Strain Name:
B6.129P2-Zcchc11Gt(RRR277)Byg/JpmzMmucd
Stock Number:
041492-UCD
Citation ID:
RRID:MMRRC_041492-UCD
Other Names:
Zcchc11, TUT4

Gene Information

Tut4Gt(RRR277)Byg
Name: terminal uridylyl transferase 4; gene trap RRR277, BayGenomics
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Gene Trap
Tut4
Name: terminal uridylyl transferase 4
Synonyms: Tent3a, 6030404K05Rik, Zcchc11, 9230115F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Gene Trap
NCBI: 230594
Homologene: 35279
Genetic Alterations
The gene-trap genomic insertion site was located within intron 4 of Zcchc11, generating a fusion protein containing the first 314 amino acids of Zcchc11 joined in frame with the β-galactosidase reporter. All known conserved protein motifs and domains in Zcchc11 are downstream of this site.
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype

Homozygous: Homozygous mutant mice appear smaller than wild-type counterparts and have fertility issues.

Heterozygous: No observed phenotype.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Cell Differentiation
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Embryonic Development/genetics
  • Gene Expression Regulation
  • High-Throughput Nucleotide Sequencing
  • Insulin-Like Growth Factor I/genetics
  • Insulin-Like Growth Factor I/metabolism
  • Liver/metabolism
  • Mice
  • MicroRNAs/genetics
  • MicroRNAs/metabolism
  • RNA, Messenger/metabolism
  • Uridine/genetics
  • Uridine/metabolism
Strain Development

C57BL/6 blastocysts were  microinjected with mutant ES cells from cell line RRR277 containing a gene-trap insertion in the Zcchc11 gene to create chimeric mice. Mice were subsequently backcrossed onto a C57BL/6 genetic background  for at least 10 generations.

Suggested Control Mice
Wild-type littermates
Donor
Joseph Mizgerd, Ph.D., Harvard University.
Primary Reference

Jones MR, Blahna MT, Kozlowski E, Matsuura KY, Ferrari JD, Morris SA, Powers JT, Daley GQ, Quinton LJ, Mizgerd JP. Zcchc11 uridylates mature miRNAs to enhance neonatal IGF-1 expression, growth, and survival. PLoS Genet. 2012;8(11)(Medline PMID: 23209448)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Backcross
Breeding Scheme(s)

Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating

Generation
N10
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041492-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
041492-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
041492-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.