Strain Name:
B6;129P2-Mirc10tm1Mtm/MbpMmucd
Stock Number:
041190-UCD
Citation ID:
RRID:MMRRC_041190-UCD
Other Names:
miR-15b/ miR-16-2
Major Collection:

Strain Information

Mirc10tm1Mtm
Name: microRNA cluster 10, including Mir15b through Mir16-2; targeted mutation 1, Michael McManus
Synonyms: miR-15b/ miR-16-2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Mirc10
Name: microRNA cluster 10, including Mir15b through Mir16-2
Type: Complex/Cluster/Region
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Mir15b
Name: microRNA 15b
Synonyms: Mirn15b, mmu-mir-15b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
NCBI: 387175
VEGA: 3
Mir16-2
Name: microRNA 16-2
Synonyms: mmu-mir-16-2, Mirn16-2, mir-16-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
NCBI: 723949
VEGA: 3
Genetic Alterations
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin cassette, an FRT site and a loxP site (FRT-lacZ-loxP-Neo-FRT-loxP) upstream of the microRNA stem loop, with one loxP site placed immediately downstream of the stem loop.
Genotype Determination
ES Cell Line
E14Tg2a.4 derived from 129P2/OlaHsd, cell line Keck MirKO ES cell line Mirc10
Phenotype

Homozygous: Mice that are homozygous (or heterozygous) for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When combined with a Flp recombinase-expressing strain, the lacZ and neomycin genes are removed leaving an FRT site and the loxP-flanked Mirc10 stem loop. A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mirc10 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP). This mutant mouse strain may be useful in studies of microRNA biology.

Cre-excised Phenotype: A further cross to a Cre-recombinase-expressing strain generates the null allele. When combined with a Cre-recombinase-expressing strain, the neomycin cassette and Mirc10 stem loop are removed leaving a lacZ tagged null allele (FRT-lacZ-loxP).

Mammalian Phenotype Terms
Allelic Composition: Mirc10tm1Mtm/Mirc10tm1Mtm (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

  • phenotype not analyzed
MeSH Terms
  • Animals
  • Embryonic Stem Cells
  • Gene Expression Profiling
  • Genes, Reporter
  • Genotype
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • MicroRNAs/genetics
  • MicroRNAs/metabolism
  • MicroRNAs/physiology
  • Models, Animal
  • Models, Genetic
Strain Development
ES Cell clone Keck MirKO ES cell line Mirc10, MMRRC:036871-UCD, was microinjected into blastocysts, and resulting chimeric mice were crossed with C57BL/6J to derive heterozygous mice.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Research Tools
Donor
MMRRC at University of California, Davis.
Original ES Cell line (MMRRC:036871) donated by Michael McManus, Ph.D., University of California, San Francisco, W.M.Keck Center for Noncoding RNAs
Primary Reference

Park CY, Jeker LT, Carver-Moore K, Oh A, Liu HJ, Cameron R, Richards H, Li Z, Adler D, Yoshinaga Y, Martinez M, Nefadov M, Abbas AK, Weiss A, Lanier LL, de Jong PJ, Bluestone JA, Srivastava D, McManus MT. A Resource for the Conditional Ablation of microRNAs in the Mouse. Cell Rep. 2012 Apr 19;1(4):385-91. (Medline PMID: 22570807).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Varied
MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Wild-type (C57BL/6J) female x Heterozygous male or reciprocal mating
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041190-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
041190-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.