Strain Name:
B6;129-Syn2tm1Pggd Syn3tm1Pggd Syn1tm1Pggd/Mmjax
Stock Number:
041434-JAX
Citation ID:
RRID:MMRRC_041434-JAX
Other Names:
synapsin triple knockout (TKO)

Gene Information

Syn1tm1Pggd
Name: synapsin I; targeted mutation 1, Paul Greengard
Synonyms: SynI-, Syn1tm1Lli
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
Syn3tm1Pggd
Name: synapsin III; targeted mutation 1, Paul Greengard
Synonyms: synapsin III -, Syn3tm1Jfe
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Syn2tm1Pggd
Name: synapsin II; targeted mutation 1, Paul Greengard
Synonyms: Syn2tm1Kao
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Syn3
Name: synapsin III
Synonyms: Synapsin IIIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
NCBI: 27204
Homologene: 68320
Syn1
Name: synapsin I
Synonyms: Syn-1, Synapsin Ib, Synapsin Ia
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Knockout
NCBI: 20964
Homologene: 48483
Syn2
Name: synapsin II
Synonyms: 2900074L19Rik, Synapsin IIb, Synapsin IIa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
NCBI: 20965
Homologene: 49348
Genetic Alterations
Synapsin I, synapsin II and synapsin III are knocked out.
Genotype Determination
ES Cell Line
Not specified
Phenotype

Homozygous: Seizures.

Heterozygous: Not evaluated

Mammalian Phenotype Terms
Allelic Composition: Syn1tm1Lli/Syn1tm1Lli (Genetic Background: B6.129P2-Syn1tm1Lli )

Allelic Composition: Syn1tm1Lli/Syn1tm1Lli (Genetic Background: involves: 129/Sv )

Allelic Composition: Syn3tm1Jfe/Syn3tm1Jfe (Genetic Background: B6.129X1-Syn3tm1Jfe )

Allelic Composition: Syn3tm1Jfe/Syn3tm1Jfe (Genetic Background: involves: 129X1/SvJ )

Allelic Composition: Syn2tm1Kao/Syn2tm1Kao (Genetic Background: B6.Cg-Syn2tm1Kao )

Allelic Composition: Syn2tm1Kao/Syn2tm1Kao (Genetic Background: Not Specified )

MeSH Terms
  • Action Potentials/physiology
  • Animals
  • Brain/anatomy & histology
  • Brain/physiology
  • Brain/ultrastructure
  • Evoked Potentials/physiology
  • Hippocampus/physiology
  • Learning/physiology
  • Mice
  • Mice, Knockout
  • Multigene Family
  • Neural Inhibition/physiology
  • Neuronal Plasticity/physiology
  • Neurotransmitter Agents/metabolism
  • Phenotype
  • Synapses/classification
  • Synapses/physiology
  • Synapsins/genetics
  • Synapsins/physiology
  • Synaptic Transmission/physiology
  • Synaptic Vesicles/physiology
Strain Development
Mice have been inbred only to preserve the homozygosity of each of the three knockouts in the same mouse.
Suggested Control Mice
Wild-type littermates
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Paul Greengard, Ph.D., The Rockefeller University.
Primary Reference

Gitler D, Takagishi Y, Feng J, Ren Y, Rodriguiz RM, Wetsel WC, Greengard P, Augustine GJ. Different presynaptic roles of synapsins at excitatory and inhibitory synapses. J Neurosci. 2004 Dec 15;24(50):11368-80. (Medline PMID: 15601943)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Average litter size
4-6
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041434-JAX-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
041434-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.