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Strain Name:
C57BL/6J-MtgxR4416Btlr/Mmmh
Stock Number:
041137-MU
Citation ID:
RRID:MMRRC_041137-MU
Other Names:
R4416 (G1), C57BL/6J-MtgxR4416Btlr
Major Collection:

Strain Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: RPC5, Sin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26939
Homologene: 14052
Icos
Name: inducible T cell co-stimulator
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54167
HGNC: HGNC:5351
Homologene: 8097
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: AS3, Aprin
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100710
Homologene: 41001
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Srp9
Name: signal recognition particle 9
Synonyms: 9kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 27058
Homologene: 37737
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: 5830453M24Rik, Pdzgef1, RA-GEF-1, CNRasGEF, nRapGEP
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 76089
Homologene: 35477
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,994,690 bp
  • A to T, chromosome 1 at 182,131,411 bp
  • A to T, chromosome 1 at 185,282,347 bp
  • T to C, chromosome 1 at 188,356,874 bp
  • A to G, chromosome 2 at 32,708,072 bp
  • C to T, chromosome 2 at 40,663,667 bp
  • G to T, chromosome 2 at 44,575,590 bp
  • G to T, chromosome 2 at 69,527,231 bp
  • T to C, chromosome 3 at 32,461,530 bp
  • A to T, chromosome 3 at 37,405,229 bp
  • A to T, chromosome 3 at 66,061,185 bp
  • C to A, chromosome 3 at 79,069,057 bp
  • T to C, chromosome 3 at 96,658,246 bp
  • T to C, chromosome 4 at 41,505,574 bp
  • A to G, chromosome 4 at 121,054,678 bp
  • T to G, chromosome 4 at 155,587,726 bp
  • A to G, chromosome 5 at 36,471,272 bp
  • A to G, chromosome 5 at 87,558,576 bp
  • T to C, chromosome 5 at 139,972,194 bp
  • C to T, chromosome 5 at 150,736,396 bp
  • T to A, chromosome 6 at 17,339,249 bp
  • G to T, chromosome 6 at 97,178,988 bp
  • G to A, chromosome 6 at 115,935,230 bp
  • A to G, chromosome 7 at 35,416,397 bp
  • G to T, chromosome 7 at 81,968,960 bp
  • G to A, chromosome 7 at 106,689,011 bp
  • T to C, chromosome 7 at 120,939,057 bp
  • G to T, chromosome 9 at 27,322,917 bp
  • A to G, chromosome 9 at 39,942,428 bp
  • A to T, chromosome 10 at 14,129,170 bp
  • T to C, chromosome 10 at 59,428,103 bp
  • T to C, chromosome 10 at 62,659,569 bp
  • T to C, chromosome 10 at 129,873,957 bp
  • A to T, chromosome 11 at 87,035,288 bp
  • T to A, chromosome 12 at 70,877,249 bp
  • C to G, chromosome 12 at 87,081,902 bp
  • A to G, chromosome 14 at 68,622,082 bp
  • T to A, chromosome 15 at 60,016,423 bp
  • T to A, chromosome 16 at 15,648,757 bp
  • C to A, chromosome 16 at 88,051,461 bp
  • G to A, chromosome 17 at 45,566,429 bp
  • T to C, chromosome 19 at 40,038,259 bp
  • T to A, chromosome 19 at 45,040,580 bp
  • T to G, chromosome 19 at 55,380,948 bp
  • G to T, chromosome 19 at 57,385,397 bp
  • G to A, chromosome X at 71,939,619 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4416 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041137-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.