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Hemizygous: Gastric epithelia cell expression of Lewis B BabA-mediated attachment to gastric epithelial cells by Helicobacter pylori in histosections
Homozygous: Gastric epithelia cell expression of Lewis B BabA-mediated attachment to gastric epithelial cells by Helicobacter pylori in histosections
A 1103-bp a-1,3/4-fucosyltransferase DNA which includes a 1086-nt coding region specifying the 361-aa type II transmembrane glycoprotein, an upstream Kozak consensus sequence and HindIII site, and a downstream Not I recognition site was recovered by using PCR and a human placental cDNA library. The 1.1-kb HindIII-Not I restriction fragment was excised, incubated with Klenow DNA polymerase to fill in the ends, and then subcloned into pLPNAB2 which had been treated previously with BamHI and Klenow enzyme. pLPNAB2 contains nt -596 to +21 of the rat liver fatty acid-binding protein gene [L-FABP(-596 to +21)] linked to nt +3 to +2150 of the human growth hormone (hGH) gene. The subcloning allowed the a-1,3/4-fucosyltransferase cDNA to be placed downstream of L-FABP(-596 to +21) in the BamHI site of exon I of the hGH gene, creating pLPNAB2al,3/4FT. The 4.0-kb L-FABP(-596 to +21)-al,3/4FT-hGH insert was removed from pLPNAB2al,3/4FT by EcoRI digestion and injected into fertilized FVB/N mouse oocytes. A founder male was used to establish a line of mice that contained three copies of the transgene per haploid genome. The pedigree was maintained by crosses to normal FVB/N littermates.
Falk PG, Bry L, Holgersson J, Gordon JI. Expression of a human a-1,3/4-fucosyltransferase in the pit celllineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates: A potential transgenic mouse model for studying Helicobacter pylori infection. Proc Natl Acad Sci USA. 1995 Feb 28;92(5):1515-9. (Medline PMID: 7878011)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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