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Strain Detail Sheet

Published: 07/28/2019
Revised: 08/07/2025

Strain Name:
CD10.Cg-Tg(ERE-TK,lacZ)6Nagl/Mmmh
Stock Number:
041182-MU
Citation ID:
RRID:MMRRC_041182-MU
Other Names:
ERIN-CD10, EC

Strain Information

Gene Details

lacZ
Name: beta galactosidase
Type: Gene
Species: Escherichia coli (bacterium)
Chromosome:
TK1
Name: thymidine kinase 1, soluble
Type: Gene
Species: Homo sapiens (human)
Chromosome: 17
ERE
Name: estrogen response element
Type: Gene
Species: Homo sapiens (human)
Chromosome:
Tg(ERE-TK,lacZ)6Nagl
Name: transgene insertion 6, Susan Nagel
Synonyms: 3XERE-TK-lacZ
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations

A 3xERE-TK-lacZ construct, consisting of three copies of the vitellogenin estrogen response element (ERE) linked to a minimal TK promoter, followed by a lacZ gene, was used to create transgenic mice. Founder 6 showed broad expression

Genotype Determination

Strain Description

Phenotype

Homozygous: ER reporter gene phenotype for homozygous mice is ~twice the expression of the heterozygous mice

Hemizygous: ER reporter gene phenotype for homozygous mice is ~twice the expression of the heterozygous mice

MeSH Terms
  • 3T3 Cells
  • Animals
  • Benzhydryl Compounds
  • Diethylstilbestrol/pharmacology
  • Estrogen Antagonists/pharmacology
  • Estrogens/pharmacology
  • Estrogens/physiology
  • Estrogens, Non-Steroidal/pharmacology
  • Female
  • Gene Expression
  • Genes, Reporter/drug effects
  • Ligands
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic/genetics
  • Organ Size/drug effects
  • Phenols/pharmacology
  • Receptors, Estrogen/drug effects
  • Receptors, Estrogen/genetics
  • Receptors, Estrogen/metabolism
  • Selective Estrogen Receptor Modulators/pharmacology
  • Tamoxifen/pharmacology
  • Tissue Distribution
  • Uterus/anatomy & histology
  • Xenobiotics/pharmacology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
  • Research Tools

Strain Origin

Submitter
Susan Nagel, Ph.D., University of Missouri.
Primary Reference

Nagel SC, Hagelbarger JL, McDonnell DP. Development of an ER Action Indicator Mouse for the Study of Estrogens, Selective ER Modulators (SERMs), and Xenobiotics. Endocrinology. 2001 Nov;142(11):4721-8.(Medline PMID: 11606437)

Strain Development
Founder transgenic mice were created by microinjection of the transgene into B6SJLF1/J zygotes. Founder 6 was bred with C57BL/6J mice. F1 offspring were crossed and F2 transgenics mated with C57BL/6J mice. Offspring were then backcrossed 7 times to CD10/JlsJ.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
White

Breeding

MMRRC Breeding System

Backcross or Sib-mating

Breeding Scheme(s)
  • Backcross
    • Wild-type (CD10/JlsJ) female x Hemizygous male or reciprocal mating
  • Sib-mating:
    • Wild-type female x Hemizygous male littermate or reciprocal mating
    • Hemizygous female x Hemizygous male littermate
    • Homozygous female x Homozygous male littermate
Generation
N7
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months >12 months
Average litter size
10-14
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.