Strain Name:
B6.129X1-Id4tm1Mais/Mmjax
Stock Number:
041569-JAX
Citation ID:
RRID:MMRRC_041569-JAX
Other Names:
Id4 null, Id4-/-, Id4 knockout, Id4 mutant

Gene Information

Id4tm1Mais
Name: inhibitor of DNA binding 4; targeted mutation 1, Mark A Israel
Synonyms: Id4GFP, Id4-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Id4
Name: inhibitor of DNA binding 4
Synonyms: Id4, Idb4, bHLHb27
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
NCBI: 15904
VEGA: 13
HGNC: HGNC:5363
Homologene: 1186
Genetic Alterations
A replacement vector was designed that deletes two exons that include the coding region of mouse ID4 protein.
Genotype Determination
ES Cell Line
JM-1 derived from 129X1/SvJ
Phenotype

Homozygous: abnormal white adipose tissue physiology, abnormal brown adipose tissue physiology, abnormal nervous system development

Heterozygous: None obvious or not characterized.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Brain/abnormalities
  • Brain/cytology
  • Brain/embryology
  • Brain/metabolism
  • Cell Differentiation
  • Cell Proliferation
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • G1 Phase
  • Inhibitor of Differentiation Proteins
  • Mice
  • Mice, Knockout
  • Mutation/genetics
  • Neurons/cytology
  • Neurons/metabolism
  • S Phase
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Time Factors
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • ARNTL Transcription Factors
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Biological Clocks/physiology
  • CLOCK Proteins
  • Circadian Rhythm/physiology
  • Gene Expression Regulation
  • Inhibitor of Differentiation Protein 2/genetics
  • Inhibitor of Differentiation Protein 2/metabolism
  • Inhibitor of Differentiation Proteins/genetics
  • Inhibitor of Differentiation Proteins/metabolism
  • Motor Activity/physiology
  • Myocardium/metabolism
  • Photoperiod
  • Promoter Regions, Genetic
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Suprachiasmatic Nucleus/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • 3T3-L1 Cells
  • Adipocytes/cytology
  • Adipose Tissue/metabolism
  • Body Weight
  • Dimerization
  • Fibroblasts/metabolism
  • Gene Deletion
  • Genotype
  • Homozygote
  • Inhibitor of Differentiation Proteins/physiology
  • Mice, Inbred C57BL
  • Models, Biological
Strain Development
Id4-deficient mice were initially established on a C57BL/6J background and maintained on that background thereafter. Mice were developed prior to 2003 at Dartmouth Medical School. Current colony transported to the University of Notre Dame in 2006.
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Developmental Biology
  • Internal Organ
  • Neurobiology
  • Obesity
Donor
Giles Duffield, Ph.D., University of Notre Dame.
Primary Reference

Yun K, Mantani A, Garel S, Rubenstein J, Israel MA. Id4 regulates neural progenitor proliferation and differentiation in vivo. Development. 2004 Nov;131(21):5441-8. Epub 2004 Oct 6. (Medline PMID: 15469968)

Duffield GE, Watson NP, Mantani A, Peirson SN, Robles-Murguia M, Loros JJ, Israel MA, Dunlap JC. A role for Id2 in regulating photic entrainment of the mammalian circadian system. Curr Biol. 2009 Feb 24;19(4):297-304. Epub 2009 Feb 12. (Medline PMID: 19217292)

Murad JM, Place CS, Ran C, Hekmatyar SK, Watson NP, Kauppinen RA, Israel MA. Inhibitor of DNA binding 4 (ID4) regulation of adipocyte differentiation and adipose tissue formation in mice. J Biol Chem. 2010 Jul 30;285(31):24164-73. Epub 2010 May 11. (Medline PMID: 20460371)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
black
MMRRC Breeding System
Random intra-strain mating
Generation
none
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041569-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
041569-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.