Strain Name:
STOCK Tbx5tm1.1Flc Gt(ROSA)26Sortm1.1(BirA)Mejr/Mmnc
Stock Number:
041509-UNC
Citation ID:
RRID:MMRRC_041509-UNC
Other Names:
Tbx5Avi/Avi;Rosa26BirA/BirA, Tbx5Avi;BirA

Strain Information

Gt(ROSA)26Sortm1.1(birA)Mejr
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
Synonyms: bira, Rosa26BirA
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: ROSA26, beta geo, Gtrgeo26, Gtrosa26, R26, Thumpd3as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
NCBI: 14910
Tbx5tm1.1Flc
Name: T-box 5; targeted mutation 1.1 Frank Conlon
Synonyms: Tbx5Avi
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knock-In
Tbx5
Name: T-box 5
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knock-In
NCBI: 21388
Homologene: 160
Genetic Alterations
The endogenous Tbx5 locus has a biotin acceptor peptide (Avi-tag) knocked-in in-frame to the Tbx5 locus. This mouse is then crossed to a Rosa26BirA/BirA mouse to have endogenous biotinylated Tbx5.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Not specified
    Phenotype
    • Homozygous: No phenotype. Normal fertility.
    • Heterozygous: No phenotype. Normal fertility.
    MeSH Terms
    • Animals
    • Chromatin Assembly and Disassembly/genetics
    • Gene Expression Regulation, Developmental/genetics
    • Heart/embryology
    • Humans
    • Mice, Transgenic
    • Myocardium/metabolism
    • Organogenesis/genetics
    • Organogenesis/physiology
    • T-Box Domain Proteins/genetics
    • T-Box Domain Proteins/metabolism
    • Transcription, Genetic/genetics
    Strain Development
    Mouse embryonic stem cells containing the Tbx5Avi-Neo allele were injected into C57BL/6 mouse blastocysts. Chimeric males were mated to C57BL/6 females. Offspring were mated to Sox2-Cre mice (B6N.Cg-Tg(Sox2-Cre)1AMC/J, JAX Strain #014094) to remove the Neo cassette. Tbx5tm1.1Flc progeny were mated to mice containing Gt(ROSA)26Sortm1.1(BirA)Mejr and brought to double homozygosity.
    Suggested Control Mice
    Wild-type littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Developmental Biology
    • Internal Organ
    • Models for Human Disease
    • Research Tools
    Donor
    Frank Conlon, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Waldron L, Steimle JD, Greco TM, Gomez NC, Dorr KM, Kweon J, Temple B, YangXH, Wilczewski CM, Davis IJ, Cristea IM, Moskowitz IP, Conlon FL. The CardiacTBX5 Interactome Reveals a Chromatin Remodeling Network Essential for CardiacSeptation. Dev Cell. 2016 Feb 8;36(3):262-75. doi: 10.1016/j.devcel.2016.01.009. (Medline PMID: 26859351)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    black or brown
    MMRRC Breeding System
    Sib-mating or Intra-strain Random Mating
    Breeding Scheme(s)
    • Sib-mating:
      • Wild-type female x Heterozygous male littermate or reciprocal mating
      • Wild-type female x Hemizygous male littermate or reciprocal mating
      • Heterozygous female x Heterozygous male littermate
      • Homozygous female x Homozygous male littermate
    • Intra-strain Random Mating
      • Wild-type female x Heterozygous male from the colony or reciprocal mating
      • Wild-type female x Hemizygous male from the colony or reciprocal mating
      • Heterozygous female x Heterozygous male from the colony
      • Homozygous female x Homozygous male from the colony
    Generation
    N2F7
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: >12 months >12 months
    Average litter size
    10-14
    Recommended wean age
    3-4 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    041509-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    041509-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.