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Strain Detail Sheet

Strain Name:
C57BL/6J-Tg(Gt(ROSA)26Sor-Gak*)1Legr/Mmmh
Stock Number:
041467-MU
Citation ID:
RRID:MMRRC_041467-MU
Other Names:
GAK-C62

Strain Information

Gene Details

Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Gak
Name: cyclin G associated kinase
Synonyms: D130045N16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231580
HGNC: HGNC:4113
Homologene: 3846

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tg(Gt(ROSA)26Sor-Gak*)1Legr
Name: transgene insertion 1, Lois Greene
Synonyms: C62, GAK-C62
Type: Transgene
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Transgenic
Genetic Alterations
In addition to full-length GAK, this mouse expresses a C-terminal GAK fragment consisting of the clathrin-binding and the J-domains of GAK.
Genotype Determination

Strain Description

Phenotype

Homozygous: No phenotype provided that the endogenous GAK protein is expressed.

Hemizygous: No observed phenotype.

Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Cell Biology
  • Diabetes
  • Metabolism
  • Neurobiology
  • Obesity

Strain Origin

Donor
Lois Greene, Ph.D., National Heart, Lung, and Blood Institute (NHLBI)
Strain Development
The GAK-C62 fragment was expressed from the ROSA 26 promoter. The fragment was cloned into the AgeI and EcoRI restriction sites that are present in the multiple-cloning site of the pBroad3 vector. The vector was linearized using the PacI restriction enzyme and then purified by using gel extraction. The vector was then injected into fertilized C57BL/6 mouse eggs, followed by their implantation into pseudopregnant recipient mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Backcross
Breeding Scheme(s)
  • Backcross: confirm there are no reproduction issues
    • Wild-type (STRAIN) female x Heterozygous male or reciprocal mating
    • Wild-type (STRAIN) female x Hemizygous male or reciprocal mating
  • Sib-mating: confirm there are no reproduction issues
    • Wild-type female x Heterozygous male littermate or reciprocal mating
    • Wild-type female x Hemizygous male littermate or reciprocal mating
    • Heterozygous female x Heterozygous male littermate
    • Homozygous female x Homozygous male littermate
  • Intra-strain Random Mating
    • Wild-type female x Heterozygous male from the colony or reciprocal mating
    • Wild-type female x Hemizygous male from the colony or reciprocal mating
    • Heterozygous female x Heterozygous male from the colony
    • Homozygous female x Homozygous male from the colony
Generation
25
Overall Breeding Performance
Excellent

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: > 12 months > 12 months
Bred to Homozygosity
No
Average litter size
5-9
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.