Strain Name:
C57BL/6J-MtgxR4416Btlr/Mmmh
Stock Number:
041137-MU
Citation ID:
RRID:MMRRC_041137-MU
Other Names:
R4416 (G1), C57BL/6J-MtgxR4416Btlr
Major Collection:

Gene Information

Polr3e
Name: polymerase (RNA) III (DNA directed) polypeptide E
Synonyms: Sin, RPC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 26939
Homologene: 14052
Icos
Name: inducible T cell co-stimulator
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 54167
HGNC: HGNC:5351
Homologene: 8097
Pds5b
Name: PDS5 cohesin associated factor B
Synonyms: WAP-Her-2, Aprin, AS3, Her-2 Tg, Tg(Wap-ERBB2)229Wzw
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100710
Homologene: 41001
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, D230004K18Rik, b2b1625.2Clo, Gp330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PK, XRCC7, dxnph, DNAPDcs, slip, DOXNPH, DNA-PKcs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Srp9
Name: signal recognition particle 9
Synonyms: 9kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27058
Homologene: 37737
Rapgef2
Name: Rap guanine nucleotide exchange factor (GEF) 2
Synonyms: CNRasGEF, RA-GEF-1, nRapGEP, 5830453M24Rik, Pdzgef1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 76089
Homologene: 35477
Rab3gap2
Name: RAB3 GTPase activating protein subunit 2
Synonyms: 1110059F07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98732
Homologene: 40842
Vti1a
Name: vesicle transport through interaction with t-SNAREs 1A
Synonyms: 4921537J05Rik, 1110018K19Rik, 1110014F16Rik, Vti1-rp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 53611
VEGA: 19
Homologene: 39963
Rho
Name: rhodopsin
Synonyms: Rod Opsin, Long Wavelength Sensitive opsin, opsin 2, RP4, Opn2, L opsin, LWS opsin, Ops, Noerg1, Red Opsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 212541
Homologene: 68068
Oit3
Name: oncoprotein induced transcript 3
Synonyms: EF-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18302
Homologene: 7870
Grpel1
Name: GrpE-like 1, mitochondrial
Synonyms: mt-GrpE#1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17713
Homologene: 6992
Nadk
Name: NAD kinase
Synonyms: 4432404C02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 192185
Homologene: 49724
Frmd6
Name: FERM domain containing 6
Synonyms: 4930488L10Rik, 2610019M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 319710
VEGA: 12
Homologene: 12449
Elfn1
Name: leucine rich repeat and fibronectin type III, extracellular 1
Synonyms: A930017N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243312
Homologene: 18465
Col9a2
Name: collagen, type IX, alpha 2
Synonyms: Col9a-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12840
HGNC: HGNC:2218
Homologene: 37535
Fam160b1
Name: family with sequence similarity 160, member B1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226252
VEGA: 19
Homologene: 28133
Ush2a
Name: usherin
Synonyms: MUSH2A, A930037M10Rik, LOC381317, Ush2a, Ushrn, A930011D15Rik, LOC269160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22283
Homologene: 66151
Olfr974
Name: olfactory receptor 974
Synonyms: GA_x6K02T2PVTD-33640290-33641222, MOR171-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 259111
VEGA: 9
Homologene: 133680
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: Shn-2, Schnurri-2, Gm20114, MIBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Slc35b2
Name: solute carrier family 35, member B2
Synonyms: PAPST1, 1110003M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73836
VEGA: 17
Homologene: 24504
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Pik3ca
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Synonyms: p110alpha, caPI3K, 6330412C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18706
HGNC: HGNC:8975
Homologene: 21249
Bnc1
Name: basonuclin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12173
HGNC: HGNC:1081
Homologene: 31048
Grik1
Name: glutamate receptor, ionotropic, kainate 1
Synonyms: GluK5, Glur-5, A830007B11Rik, Glurbeta1, D16Ium24e, D16Ium24, Glur5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14805
HGNC: HGNC:4579
Homologene: 68992
Cyp2c54
Name: cytochrome P450, family 2, subfamily c, polypeptide 54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 404195
VEGA: 19
Homologene: 137231
Cav1
Name: caveolin 1, caveolae protein
Synonyms: Cav-1, caveolin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12389
HGNC: HGNC:1527
Homologene: 1330
Gtdc1
Name: glycosyltransferase-like domain containing 1
Synonyms: E330008O22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227835
Homologene: 32590
Cep89
Name: centrosomal protein 89
Synonyms: 2610507L03Rik, Ccdc123
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72140
Homologene: 12444
Igsf9b
Name: immunoglobulin superfamily, member 9B
Synonyms: LOC235086
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235086
Homologene: 19472
Gm1141
Name: predicted gene 1141
Synonyms: Pasd1, LOC382221
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 382221
Itga10
Name: integrin, alpha 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 213119
HGNC: HGNC:6135
Homologene: 2697
Tmf1
Name: TATA element modulatory factor 1
Synonyms: LOC232286, 7030402D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232286
Homologene: 133801
Adam28
Name: a disintegrin and metallopeptidase domain 28
Synonyms: MDC-L, Dtgn1, D430033C21Rik, C130072N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13522
VEGA: 14
HGNC: HGNC:206
Homologene: 40705
Pdzd7
Name: PDZ domain containing 7
Synonyms: Pdzk7, EG435601
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 100503041
Homologene: 129509
Olfr694
Name: olfactory receptor 694
Synonyms: MOR283-9, GA_x6K02T2PBJ9-9067220-9066273
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258444
Homologene: 79345
Tmem63c
Name: transmembrane protein 63c
Synonyms: 9330187M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217733
Homologene: 33129
Sult1d1
Name: sulfotransferase family 1D, member 1
Synonyms: tyrosine-ester sulfotransferase, Sultn, 5033411P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53315
Homologene: 124464
Olfr814
Name: olfactory receptor 814
Synonyms: GA_x6K02T2PULF-11553313-11552381, MOR113-8, MOR113-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 259165
Homologene: 133585
1110017D15Rik
Name: RIKEN cDNA 1110017D15 gene
Synonyms: Smrp1, Cbe1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73721
Homologene: 13074
Veph1
Name: ventricular zone expressed PH domain-containing 1
Synonyms: 2810471M23Rik, Veph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72789
Homologene: 11624
Cdk9
Name: cyclin-dependent kinase 9 (CDC2-related kinase)
Synonyms: PITALRE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 107951
HGNC: HGNC:1780
Homologene: 55566
4933412E24Rik
Name: RIKEN cDNA 4933412E24 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71088
VEGA: 15
Homologene: 129990
Nudt6
Name: nudix (nucleoside diphosphate linked moiety X)-type motif 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229228
HGNC: HGNC:8053
Homologene: 31425
Gdpd1
Name: glycerophosphodiester phosphodiesterase domain containing 1
Synonyms: 2610020H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 66569
Homologene: 7069
Stox1
Name: storkhead box 1
Synonyms: 4732470K04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216021
Homologene: 17649
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 60,994,690 bp
  • A to T, chromosome 1 at 182,131,411 bp
  • A to T, chromosome 1 at 185,282,347 bp
  • T to C, chromosome 1 at 188,356,874 bp
  • A to G, chromosome 2 at 32,708,072 bp
  • C to T, chromosome 2 at 40,663,667 bp
  • G to T, chromosome 2 at 44,575,590 bp
  • G to T, chromosome 2 at 69,527,231 bp
  • T to C, chromosome 3 at 32,461,530 bp
  • A to T, chromosome 3 at 37,405,229 bp
  • A to T, chromosome 3 at 66,061,185 bp
  • C to A, chromosome 3 at 79,069,057 bp
  • T to C, chromosome 3 at 96,658,246 bp
  • T to C, chromosome 4 at 41,505,574 bp
  • A to G, chromosome 4 at 121,054,678 bp
  • T to G, chromosome 4 at 155,587,726 bp
  • A to G, chromosome 5 at 36,471,272 bp
  • A to G, chromosome 5 at 87,558,576 bp
  • T to C, chromosome 5 at 139,972,194 bp
  • C to T, chromosome 5 at 150,736,396 bp
  • T to A, chromosome 6 at 17,339,249 bp
  • G to T, chromosome 6 at 97,178,988 bp
  • G to A, chromosome 6 at 115,935,230 bp
  • A to G, chromosome 7 at 35,416,397 bp
  • G to T, chromosome 7 at 81,968,960 bp
  • G to A, chromosome 7 at 106,689,011 bp
  • T to C, chromosome 7 at 120,939,057 bp
  • G to T, chromosome 9 at 27,322,917 bp
  • A to G, chromosome 9 at 39,942,428 bp
  • A to T, chromosome 10 at 14,129,170 bp
  • T to C, chromosome 10 at 59,428,103 bp
  • T to C, chromosome 10 at 62,659,569 bp
  • T to C, chromosome 10 at 129,873,957 bp
  • A to T, chromosome 11 at 87,035,288 bp
  • T to A, chromosome 12 at 70,877,249 bp
  • C to G, chromosome 12 at 87,081,902 bp
  • A to G, chromosome 14 at 68,622,082 bp
  • T to A, chromosome 15 at 60,016,423 bp
  • T to A, chromosome 16 at 15,648,757 bp
  • C to A, chromosome 16 at 88,051,461 bp
  • G to A, chromosome 17 at 45,566,429 bp
  • T to C, chromosome 19 at 40,038,259 bp
  • T to A, chromosome 19 at 45,040,580 bp
  • T to G, chromosome 19 at 55,380,948 bp
  • G to T, chromosome 19 at 57,385,397 bp
  • G to A, chromosome X at 71,939,619 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4416 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041137-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.