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Availability & Fees Order this Strain
Homozygous: Undetermined
Heterozygous: Undetermined
Confirmation of homologous recombination at the targeted allele was demonstrated by Southern blot. Following confirmation of the disrupted allele, chimeric blastocysts were generated by micro injection into developing blastocysts derived from C57BL/6 female donors. Males from this litter displaying the highest degree of white coat color were bred to BALB/c females and progeny were genotyped by PCR to assess for germline transmission of the disrupted Il13ra1 allele. Only females having white coats and displaying the germ line transmission by PCR were bred back to a wild-type BALB/c male to generate Il13ra1tm1Zagh hemizygous males, which were than bred back with heterozygous females to generate Il13ra1tm1Zagh homozygous females. To confirm deletion of exons 7,8,9 in transcripts from the disrupted Il13ra1tm1Zagh allele, mRNA was extracted from intestinal epithelial cells of -/- and +/+ littermate controls, since intestinal epithelial cells express IL-13R alpha1 in non-manipulated mice. cDNA was synthesized from mRNA by reverse transcription PCR. The bands were purified and sequenced to confirm deletion of exons 7-9 in the Il13ra1tm1Zagh mice. Heterozygous females from the F1 generation were bred back to a SJL male, by use of speed congenics.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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