Strain Name:
B6.129-Syn2tm1Pggd/Mmjax
Stock Number:
041437-JAX
Citation ID:
RRID:MMRRC_041437-JAX
Other Names:
Synapsin II Knockout, Syn2 KO

Strain Information

Syn2tm1Pggd
Name: synapsin II; targeted mutation 1, Paul Greengard
Synonyms: Syn2tm1Kao
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Syn2
Name: synapsin II
Synonyms: 2900074L19Rik, Synapsin IIb, Synapsin IIa
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 20965
Homologene: 49348
Genetic Alterations
Homozygous synapsin II (Syn2) exon 1 knockout mice exhibit seizures and retarded axon formation. These mice may be useful in studying the role of synapsin II in neurodevelopment.
Genotype Determination
ES Cell Line
Unspecified.
Phenotype
The Syn2 gene is a member of the synapsin family of neuronal phophsoproteins, which are associated with neurotransmitter release and synaptogenesis. Synapsin proteins are associated with autism. Synapsin II localizes to presynaptic terminals. The synapsin II knockout allele has a PGK-neo cassette replacing exon 1 of the Syn2 gene. Homozygous mice exhibit decreased brain weight, impaired lamellipodia formation, abnormal neurite formation, aberrant actin filament distribution, delayed axon differentiation, altered cytoskeletal formation, and to a lesser extent than the synapsin I KO, delayed synapse formation. Beginning at two months of age, mice experience spontaneous seizures. Homozygous mice are viable and fertile with no reported reproductive deficiency.
Mammalian Phenotype Terms
Allelic Composition: Syn2tm1Kao/Syn2tm1Kao (Genetic Background: B6.Cg-Syn2tm1Kao )

Allelic Composition: Syn2tm1Kao/Syn2tm1Kao (Genetic Background: Not Specified )

MeSH Terms
  • Animals
  • Axons/metabolism
  • Cells, Cultured
  • Hippocampus/growth & development
  • Hippocampus/metabolism
  • Immunohistochemistry
  • Mice
  • Mice, Knockout
  • Nervous System/growth & development
  • Neurons/metabolism
  • Synapses/metabolism
  • Synapsins/genetics
  • Synapsins/metabolism
  • Synapsins/physiology
Strain Development
A targeting vector was designed by Dr. Paul Greengard (Rockefeller University) to replace exon 1 of the synapsin II gene (Syn2) on chromosome 6 with a PGK-neomycin cassette. The construct was electroporated into 129-derived unspecified embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts, and chimeric males were bred with C57BL/6J females to establish the colony. The resulting mice were bred with C57BL/6 wildtype mice for at least ten generations prior to sending males to The Jackson Laboratory (MMRRC) in 2015.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Paul Greengard, Ph.D., The Rockefeller University.
Primary Reference

Ferreira A, Chin LS, Li L, Lanier LM, Kosik KS, Greengard P. Distinct roles of synapsin I and synapsin II during neuronal development. Mol Med. 1998 Jan;4(1):22-8. (Medline PMID: 9513186)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
  • Homozygous female x Homozygous male littermate
Generation
>10
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 to 8 months 6 to 8 months
Average litter size
5-9
Recommended wean age
4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041437-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
041437-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.