Strain Name:
C57BL/6J-MtgxR4404Btlr/Mmmh
Stock Number:
041133-MU
Citation ID:
RRID:MMRRC_041133-MU
Other Names:
R4404 (G1), C57BL/6J-MtgxR4404Btlr
Major Collection:

Gene Information

Pank4
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 269614
Homologene: 41235
Htr6
Name: 5-hydroxytryptamine (serotonin) receptor 6
Synonyms: 5-HT6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 15565
HGNC: HGNC:5301
Homologene: 673
Npy5r
Name: neuropeptide Y receptor Y5
Synonyms: Y5R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 18168
HGNC: HGNC:7958
Homologene: 21241
Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Gluralpha4, Glur4, spkw1, Glur-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Mysm1
Name: myb-like, SWIRM and MPN domains 1
Synonyms: C530050H10Rik, C130067A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 320713
Homologene: 66204
Trpm7
Name: transient receptor potential cation channel, subfamily M, member 7
Synonyms: LTRPC7, 5033407O22Rik, CHAK1, CHAK, 2310022G15Rik, Ltpr7, TRP-PLIK, 4833414K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 58800
Homologene: 9774
Usp25
Name: ubiquitin specific peptidase 25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 30940
VEGA: 16
Homologene: 8374
Nomo1
Name: nodal modulator 1
Synonyms: D7Ertd156e, Nomo, PM5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 211548
Homologene: 13810
Dop1a
Name: DOP1 leucine zipper like protein A
Synonyms: B130005I07Rik, Dopey1, D9Ertd809e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 320615
Homologene: 26645
Fer
Name: fer (fms/fps related) protein kinase
Synonyms: Fert2, C330004K01Rik, Fert
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14158
VEGA: 17
HGNC: HGNC:3655
Homologene: 74300
Zdhhc15
Name: zinc finger, DHHC domain containing 15
Synonyms: 6030457O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 108672
Homologene: 81788
Sertm1
Name: serine rich and transmembrane domain containing 1
Synonyms: 6030405A18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 329641
Homologene: 45594
Fan1
Name: FANCD2/FANCI-associated nuclease 1
Synonyms: 6030441H18Rik, Mtmr15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 330554
Homologene: 45598
Il22ra1
Name: interleukin 22 receptor, alpha 1
Synonyms: Il22r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230828
Homologene: 10943
Nol11
Name: nucleolar protein 11
Synonyms: 1500002M01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 68979
Homologene: 32264
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 240411
Lama3
Name: laminin, alpha 3
Synonyms: nicein, 150kDa, [a]3B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 16774
HGNC: HGNC:6483
Homologene: 18279
Fcsk
Name: fucose kinase
Synonyms: Fuk, L-fucose kinase, 1110046B12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234730
Homologene: 15452
Mcc
Name: mutated in colorectal cancers
Synonyms: D18Ertd451e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 328949
HGNC: HGNC:6935
Homologene: 20539
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 210198
VEGA: 10
Homologene: 17529
Ubqlnl
Name: ubiquilin-like
Synonyms: 4922504M18Rik, LOC244179
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 244179
Homologene: 17034
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: CMF608, Adlican2, 6530405F15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242050
Homologene: 18712
Pam
Name: peptidylglycine alpha-amidating monooxygenase
Synonyms: PHM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18484
HGNC: HGNC:8596
Homologene: 37369
Pifo
Name: primary cilia formation
Synonyms: 1700027A23Rik, pitchfork
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 100503311
Homologene: 18759
Olfr935
Name: olfactory receptor 935
Synonyms: GA_x6K02T2PVTD-32691280-32690354, MOR171-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 258741
VEGA: 9
Homologene: 133683
Gtf3c4
Name: general transcription factor IIIC, polypeptide 4
Synonyms: KAT12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 269252
HGNC: HGNC:4667
Homologene: 8147
Hars2
Name: histidyl-tRNA synthetase 2
Synonyms: 4631412B19Rik, HO3, HARSR, Harsl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 70791
HGNC: HGNC:4817
Homologene: 40819
Ccdc188
Name: coiled-coil domain containing 188
Synonyms: Gm7873
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 102638083
VEGA: 16
Homologene: 90442
Slc22a2
Name: solute carrier family 22 (organic cation transporter), member 2
Synonyms: Orct2, Oct2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 20518
VEGA: 17
Homologene: 68293
S100a4
Name: S100 calcium binding protein A4
Synonyms: 42a, Capl, calvasculin, FSp1, PeL98, Mts1, pk9a, metastasin, CAPL, 18A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 20198
Homologene: 7924
Zfp353-ps
Name: zinc finger protein 353, pseudogene
Synonyms: Zfp353
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234203
C87414
Name: expressed sequence C87414
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 381654
Homologene: 133100
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 97,854,721 bp
  • T to C, chromosome 2 at 28,826,749 bp
  • C to A, chromosome 2 at 126,833,715 bp
  • C to T, chromosome 3 at 54,899,325 bp
  • T to C, chromosome 3 at 59,329,551 bp
  • T to G, chromosome 3 at 90,604,983 bp
  • G to A, chromosome 3 at 106,001,368 bp
  • T to C, chromosome 4 at 94,965,212 bp
  • A to T, chromosome 4 at 135,737,431 bp
  • A to G, chromosome 4 at 139,062,202 bp
  • C to T, chromosome 4 at 154,980,156 bp
  • T to A, chromosome 5 at 93,637,713 bp
  • A to G, chromosome 7 at 46,056,668 bp
  • A to G, chromosome 7 at 64,372,631 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • T to C, chromosome 8 at 42,082,580 bp
  • C to T, chromosome 8 at 66,681,992 bp
  • T to A, chromosome 8 at 110,890,301 bp
  • A to T, chromosome 9 at 4,464,489 bp
  • T to C, chromosome 9 at 38,994,569 bp
  • T to A, chromosome 9 at 86,522,813 bp
  • T to C, chromosome 10 at 51,628,543 bp
  • A to T, chromosome 11 at 107,173,725 bp
  • A to G, chromosome 16 at 18,218,420 bp
  • A to C, chromosome 16 at 77,115,453 bp
  • A to G, chromosome 17 at 12,614,764 bp
  • A to T, chromosome 17 at 63,941,289 bp
  • G to A, chromosome 18 at 12,582,531 bp
  • G to T, chromosome 18 at 36,785,936 bp
  • G to A, chromosome 18 at 44,759,298 bp
  • G to A, chromosome 18 at 77,431,132 bp
  • G to A, chromosome X at 104,560,688 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4404 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041133-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.