Strain Name:
C57BL/6J-MtgxR4437Btlr/Mmmh
Stock Number:
041150-MU
Citation ID:
RRID:MMRRC_041150-MU
Other Names:
R4437 (G1), C57BL/6J-MtgxR4437Btlr
Major Collection:

Gene Information

Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77573
Homologene: 11294
Itga6
Name: integrin alpha 6
Synonyms: Cd49f, 5033401O05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16403
HGNC: HGNC:6142
Homologene: 20091
Npr1
Name: natriuretic peptide receptor 1
Synonyms: NPRA, GC-A, NPR-A, guanylyl cyclase-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18160
HGNC: HGNC:7943
Homologene: 37367
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72313
Homologene: 103956
Thbs3
Name: thrombospondin 3
Synonyms: Thbs-3, TSP3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 21827
Homologene: 5159
Fam169a
Name: family with sequence similarity 169, member A
Synonyms: B230112C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320557
VEGA: 13
Homologene: 52656
Cdc37l1
Name: cell division cycle 37-like 1
Synonyms: 2700033A15Rik, Hsp90-associating relative of Cdc37, Harc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 67072
VEGA: 19
Homologene: 9912
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: 9430093H17Rik, Falz
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Pvr
Name: poliovirus receptor
Synonyms: mE4, 3830421F03Rik, Taa1, CD155, Tage4, necl-5, D7Ertd458e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 52118
Homologene: 79541
Siah1b
Name: siah E3 ubiquitin protein ligase 1B
Synonyms: Sinh1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 20438
Homologene: 56441
Camsap1
Name: calmodulin regulated spectrin-associated protein 1
Synonyms: PRO2405, 9530003A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227634
Homologene: 19202
Robo2
Name: roundabout guidance receptor 2
Synonyms: 9430089E08Rik, D230004I22Rik, 2600013A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 268902
Homologene: 43188
Bcar1
Name: breast cancer anti-estrogen resistance 1
Synonyms: p130Cas, Cas, Crkas
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12927
HGNC: HGNC:971
Homologene: 7674
Pfas
Name: phosphoribosylformylglycinamidine synthase (FGAR amidotransferase)
Synonyms: 4432409B16Rik, Sofa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237823
HGNC: HGNC:8863
Homologene: 5970
Gm2381
Name: predicted gene 2381
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100039706
Trerf1
Name: transcriptional regulating factor 1
Synonyms: Trep132, Trep-132, 9430096I18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224829
Homologene: 14129
Caprin2
Name: caprin family member 2
Synonyms: Eeg1, C1qdc1, RNG140
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232560
Homologene: 11393
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberin, Nafld, tuberous sclerosis 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: MIBP1, Schnurri-2, Shn-2, Gm20114
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Cul9
Name: cullin 9
Synonyms: 1810035I07Rik, Parc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78309
Homologene: 56696
Gm4884
Name: predicted gene 4884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233164
AW549877
Name: expressed sequence AW549877
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 106064
VEGA: 15
Homologene: 27085
Myoc
Name: myocilin
Synonyms: TIGR, GLC1A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 17926
HGNC: HGNC:7610
Homologene: 220
Col4a1
Name: collagen, type IV, alpha 1
Synonyms: Col4a-1, Del(8)Bru44H, Del(8)44H, Bru, Svc, Raw, alpha1(IV) collagen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12826
HGNC: HGNC:2202
Homologene: 20437
Pde4dip
Name: phosphodiesterase 4D interacting protein (myomegalin)
Synonyms: D3Bwg1078e, 4732458A06Rik, D130016K21Rik, 9430063L05Rik, Usmg4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 83679
Homologene: 66961
Frem3
Name: Fras1 related extracellular matrix protein 3
Synonyms: LOC333315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333315
Homologene: 35388
Ciao3
Name: cytosolic iron-sulfur assembly component 3
Synonyms: 9030612I22Rik, Narfl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 67563
Homologene: 6750
Mdga2
Name: MAM domain containing glycosylphosphatidylinositol anchor 2
Synonyms: Mdga2, 9330209L04Rik, 6720489L24Rik, Mamdc1, Adp, Tg(Prnp-PFN1*G118V)838Kiaei
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320772
Homologene: 45659
Sgsm3
Name: small G protein signaling modulator 3
Synonyms: 1810012I01Rik, CIP85, Rutbc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105835
Homologene: 9249
D630003M21Rik
Name: RIKEN cDNA D630003M21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228846
Homologene: 18598
Rln1
Name: relaxin 1
Synonyms: rlx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19773
Homologene: 55524
Adam6b
Name: a disintegrin and metallopeptidase domain 6B
Synonyms: 4930523C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238405
Homologene: 128362
Olfr725
Name: olfactory receptor 725
Synonyms: GA_x6K02T2PMLR-5725741-5724776, MOR246-7_p, MOR246-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 258314
Homologene: 44957
Olfr1240
Name: olfactory receptor 1240
Synonyms: GA_x6K02T2Q125-50883183-50882239, MOR231-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258804
Homologene: 121591
Arhgef16
Name: Rho guanine nucleotide exchange factor (GEF) 16
Synonyms: Neuroblastoma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230972
Homologene: 82484
Fam71d
Name: family with sequence similarity 71, member D
Synonyms: 4930516C23Rik, 4921509E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 70897
Homologene: 49887
Bcl2a1d
Name: B cell leukemia/lymphoma 2 related protein A1d
Synonyms: A1-d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12047
HGNC: HGNC:991
Homologene: 2988
Nkx2-4
Name: NK2 homeobox 4
Synonyms: Nkx-2.4, tinman, 1700001P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228731
HGNC: HGNC:7837
Homologene: 41526
Olfr645
Name: olfactory receptor 645
Synonyms: GA_x6K02T2PBJ9-6819097-6818150, MOR13-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258247
Homologene: 66155
Igkv1-99
Name: immunoglobulin kappa variable 1-99
Synonyms: Gm16800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434028
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 162,649,112 bp
  • G to A, chromosome 2 at 25,938,646 bp
  • T to C, chromosome 2 at 71,825,638 bp
  • T to G, chromosome 2 at 89,439,354 bp
  • T to C, chromosome 2 at 147,084,259 bp
  • G to A, chromosome 2 at 158,213,462 bp
  • T to A, chromosome 3 at 89,217,675 bp
  • T to G, chromosome 3 at 90,456,286 bp
  • G to A, chromosome 3 at 97,766,569 bp
  • A to G, chromosome 4 at 154,279,696 bp
  • G to A, chromosome 5 at 73,119,814 bp
  • A to T, chromosome 5 at 123,531,884 bp
  • T to G, chromosome 6 at 68,542,334 bp
  • A to G, chromosome 6 at 148,848,145 bp
  • T to A, chromosome 7 at 19,910,795 bp
  • A to G, chromosome 7 at 41,043,090 bp
  • A to T, chromosome 7 at 42,819,844 bp
  • T to C, chromosome 7 at 104,084,921 bp
  • G to T, chromosome 8 at 11,206,387 bp
  • A to G, chromosome 8 at 80,612,607 bp
  • G to A, chromosome 8 at 111,715,389 bp
  • A to T, chromosome 9 at 88,731,700 bp
  • C to A, chromosome 10 at 14,128,969 bp
  • A to G, chromosome 11 at 68,988,417 bp
  • A to G, chromosome 11 at 107,074,474 bp
  • T to C, chromosome 12 at 66,473,198 bp
  • T to A, chromosome 12 at 78,715,050 bp
  • A to C, chromosome 12 at 113,490,661 bp
  • A to G, chromosome 13 at 97,126,740 bp
  • T to C, chromosome 14 at 50,034,830 bp
  • T to C, chromosome 15 at 3,991,836 bp
  • T to C, chromosome 15 at 81,002,591 bp
  • T to C, chromosome 16 at 73,973,244 bp
  • G to A, chromosome 17 at 24,599,713 bp
  • C to A, chromosome 17 at 25,781,797 bp
  • A to G, chromosome 17 at 46,502,159 bp
  • A to G, chromosome 17 at 47,359,034 bp
  • T to C, chromosome 19 at 29,007,621 bp
  • A to T, chromosome 19 at 29,334,562 bp
  • G to A, chromosome X at 164,071,692 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4437 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041150-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.