain 13
Synonyms: LOC381122
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 381122
VEGA: 17
Homologene: 27026
Adamts15
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235130
VEGA: 9
Homologene: 8610
Lpcat4
Name: lysophosphatidylcholine acyltransferase 4
Synonyms: Agpat7, Aytl3, LPEAT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99010
Homologene: 27544
Pcdhb9
Name: protocadherin beta 9
Synonyms: PcdhbI, Pcdhb4C
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 93880
Homologene: 87124
Arrdc5
Name: arrestin domain containing 5
Synonyms: 1700013E09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 76920
VEGA: 17
Homologene: 66277
Noxo1
Name: NADPH oxidase organizer 1
Synonyms: 2310034C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 71893
Homologene: 12418
Slc13a1
Name: solute carrier family 13 (sodium/sulfate symporters), member 1
Synonyms: Nas1, NaSi-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 55961
Homologene: 31893
Ssrp1
Name: structure specific recognition protein 1
Synonyms: T160, Hmgi-rs3, Hmgox, Hmg1-rs1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20833
Homologene: 110735
Pak1ip1
Name: PAK1 interacting protein 1
Synonyms: 5830431I15Rik, Gdpd1, PIP1, 5930415H02Rik, p21-activated protein kinase-interacting protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68083
Homologene: 39574
Ro60
Name: Ro60, Y RNA binding protein
Synonyms: Ssa2, Ssa, Trove2, A530054J02Rik, 1810007I17Rik, SS-A/Ro
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20822
Homologene: 3383
Zfp184
Name: zinc finger protein 184 (Kruppel-like)
Synonyms: 4930500C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 193452
Homologene: 113602
Krt80
Name: keratin 80
Synonyms: 1200016G03Rik, 2310041I20Rik, Kb20
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 74127
VEGA: 15
Homologene: 66610
Olfr479
Name: olfactory receptor 479
Synonyms: MOR267-15, GA_x6K02T2PBJ9-10384085-10385068
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257891
Homologene: 79346
Klhl40
Name: kelch-like 40
Synonyms: 2310024D23Rik, Kbtbd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 72330
VEGA: 9
Homologene: 17571
Olfr854
Name: olfactory receptor 854
Synonyms: MOR147-3, GA_x6K02T2PVTD-13313295-13312357
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258515
HGNC: HGNC:8465
Homologene: 128144
Cyp2j8
Name: cytochrome P450, family 2, subfamily j, polypeptide 8
Synonyms: Cyp2j8-ps, OTTMUSG00000007938
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 665095
Homologene: 133819
Glt28d2
Name: glycosyltransferase 28 domain containing 2
Synonyms: 4732486J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 320302
Homologene: 44843
Erlec1
Name: endoplasmic reticulum lectin 1
Synonyms: 4933407N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66753
Homologene: 9247
Abhd17a
Name: abhydrolase domain containing 17A
Synonyms: Fam108a, 1700013O15Rik, D10Bwg1364e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216169
Homologene: 50272
Ccdc113
Name: coiled-coil domain containing 113
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244608
Homologene: 40927
Gm12367
Name: predicted gene 12367
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
5033430I15Rik
Name: RIKEN cDNA 5033430I15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76000
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 63,752,357 bp
  • A to T, chromosome 1 at 130,452,483 bp
  • G to A, chromosome 1 at 143,766,707 bp
  • A to G, chromosome 2 at 85,040,217 bp
  • G to A, chromosome 2 at 112,246,392 bp
  • T to A, chromosome 2 at 120,980,510 bp
  • G to A, chromosome 3 at 85,872,086 bp
  • T to A, chromosome 4 at 35,225,985 bp
  • T to A, chromosome 4 at 35,708,462 bp
  • T to A, chromosome 4 at 96,507,329 bp
  • C to T, chromosome 4 at 123,382,212 bp
  • T to G, chromosome 4 at 141,477,372 bp
  • A to T, chromosome 5 at 62,621,863 bp
  • G to A, chromosome 6 at 17,819,288 bp
  • A to G, chromosome 6 at 24,103,479 bp
  • A to T, chromosome 6 at 148,800,164 bp
  • T to C, chromosome 7 at 108,055,155 bp
  • T to A, chromosome 7 at 138,868,485 bp
  • A to G, chromosome 8 at 74,873,656 bp
  • G to A, chromosome 8 at 95,557,268 bp
  • T to A, chromosome 8 at 123,401,143 bp
  • T to A, chromosome 9 at 19,567,022 bp
  • T to C, chromosome 9 at 30,904,518 bp
  • T to C, chromosome 9 at 57,095,358 bp
  • A to G, chromosome 9 at 121,778,890 bp
  • A to G, chromosome 10 at 80,584,050 bp
  • C to T, chromosome 11 at 30,949,972 bp
  • G to T, chromosome 11 at 69,071,475 bp
  • T to C, chromosome 11 at 74,682,240 bp
  • C to A, chromosome 12 at 75,952,742 bp
  • T to C, chromosome 12 at 98,946,586 bp
  • T to C, chromosome 13 at 21,959,902 bp
  • A to G, chromosome 13 at 41,004,756 bp
  • T to C, chromosome 13 at 45,965,967 bp
  • T to G, chromosome 13 at 62,517,559 bp
  • A to T, chromosome 14 at 101,900,074 bp
  • C to T, chromosome 15 at 101,352,308 bp
  • A to G, chromosome 16 at 91,853,855 bp
  • G to T, chromosome 16 at 97,806,515 bp
  • G to A, chromosome 17 at 24,698,963 bp
  • G to T, chromosome 17 at 25,853,731 bp
  • G to A, chromosome 17 at 30,752,092 bp
  • T to C, chromosome 17 at 56,294,420 bp
  • C to A, chromosome 17 at 73,331,108 bp
  • A to T, chromosome 18 at 37,401,822 bp
  • G to T, chromosome 18 at 37,401,823 bp
  • A to G, chromosome 19 at 7,421,226 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4326 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041096-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.