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Availability & Fees Order this Strain
Homozygous: lethality in perinatal stages, abnormality in hippocampal electrophysiology
Heterozygous: normal
The targeting vector was introduced into AB2.2 (129S7/SvEvBrd) embryonic stem cells and G418-resistant colonies were selected. Southern blot hybridization confirmed correct integration in two clones, which were expanded and injected into C57BL/6 blastocysts. Resulting chimeras were backcrossed to C57BL/6 for several generations. Heterozygotes from N2 through N5 were sib-mated to generate homozygous mice. Southern blotting and PCR were used for genotyping, and complete absence of Aff4 mRNA containing exon 2 was confirmed by northern blotting, using an exon 2 specific probe.
Note: Aff4 mRNA was detected using other probes, suggesting that there is a truncated mRNA lacking exon 2, even though no protein could be detected using Af5q31 polyclonal antibodies.
Colony Surveillance Program and Current Health Reports
Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.