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Strain Name:
B6.129X1-Prkar1atm1Gsm/Mmucd
Stock Number:
000413-UCD
Citation ID:
RRID:MMRRC_000413-UCD
Other Names:
Rialpha, B6.129X1-Prkar1atm1Gsm/Mmcd

Strain Information

Prkar1atm1Gsm
Name: protein kinase, cAMP dependent regulatory, type I, alpha; targeted mutation 1, G Stanley McKnight
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
Prkar1a
Name: protein kinase, cAMP dependent regulatory, type I, alpha
Synonyms: Tse-1, Tse1, 1300018C22Rik, RIalpha
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 19084
HGNC: HGNC:9388
Homologene: 37664
Genetic Alterations
A neo cassette was inserted into exon 3.
Genotype Determination
ES Cell Line
REK3 derived from 129X1/SvJ
Phenotype
Homozygous: Homozygous mutants embryos fail to develop a functional heart tube at E8.5 and are resorbed at approximately E10.5.

Heterozygous: Heterozygous animals are prone to tumor formation and males have defective sperm and on high C57BL/6 background are severely subfertile.
Mammalian Phenotype Terms
Allelic Composition: Prkar1atm1Gsm/Prkar1atm1Gsm (Genetic Background: involves: 129X1/SvJ * C57BL/6 )

Allelic Composition: Prkar1atm1Gsm/Prkar1a+ (Genetic Background: involves: 129X1/SvJ )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Western
  • Catalytic Domain
  • Cell Cycle
  • Cell Movement
  • Chromatography, High Pressure Liquid
  • Crosses, Genetic
  • Cyclic AMP-Dependent Protein Kinases/genetics
  • Cyclic AMP-Dependent Protein Kinases/metabolism
  • Cyclic AMP-Dependent Protein Kinases/physiology
  • Fibroblasts/metabolism
  • Genetic Vectors/metabolism
  • Genotype
  • Heterozygote
  • Homozygote
  • Mesoderm/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Confocal
  • Models, Genetic
  • Molecular Sequence Data
  • Mutation
  • Polymerase Chain Reaction
  • Protein Binding
  • Protein Isoforms
  • Signal Transduction
  • Time Factors
  • Transfection
  • Alleles
  • COS Cells
  • Cercopithecus aethiops
  • Cyclic AMP-Dependent Protein Kinase RIalpha Subunit
  • Cyclic AMP-Dependent Protein Kinases
  • DNA Mutational Analysis
  • Humans
  • Multiple Endocrine Neoplasia/genetics
  • Multiple Endocrine Neoplasia/pathology
  • Myxoma/genetics
  • Myxoma/pathology
  • Pedigree
  • Protein Kinases/genetics
  • Protein Kinases/metabolism
  • Protein Subunits
  • Proteins/genetics
  • Proteins/metabolism
  • Skin Neoplasms/genetics
  • Skin Neoplasms/pathology
  • Spleen/metabolism
  • Spleen/pathology
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Reproduction
Donor
G. Stanley McKnight, Ph.D., University of Washington.
Primary Reference
  • Amieux PS, Howe DG, Knickerbocker H, Lee DC, Su T, Laszlo GS, Idzerda RL, McKnight GS. Increased basal cAMP-dependent protein kinase activity inhibits the formation of mesoderm-derived structures in the developing mouse embryo. J Biol Chem. 2002 Jul 26;277(30):27294-304. Epub 2002 May 9. (Medline PMID: 12004056)
  • Veugelers M, Wilkes D, Burton K, McDermott DA, Song Y, Goldstein MM, La Perle K, Vaughan CJ, O'Hagan A, Bennett KR, Meyer BJ, Legius E, Karttunen M, Norio R, Kaariainen H, Lavyne M, Neau JP, Richter G, Kirali K, Farnsworth A, Stapleton K, Morelli P, Takanashi Y, Bamforth JS, Eitelberger F, Noszian I, Manfroi W, Powers J, Mochizuki Y, Imai T, Ko GT, Driscoll DA, Goldmuntz E, Edelberg JM, Collins A, Eccles D, Irvine AD, McKnight GS, Basson CT. Comparative PRKAR1A genotype-phenotype analyses in humans with Carney complex and prkar1a haploinsufficient mice. Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14222-7. Epub 2004 Sep 15. (Medline PMID: 15371594)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color

Black

Eye
Black
MMRRC Breeding System
Backcross
Generation
N5 (C57BL/6)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes No
Age Reproductive Decline: Unknown Unknown
Average litter size
unknown
Recommended wean age
unknown
Average Pups Weaned
unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Onco-Mouse Patents. Canada: CA1341442 expires Oct. 7, 2020. US patents: 5,925,803 expires July 20, 2016 5,807,571 expired Feb. 11, 2009 4,736,866 expired April 12, 2005

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
000413-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.