Strain Name:
C57BL/6J-MtgxR4420Btlr/Mmmh
Stock Number:
041141-MU
Citation ID:
RRID:MMRRC_041141-MU
Other Names:
R4420 (G1), C57BL/6J-MtgxR4420Btlr
Major Collection:

Gene Information

Dclre1c
Name: DNA cross-link repair 1C
Synonyms: 9930121L06Rik, Artemis, Art
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227525
Homologene: 32547
Matk
Name: megakaryocyte-associated tyrosine kinase
Synonyms: Ntk, HYL, CHK, Csk homologous kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17179
HGNC: HGNC:6906
Homologene: 48104
Elp3
Name: elongator acetyltransferase complex subunit 3
Synonyms: 2610507P14Rik, KAT9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74195
VEGA: 14
Homologene: 7105
Trip10
Name: thyroid hormone receptor interactor 10
Synonyms: Cip4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106628
VEGA: 17
Homologene: 99728
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Psme4
Name: proteasome (prosome, macropain) activator subunit 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103554
Homologene: 113742
Fbrsl1
Name: fibrosin-like 1
Synonyms: LOC381668, 2410025L10Rik, Gm29766
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381668
Homologene: 45966
Zmym2
Name: zinc finger, MYM-type 2
Synonyms: SCLL, RAMP, MYM, FIM, Zfp198
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76007
VEGA: 14
Homologene: 12631
Atp1b1
Name: ATPase, Na+/K+ transporting, beta 1 polypeptide
Synonyms: sodium/potassium ATPase beta subunit, Atpb-1, Atpb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 11931
HGNC: HGNC:804
Homologene: 37509
Hus1
Name: HUS1 checkpoint clamp component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15574
HGNC: HGNC:5309
Homologene: 37932
Jcad
Name: junctional cadherin 5 associated
Synonyms: 9430020K01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240185
VEGA: 18
Homologene: 19370
Casz1
Name: castor zinc finger 1
Synonyms: 2410019P08Rik, D4Ertd432e, castor, Cst
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69743
Homologene: 9824
Nceh1
Name: neutral cholesterol ester hydrolase 1
Synonyms: CPO-BP, mKIAA1363, B230106I24Rik, Aadacl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 320024
Homologene: 23251
Samd12
Name: sterile alpha motif domain containing 12
Synonyms: A830094I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320679
Homologene: 35979
Tas1r3
Name: taste receptor, type 1, member 3
Synonyms: T1r3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 83771
Homologene: 12890
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Tarbp1
Name: TAR RNA binding protein 1
Synonyms: Gm17296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 212728
Homologene: 38082
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: 3110001A05Rik, 1810064L21Rik, 3110027P15Rik, A230065J02Rik, Ugcgl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66435
Homologene: 56875
Chp2
Name: calcineurin-like EF hand protein 2
Synonyms: Cbhp2, 2010110P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 70261
Homologene: 11131
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Pcdh7
Name: protocadherin 7
Synonyms: BH-protocadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54216
HGNC: HGNC:8659
Homologene: 36101
Wdr95
Name: WD40 repeat domain 95
Synonyms: 4930434E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381693
Homologene: 124480
Il12rb2
Name: interleukin 12 receptor, beta 2
Synonyms: IL-12RB2, Ifnm, A930027I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16162
HGNC: HGNC:5972
Homologene: 1197
Slc35f4
Name: solute carrier family 35, member F4
Synonyms: 4930550L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75288
Homologene: 45939
Hsf5
Name: heat shock transcription factor family member 5
Synonyms: LOC327992
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327992
Homologene: 52701
Irs1
Name: insulin receptor substrate 1
Synonyms: IRS-1, G972R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16367
HGNC: HGNC:6125
Homologene: 4049
Dnah9
Name: dynein, axonemal, heavy chain 9
Synonyms: D11Ertd686e, Dnahc9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237806
HGNC: HGNC:2953
Homologene: 20357
Gzmn
Name: granzyme N
Synonyms: GrN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 245839
VEGA: 14
Homologene: 87221
Carmil3
Name: capping protein regulator and myosin 1 linker 3
Synonyms: Lrrc16b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 268747
VEGA: 14
Homologene: 74564
Sugct
Name: succinyl-CoA glutarate-CoA transferase
Synonyms: 5033411D12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 192136
VEGA: 13
Homologene: 11681
Duox1
Name: dual oxidase 1
Synonyms: LNOX1, 9930101G15Rik, NOXEF1, THOX1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99439
HGNC: HGNC:3062
Homologene: 68136
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218214
Homologene: 14695
Heg1
Name: heart development protein with EGF-like domains 1
Synonyms: LOC268884, 5530401I02Rik, 9530025L16Rik, 4632417D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 77446
Homologene: 35276
Smc1b
Name: structural maintenance of chromosomes 1B
Synonyms: SMC1beta, Smc1l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 140557
VEGA: 15
Homologene: 13786
Gucy1a2
Name: guanylate cyclase 1, soluble, alpha 2
Synonyms: 6330407I18Rik, A230060L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234889
HGNC: HGNC:4684
Homologene: 47953
Mroh5
Name: maestro heat-like repeat family member 5
Synonyms: LOC268816, Gm628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268816
Traf3ip3
Name: TRAF3 interacting protein 3
Synonyms: 6030423D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 215243
Homologene: 11885
Ppfibp1
Name: PTPRF interacting protein, binding protein 1 (liprin beta 1)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 67533
HGNC: HGNC:9249
Homologene: 2685
Zc3h15
Name: zinc finger CCCH-type containing 15
Synonyms: FM22, Ierepo4, 1810012H02Rik, 1700006A17Rik, 2610312B22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 69082
Homologene: 10216
Spata2l
Name: spermatogenesis associated 2-like
Synonyms: 2610039E05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 78779
Homologene: 32729
Zfp763
Name: zinc finger protein 763
Synonyms: 1700065O13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 73451
Homologene: 66274
Tas2r117
Name: taste receptor, type 2, member 117
Synonyms: Tas2r17, mGR17, T2R17, mt2r54
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 353166
Homologene: 130073
Olfr1086
Name: olfactory receptor 1086
Synonyms: GA_x6K02T2Q125-48168771-48167839, MOR179-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258585
Homologene: 73975
Gnat3
Name: guanine nucleotide binding protein, alpha transducing 3
Synonyms: Ggust, Gtn, alpha-gustducin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 242851
Homologene: 24284
Chfr
Name: checkpoint with forkhead and ring finger domains
Synonyms: RNF116, 5730484M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231600
Homologene: 10078
Pla2g4d
Name: phospholipase A2, group IVD
Synonyms: 2610311B01Rik, Pla2delta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 78390
Homologene: 52252
Hoxb9
Name: homeobox B9
Synonyms: Hox-2.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15417
HGNC: HGNC:5120
Homologene: 7367
Prdx5
Name: peroxiredoxin 5
Synonyms: peroxiredoxin V, PrxV, PrxV, PMP20, Prdx6, AOEB166, AOPP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 54683
HGNC: HGNC:9355
Homologene: 8076
Nqo1
Name: NAD(P)H dehydrogenase, quinone 1
Synonyms: NMO1, NQO1, QR1, NAD(P)H dehydrogenase (quinone), Ox-1, Ox1, Nmor1, Dia4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 18104
HGNC: HGNC:2874
Homologene: 695
Gm15931
Name: predicted gene 15931
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 82,288,450 bp
  • T to C, chromosome 1 at 164,453,558 bp
  • T to C, chromosome 1 at 193,197,777 bp
  • A to T, chromosome 2 at 3,433,745 bp
  • C to A, chromosome 2 at 83,658,012 bp
  • A to G, chromosome 2 at 86,676,919 bp
  • A to G, chromosome 2 at 120,284,163 bp
  • G to A, chromosome 2 at 122,327,126 bp
  • A to G, chromosome 3 at 27,241,649 bp
  • T to G, chromosome 4 at 148,948,918 bp
  • A to G, chromosome 4 at 155,862,332 bp
  • T to C, chromosome 5 at 17,999,801 bp
  • T to C, chromosome 5 at 58,129,170 bp
  • A to C, chromosome 5 at 101,910,984 bp
  • T to A, chromosome 5 at 110,170,880 bp
  • T to C, chromosome 5 at 110,378,986 bp
  • G to A, chromosome 5 at 149,532,666 bp
  • G to T, chromosome 6 at 67,316,410 bp
  • A to G, chromosome 6 at 73,191,479 bp
  • T to A, chromosome 6 at 132,803,349 bp
  • T to A, chromosome 6 at 147,026,238 bp
  • T to C, chromosome 7 at 4,281,277 bp
  • GGCCAGC to G, chromosome 7 at 80,098,349 bp
  • T to C, chromosome 7 at 122,221,938 bp
  • C to T, chromosome 8 at 107,392,117 bp
  • T to C, chromosome 8 at 123,234,029 bp
  • G to T, chromosome 8 at 126,447,080 bp
  • T to A, chromosome 9 at 3,634,640 bp
  • A to G, chromosome 10 at 81,262,457 bp
  • T to C, chromosome 11 at 9,000,133 bp
  • C to T, chromosome 11 at 30,812,028 bp
  • T to C, chromosome 11 at 66,118,749 bp
  • T to G, chromosome 11 at 87,657,304 bp
  • T to C, chromosome 11 at 96,271,981 bp
  • C to T, chromosome 13 at 17,452,545 bp
  • C to T, chromosome 13 at 47,063,077 bp
  • A to T, chromosome 14 at 49,313,577 bp
  • A to G, chromosome 14 at 55,493,588 bp
  • A to T, chromosome 14 at 56,166,006 bp
  • G to A, chromosome 14 at 56,956,878 bp
  • G to A, chromosome 14 at 65,580,791 bp
  • A to T, chromosome 14 at 119,009,007 bp
  • G to A, chromosome 15 at 53,860,259 bp
  • TGGAG to TG, chromosome 15 at 73,783,074 bp
  • A to G, chromosome 15 at 85,112,830 bp
  • A to T, chromosome 16 at 33,727,435 bp
  • T to G, chromosome 17 at 33,018,481 bp
  • C to T, chromosome 17 at 57,255,448 bp
  • T to C, chromosome 18 at 4,676,032 bp
  • C to A, chromosome 19 at 6,907,964 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4420 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041141-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.