Loading...

Strain Detail Sheet

Strain Name:
B6.129P2-Gpr68tm1Bhk/Mmnc
Stock Number:
041473-UNC
Citation ID:
RRID:MMRRC_041473-UNC
Other Names:
GPR68, OGR1

Strain Information

Gene Details

Gpr68tm1Bhk
Name: G protein-coupled receptor 68; targeted mutation 1, Beverly H Koller
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knockout
Gpr68
Name: G protein-coupled receptor 68
Synonyms: OGR1
Type: Gene
Species: Mouse
Chromosome: 12
Alteration at locus: Knockout
NCBI: 238377
HGNC: HGNC:4519
Homologene: 2603

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The targeting construct for the Gpr68 locus consists of a PGK-1 promoter-driven neomycin resistance cassette flanked by two arms of homology with the mouse Gpr68 locus. The longer arm of homology was generated using a 7,266-bp PstI fragment extending from the last intron to the beginning of the last exon. This exon contains the entire coding sequence of the Gpr68 gene. The 1,335-bp shorter arm was generated by PCR amplification and extends from the downstream end of the long arm into the 3′ untranslated region of the gene. Homologous recombination of the targeting construct with the Gpr68 locus inserts the neomycin resistance cassette into codon 78 of the gene, thereby disrupting expression.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    E14TG2a derived from 129P2/OlaHsd

    Strain Description

    Phenotype

    Homozygous: Mice homozygous for a null allele exhibit decreased osteoclastogenesis, abnormal pH-sensitive osteoclast survival, and background sensitive alterations in brown adipose tissue, monocytes, and macrophages. Additionally, Ogerin transiently and reversibly reduced contextual-based fear memory in wild-type but not Gpr68-knockout mice.

    Heterozygous: Not evaluated

    MeSH Terms
    • Allosteric Regulation/drug effects
    • Allosteric Site
    • Animals
    • Anti-Anxiety Agents/analysis
    • Anti-Anxiety Agents/chemistry
    • Anti-Anxiety Agents/metabolism
    • Anti-Anxiety Agents/pharmacology
    • Benzyl Alcohols/analysis
    • Benzyl Alcohols/chemistry
    • Benzyl Alcohols/metabolism
    • Benzyl Alcohols/pharmacology
    • Conditioning, Classical
    • Drug Discovery
    • Fear
    • Female
    • HEK293 Cells
    • Humans
    • Ligands
    • Lorazepam/analysis
    • Lorazepam/chemistry
    • Lorazepam/metabolism
    • Lorazepam/pharmacology
    • Male
    • Memory/drug effects
    • Mice
    • Mice, Knockout
    • Models, Molecular
    • Receptors, G-Protein-Coupled/agonists
    • Receptors, G-Protein-Coupled/antagonists & inhibitors
    • Receptors, G-Protein-Coupled/chemistry
    • Receptors, G-Protein-Coupled/deficiency
    • Receptors, G-Protein-Coupled/metabolism
    • Signal Transduction/drug effects
    • Triazines/analysis
    • Triazines/chemistry
    • Triazines/metabolism
    • Triazines/pharmacology
    Strain Development
    Chimaeras were bred to C57BL/6J mice and pups carrying the mutant allele identified. After ten successive crosses of heterozygous animals to C57BL/6J mice, heterozygous mice were intercrossed and a congenic Gpr68tm1Bhk  breeding colony was established.
    Suggested Control Mice
    Wild-type littermates

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Immunology and Inflammation
    • Models for Human Disease
    • Neurobiology

    Strain Origin

    Donor
    Beverly Koller, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Huang, XP, Karpiak J, Kroeze WK, Zhu H, Chen X, Moy SS, Saddoris KA, Nikolova VD, Farrell MS, Wang S, Mangano TJ, Deshpande DA, Jiang A, Penn RB, Jin J, Koller BH, Kenakin T, Shoichet BK, Roth BL. Allosteric ligands for the pharmocologically dark receptors GPR68 and GPR65. Nature. 2015 Nov 26;527(7579):477-83. Epub 2015 Nov 9 (Medline PMID:26550826)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    Wild-type female x Heterozygous male from the colony or reciprocal mating
    Heterozygous female x Heterozygous male from the colony
    Homozygous female x Homozygous male from the colony
    Generation
    N10
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: > 12 months > 12 months
    Average litter size
    10-14
    Recommended wean age
    3 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    041473-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    041473-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.