Strain Name:
C57BL/6J-MtgxR4376Btlr/Mmmh
Stock Number:
041120-MU
Citation ID:
RRID:MMRRC_041120-MU
Other Names:
R4376 (G1), C57BL/6J-MtgxR4376Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Gsfsow3, c-KIT, SCO1, SOW3, Tr-kit, SCO5, Steel Factor Receptor, belly-spot, Gsfsco1, Gsfsco5, Dominant white spotting, CD117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Suz12
Name: SUZ12 polycomb repressive complex 2 subunit
Synonyms: D11Ertd530e, 2610028O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52615
Homologene: 32256
Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, Mll3, HALR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231051
Homologene: 46480
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: ESP1, Cerp, separase, PRCE, PRCE, SSE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Gon4l
Name: gon-4 like
Synonyms: 1500041I23Rik, 2610100B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76022
Homologene: 13002
Rictor
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 6030405M08Rik, D530039E11Rik, 4921505C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78757
VEGA: 15
Homologene: 34317
Osbpl8
Name: oxysterol binding protein-like 8
Synonyms: D330025H14Rik, ORP-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237542
VEGA: 10
Homologene: 68813
Prcc
Name: papillary renal cell carcinoma (translocation-associated)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 94315
HGNC: HGNC:9343
Homologene: 38120
Steep1
Name: STING1 ER exit protein 1
Synonyms: C330007P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 77644
Homologene: 11133
Mlh3
Name: mutL homolog 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217716
VEGA: 12
HGNC: HGNC:7128
Homologene: 91153
Pole
Name: polymerase (DNA directed), epsilon
Synonyms: pol-epsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18973
HGNC: HGNC:9177
Homologene: 4539
Pdia4
Name: protein disulfide isomerase associated 4
Synonyms: Erp72, Cai, U48620, ERp72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12304
Homologene: 21020
Ppp3cb
Name: protein phosphatase 3, catalytic subunit, beta isoform
Synonyms: PP2BA beta, CnAbeta, Cnab, Calnb, 1110063J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 19056
HGNC: HGNC:9315
Homologene: 56429
Marchf11
Name: membrane associated ring-CH-type finger 11
Synonyms: March11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 211147
Homologene: 45584
Aldob
Name: aldolase B, fructose-bisphosphate
Synonyms: Aldo2, Aldo-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230163
HGNC: HGNC:417
Homologene: 20060
Eaf2
Name: ELL associated factor 2
Synonyms: Traits, Festa, FESTA-L, FESTA-S, U19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106389
Homologene: 10208
Polq
Name: polymerase (DNA directed), theta
Synonyms: A430110D14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77782
HGNC: HGNC:9186
Homologene: 32727
Nlrp12
Name: NLR family, pyrin domain containing 12
Synonyms: Nalp12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 378425
Homologene: 16972
Ttc23l
Name: tetratricopeptide repeat domain 23-like
Synonyms: 4930401A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75777
Homologene: 44907
Plce1
Name: phospholipase C, epsilon 1
Synonyms: 4933403A21Rik, PLCepsilon
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 74055
Homologene: 9478
Or13f5
Name: olfactory receptor family 13 subfamily F member 5
Synonyms: GA_x6K02T2N78B-7168533-7167574, Olfr275, MOR262-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258857
Homologene: 17418
Mrps2
Name: mitochondrial ribosomal protein S2
Synonyms: 1500019M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 118451
Homologene: 99837
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21974
Homologene: 134711
Adamts18
Name: ADAM metallopeptidase with thrombospondin type 1 motif 18
Synonyms: E130314N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 208936
Homologene: 65241
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A3, UT-A5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
Tsnaxip1
Name: translin-associated factor X (Tsnax) interacting protein 1
Synonyms: 1700016K08Rik, TXI1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72236
Homologene: 10194
Adgrg6
Name: adhesion G protein-coupled receptor G6
Synonyms: Gpr126, DREG, LOC215798, 1190004A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215798
Homologene: 10724
Fam83e
Name: family with sequence similarity 83, member E
Synonyms: 4930403C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 73813
Homologene: 9791
Or4c122
Name: olfactory receptor family 4 subfamily C member 122
Synonyms: Olfr1228, MOR233-1, GA_x6K02T2Q125-50696209-50695274
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258973
Homologene: 74217
Pank1
Name: pantothenate kinase 1
Synonyms: 4632412I06Rik, Pank1b, Pank1, Pank1a, 5430426F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75735
VEGA: 19
HGNC: HGNC:8598
Homologene: 56979
Adck5
Name: aarF domain containing kinase 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268822
Homologene: 34378
Atp10d
Name: ATPase, class V, type 10D
Synonyms: IMAGE:1069176, D5Buc24e, 9830145H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231287
Pfn4
Name: profilin family, member 4
Synonyms: 2900024P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 382562
VEGA: 12
Homologene: 20046
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 2 at 28,468,859 bp
  • T to C, chromosome 2 at 89,249,245 bp
  • A to G, chromosome 3 at 87,867,407 bp
  • C to A, chromosome 3 at 88,907,387 bp
  • A to G, chromosome 4 at 49,538,785 bp
  • A to T, chromosome 4 at 52,826,195 bp
  • C to T, chromosome 5 at 25,315,326 bp
  • T to C, chromosome 5 at 72,296,975 bp
  • T to C, chromosome 5 at 75,640,499 bp
  • T to A, chromosome 5 at 110,337,205 bp
  • G to A, chromosome 6 at 47,798,392 bp
  • A to G, chromosome 7 at 3,240,946 bp
  • A to T, chromosome 7 at 45,723,893 bp
  • C to A, chromosome 8 at 105,841,801 bp
  • A to T, chromosome 8 at 113,773,240 bp
  • A to G, chromosome 10 at 14,438,494 bp
  • G to A, chromosome 10 at 14,469,050 bp
  • T to A, chromosome 10 at 111,269,419 bp
  • A to G, chromosome 11 at 79,999,117 bp
  • T to A, chromosome 12 at 4,770,182 bp
  • C to A, chromosome 12 at 85,259,198 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • C to A, chromosome 14 at 20,523,921 bp
  • T to C, chromosome 15 at 6,786,967 bp
  • CT to CTTGGATT, chromosome 15 at 10,537,562 bp
  • G to A, chromosome 15 at 10,537,566 bp
  • A to G, chromosome 15 at 26,309,446 bp
  • A to G, chromosome 15 at 76,594,335 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • A to G, chromosome 16 at 36,800,636 bp
  • G to T, chromosome 16 at 37,013,181 bp
  • G to A, chromosome 18 at 78,207,068 bp
  • C to T, chromosome 19 at 34,877,704 bp
  • T to C, chromosome 19 at 38,705,447 bp
  • C to A, chromosome X at 36,824,159 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4376 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041120-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.