Strain Name:
B6.129P2-Pitx3tm1Mli/Mmjax
Stock Number:
041479-JAX
Citation ID:
RRID:MMRRC_041479-JAX
Other Names:
Pitx3-GFP

Gene Information

Pitx3tm1Mli
Name: paired-like homeodomain transcription factor 3; targeted mutation 1, Meng Li
Synonyms: Pitx3GFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Pitx3
Name: paired-like homeodomain transcription factor 3
Synonyms: Ptx3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
NCBI: 18742
HGNC: HGNC:9006
Homologene: 3689
Genetic Alterations
Green Fluorescent Protein (GFP) was inserted into the Pitx3 locus via homologous recombination.
Genotype Determination
ES Cell Line
E14TG2a derived from 129P2/OlaHsd
Phenotype

Homozygous: The most distinguishing phenotype of this mouse is that their eyes are closed due to the lack of lens development. There is a dearth of substantia nigra and ventral tegmental area cells.

Heterozygous: Heterozygous mice do not have their eyes closed. Also, in the brain, the Pitx3-directed GFP fluorescence is observed in the dopaminergic neurons in the substantia nigra and ventral tegmental area.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Cells, Cultured
  • Dopamine/biosynthesis
  • Dopamine/genetics
  • Embryo, Mammalian
  • Female
  • Gene Expression Regulation, Developmental/physiology
  • Green Fluorescent Proteins
  • Homeodomain Proteins/biosynthesis
  • Homeodomain Proteins/genetics
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Male
  • Mesencephalon/cytology
  • Mesencephalon/embryology
  • Mesencephalon/metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neurons/cytology
  • Neurons/metabolism
  • Pregnancy
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics
Strain Development

To integrate a Pitx3-directed enhanced GFP (eGFP) reporter as well as to generate a Pitx3-deficient locus, a DNA sequence including part of intron 1, coding exons 2,3, and half of exon 4, was replaced with an engrailed 2 splice acceptor-eGFP-ires-pac-pA cassette (SA-eGFP-ires-pacl, where pac is puromycin-resistant gene). For selection of ES cell transfectants following electroporation, a floxed phosphoglycerate kinase (PGK) promoter-driven hygromycin-thymidine kinase (PGK-hygro-tk) fusion gene was placed downstream of the eGFP reporter cassette.

The linearized targeting vector was electroporated into E14TG2a ES cells. Four homologous recombinants were obtained. One of the recombinant clones was then electroporated with a Cre-expressing vector followed by selection with gancyclovir. Following confirmation of deletion of the PGK-hygro-tk cassettes, ES cells were injected into blastocysts. The three best chimeric males were mated to C57BL/6 females.

Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
Donor
Meng Li, BMed, PhD, Cardiff School of Biosciences.
Primary Reference

Zhao S, Maxwell S, Jimenez-Beristain A et al. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons. Eur J Neurosci. 2004 Mar;19(5):1133-40. (Medline PMID: 15016072)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Breeding Scheme(s)
  • Wild-type female x Heterozygous male littermate or reciprocal mating
  • Heterozygous female x Heterozygous male littermate
  • Homozygous female x Homozygous male littermate
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months >12 months
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041479-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
041479-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.