Strain Name:
C57BL/6J-MtgxR4349Btlr/Mmmh
Stock Number:
041104-MU
Citation ID:
RRID:MMRRC_041104-MU
Other Names:
R4349 (G1), C57BL/6J-MtgxR4349Btlr
Major Collection:

Strain Information

Nrp2
Name: neuropilin 2
Synonyms: NP2, Npn-2, Npn2, NP-2, 1110048P06Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 18187
HGNC: HGNC:8005
Homologene: 2875
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, separase, SSE, ESP1, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Ptch1
Name: patched 1
Synonyms: Ptc, Patched 1, Ptc1, A230106A15Rik, wig
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 19206
HGNC: HGNC:9585
Homologene: 223
Bahcc1
Name: BAH domain and coiled-coil containing 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 268515
Homologene: 129585
Pigv
Name: phosphatidylinositol glycan anchor biosynthesis, class V
Synonyms: D430024F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230801
Homologene: 5354
Rbm25
Name: RNA binding motif protein 25
Synonyms: 2610015J01Rik, A130095G20Rik, 2600011C06Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 67039
Sec24a
Name: SEC24 homolog A, COPII coat complex component
Synonyms: 9430090N21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 77371
Homologene: 70131
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Kif23
Name: kinesin family member 23
Synonyms: 3110001D19Rik, CHO1, MKLP-1, MKLP1, Knsl5, C87313
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71819
VEGA: 9
HGNC: HGNC:6392
Homologene: 11491
Acbd6
Name: acyl-Coenzyme A binding domain containing 6
Synonyms: 0610010G04Rik, 2610100E10Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 72482
Homologene: 12465
Sidt2
Name: SID1 transmembrane family, member 2
Synonyms: CGI-40
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214597
Homologene: 22943
Marchf7
Name: membrane associated ring-CH-type finger 7
Synonyms: Axot, Gtrgeo17, March7
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 57438
Homologene: 10753
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Milr1
Name: mast cell immunoglobulin like receptor 1
Synonyms: LOC380732, Allergin-1, Gm885
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380732
Homologene: 86017
Tet3
Name: tet methylcytosine dioxygenase 3
Synonyms: D230004J03Rik, B430006D22Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194388
Homologene: 35360
Zfp605
Name: zinc finger protein 605
Synonyms: A830023I12Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 675812
Homologene: 52335
Otog
Name: otogelin
Synonyms: Otgn
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18419
HGNC: HGNC:8516
Homologene: 8421
Skint10
Name: selection and upkeep of intraepithelial T cells 10
Synonyms: A030001H23Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230613
Homologene: 136292
Cd101
Name: CD101 antigen
Synonyms: LOC381460, Igsf2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 630146
HGNC: HGNC:5949
Homologene: 3142
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: slo2, Slack, C030030G16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227632
Homologene: 11055
Grik1
Name: glutamate receptor, ionotropic, kainate 1
Synonyms: Glur-5, Glur5, D16Ium24, A830007B11Rik, D16Ium24e, Glurbeta1, GluK5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 14805
HGNC: HGNC:4579
Homologene: 68992
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
Vmn2r94
Name: vomeronasal 2, receptor 94
Synonyms: EG665227
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 665227
Homologene: 129751
Klhdc7a
Name: kelch domain containing 7A
Synonyms: B230308G19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242721
Homologene: 17567
Cdh20
Name: cadherin 20
Synonyms: Cdh7
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23836
HGNC: HGNC:1760
Homologene: 8015
Klhl35
Name: kelch-like 35
Synonyms: 2810406K13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 72184
Homologene: 27849
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Rnf31
Name: ring finger protein 31
Synonyms: Paul, HOIP
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268749
Homologene: 33228
Dzip1
Name: DAZ interacting protein 1
Synonyms: 2510025K24Rik, 2810422M04Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 66573
VEGA: 14
Homologene: 45708
Pacsin1
Name: protein kinase C and casein kinase substrate in neurons 1
Synonyms: A830061D09Rik, Syndapin I
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 23969
HGNC: HGNC:8570
Homologene: 22674
Or12j5
Name: olfactory receptor family 12 subfamily J member 5
Synonyms: GA_x6K02T2PBJ9-42653501-42652578, MOR252-1, MOR252-4, Olfr536
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258513
Homologene: 51731
Zfp593os
Name: Zfp593 opposite strand
Synonyms: E130218I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77490
Fra10ac1
Name: FRA10A associated CGG repeat 1
Synonyms: 5730455O13Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 70567
VEGA: 19
HGNC: HGNC:1162
Homologene: 13852
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 62,738,417 bp
  • A to G, chromosome 1 at 104,979,089 bp
  • G to T, chromosome 1 at 155,687,081 bp
  • C to T, chromosome 2 at 25,906,788 bp
  • T to C, chromosome 2 at 60,234,195 bp
  • G to A, chromosome 3 at 101,013,314 bp
  • T to C, chromosome 4 at 112,769,771 bp
  • T to C, chromosome 4 at 133,664,816 bp
  • T to C, chromosome 4 at 134,245,056 bp
  • A to G, chromosome 4 at 139,966,277 bp
  • G to A, chromosome 5 at 3,957,671 bp
  • T to C, chromosome 5 at 88,503,548 bp
  • T to A, chromosome 5 at 110,128,686 bp
  • T to C, chromosome 6 at 83,403,275 bp
  • A to T, chromosome 7 at 46,274,189 bp
  • G to A, chromosome 7 at 99,473,719 bp
  • A to T, chromosome 7 at 140,504,357 bp
  • T to C, chromosome 8 at 125,762,851 bp
  • C to T, chromosome 9 at 45,945,713 bp
  • C to T, chromosome 9 at 61,932,114 bp
  • G to T, chromosome 11 at 51,715,149 bp
  • C to T, chromosome 11 at 60,709,568 bp
  • T to C, chromosome 11 at 106,763,882 bp
  • G to A, chromosome 11 at 120,259,201 bp
  • A to G, chromosome 12 at 83,675,173 bp
  • C to T, chromosome 13 at 63,534,329 bp
  • AAC to A, chromosome 14 at 55,601,098 bp
  • T to C, chromosome 14 at 118,883,526 bp
  • C to A, chromosome 15 at 102,319,604 bp
  • T to C, chromosome 16 at 87,957,543 bp
  • T to A, chromosome 17 at 18,244,343 bp
  • G to A, chromosome 17 at 27,707,004 bp
  • A to G, chromosome 19 at 38,199,605 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4349 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041104-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.