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Strain Name:
C57BL/6J-MtgxR4418Btlr/Mmmh
Stock Number:
041139-MU
Citation ID:
RRID:MMRRC_041139-MU
Other Names:
R4418 (G1), C57BL/6J-MtgxR4418Btlr
Major Collection:

Strain Information

Gpr6
Name: G protein-coupled receptor 6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 140741
VEGA: 10
HGNC: HGNC:4515
Homologene: 38026
Pecam1
Name: platelet/endothelial cell adhesion molecule 1
Synonyms: PECAM-1, Cd31
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18613
HGNC: HGNC:8823
Homologene: 47925
Dgka
Name: diacylglycerol kinase, alpha
Synonyms: Dagk1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13139
VEGA: 10
HGNC: HGNC:2849
Homologene: 1028
Mrpl39
Name: mitochondrial ribosomal protein L39
Synonyms: MRP-L5, Rpml5
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 27393
Homologene: 9679
Dclre1c
Name: DNA cross-link repair 1C
Synonyms: 9930121L06Rik, Artemis, Art
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227525
Homologene: 32547
Col6a1
Name: collagen, type VI, alpha 1
Synonyms: Col6a-1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12833
HGNC: HGNC:2211
Homologene: 1391
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,104,027 bp
  • A to G, chromosome 1 at 160,044,802 bp
  • G to A, chromosome 1 at 189,256,727 bp
  • T to A, chromosome 1 at 191,348,432 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • T to C, chromosome 2 at 3,452,935 bp
  • A to G, chromosome 2 at 28,498,408 bp
  • A to T, chromosome 2 at 76,889,481 bp
  • A to T, chromosome 2 at 104,789,421 bp
  • C to T, chromosome 2 at 112,831,224 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • A to T, chromosome 2 at 125,752,915 bp
  • A to G, chromosome 2 at 150,118,185 bp
  • C to A, chromosome 2 at 167,105,675 bp
  • A to G, chromosome 3 at 3,648,094 bp
  • G to A, chromosome 3 at 106,203,727 bp
  • A to G, chromosome 4 at 115,009,377 bp
  • T to C, chromosome 4 at 145,536,728 bp
  • T to C, chromosome 5 at 69,341,162 bp
  • A to T, chromosome 5 at 150,048,800 bp
  • G to A, chromosome 6 at 29,379,424 bp
  • C to A, chromosome 6 at 128,645,159 bp
  • A to G, chromosome 7 at 131,222,708 bp
  • A to T, chromosome 7 at 131,247,465 bp
  • A to T, chromosome 7 at 141,016,071 bp
  • A to T, chromosome 7 at 143,709,815 bp
  • G to A, chromosome 8 at 33,617,049 bp
  • T to C, chromosome 8 at 61,106,864 bp
  • T to C, chromosome 8 at 71,693,769 bp
  • A to T, chromosome 8 at 94,405,293 bp
  • C to A, chromosome 8 at 109,530,633 bp
  • T to A, chromosome 9 at 55,838,180 bp
  • C to T, chromosome 9 at 58,843,895 bp
  • G to T, chromosome 9 at 88,582,153 bp
  • T to A, chromosome 9 at 114,706,170 bp
  • C to T, chromosome 10 at 40,936,582 bp
  • T to G, chromosome 10 at 41,070,608 bp
  • T to A, chromosome 10 at 76,718,405 bp
  • G to T, chromosome 10 at 78,217,188 bp
  • T to G, chromosome 10 at 127,091,650 bp
  • T to G, chromosome 10 at 127,278,365 bp
  • A to T, chromosome 10 at 128,728,094 bp
  • A to G, chromosome 11 at 49,261,245 bp
  • A to C, chromosome 11 at 60,468,146 bp
  • T to A, chromosome 11 at 70,647,917 bp
  • C to T, chromosome 11 at 94,567,837 bp
  • A to T, chromosome 11 at 96,940,969 bp
  • T to C, chromosome 11 at 103,618,314 bp
  • A to G, chromosome 11 at 106,695,922 bp
  • C to T, chromosome 12 at 69,307,597 bp
  • G to A, chromosome 12 at 70,978,753 bp
  • C to T, chromosome 13 at 23,684,503 bp
  • A to G, chromosome 13 at 73,684,395 bp
  • A to G, chromosome 13 at 93,402,069 bp
  • C to T, chromosome 13 at 100,300,600 bp
  • A to G, chromosome 14 at 11,227,879 bp
  • T to A, chromosome 14 at 43,748,845 bp
  • G to T, chromosome 14 at 51,418,081 bp
  • A to G, chromosome 14 at 52,136,754 bp
  • T to G, chromosome 14 at 120,943,893 bp
  • T to A, chromosome 15 at 9,279,980 bp
  • C to A, chromosome 16 at 84,725,124 bp
  • A to T, chromosome 17 at 20,583,689 bp
  • T to A, chromosome 17 at 23,644,070 bp
  • T to A, chromosome 17 at 34,188,379 bp
  • C to T, chromosome 18 at 37,343,482 bp
  • T to C, chromosome 18 at 38,261,287 bp
  • T to C, chromosome 18 at 86,404,856 bp
  • T to C, chromosome 19 at 23,979,435 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4418 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041139-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.