Strain Name:
B6.129X1-Id4tm1Mais/Mmjax
Stock Number:
041569-JAX
Citation ID:
RRID:MMRRC_041569-JAX
Other Names:
Id4 null, Id4-/-, Id4 knockout, Id4 mutant

Strain Information

Id4tm1Mais
Name: inhibitor of DNA binding 4; targeted mutation 1, Mark A Israel
Synonyms: Id4-, Id4GFP
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Id4
Name: inhibitor of DNA binding 4
Synonyms: Id4, Idb4, bHLHb27
Type: Gene
Species: Mouse
Chromosome: 13
Alteration at locus: Knockout
NCBI: 15904
VEGA: 13
HGNC: HGNC:5363
Homologene: 1186
Genetic Alterations
A replacement vector was designed that deletes two exons that include the coding region of mouse ID4 protein. Id4 knock-in/knock-out mice exhibit reduced brain size, impaired cell cycle transition in early neural stem cells, reduced body fat, resistance to high fat diet and impaired adipocyte differentiation. This strain may be useful for studying ID4 as a regulator of neurogenesis and adipogenesis.
ES Cell Line
JM-1
Phenotype
ID4 (inhibitor of DNA binding 4) is a transcriptional regulator containing a helix-loop-helix domain and lacking a DNA binding domain. Unique among the ID proteins, ID4 is involved in the regulation of neural stem cell differentiation and proliferation. The Id4 knockout/knockin allele has a GFP reporter and a PGK-neo cassette replacing exons 1-2 of the gene. Uniform GFP expression is observed in all cortical areas by E12.5. Mice homozygous for this null allele exhibit reduced brain size from E11.5, specifically a 30% reduction occurs in the ventricular surface of the telencephalon affecting dorsomedial progenitor zones. Impairment of G1-S cell cycle transition and premature differentiation in early neural stem cells results in reduced brain size in these mice. Like ID2, loss of ID4 affects adipogenesis. Homozygous mice have 50% less body fat weight and adipocyte size is reduced by half. Following 6 weeks on high fat diet, mice gain significantly less weight than wild-type littermates. This strain may be useful for studying ID4 as a regulator of neurogenesis and adipogenesis.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Brain/abnormalities
  • Brain/cytology
  • Brain/embryology
  • Brain/metabolism
  • Cell Differentiation
  • Cell Proliferation
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • G1 Phase
  • Inhibitor of Differentiation Proteins
  • Mice
  • Mice, Knockout
  • Mutation/genetics
  • Neurons/cytology
  • Neurons/metabolism
  • S Phase
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Time Factors
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • ARNTL Transcription Factors
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Biological Clocks/physiology
  • CLOCK Proteins
  • Circadian Rhythm/physiology
  • Gene Expression Regulation
  • Inhibitor of Differentiation Protein 2/genetics
  • Inhibitor of Differentiation Protein 2/metabolism
  • Inhibitor of Differentiation Proteins/genetics
  • Inhibitor of Differentiation Proteins/metabolism
  • Motor Activity/physiology
  • Myocardium/metabolism
  • Photoperiod
  • Promoter Regions, Genetic
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism
  • Suprachiasmatic Nucleus/metabolism
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • 3T3-L1 Cells
  • Adipocytes/cytology
  • Adipose Tissue/metabolism
  • Body Weight
  • Dimerization
  • Fibroblasts/metabolism
  • Gene Deletion
  • Genotype
  • Homozygote
  • Inhibitor of Differentiation Proteins/physiology
  • Mice, Inbred C57BL
  • Models, Biological
Strain Development
A targeting vector containing the GFP (green fluorescent protein) coding regions and the neomycin resistance cassette was used to replace exons 1 and 2 through homologous recombination. The construct was electroporated into electroporated into 129X1/SvJ-derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice and then backcrossed to C57BL/6J in excess of 5 generations. Upon arrival at The Jackson Laboratory MMRRC, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Cancer
  • Developmental Biology
  • Internal Organ
  • Neurobiology
  • Obesity
Donor
Giles Duffield, Ph.D., University of Notre Dame.
Primary Reference

Yun K, Mantani A, Garel S, Rubenstein J, Israel MA. Id4 regulates neural progenitor proliferation and differentiation in vivo. Development. 2004 Nov;131(21):5441-8. Epub 2004 Oct 6. (Medline PMID: 15469968)

Duffield GE, Watson NP, Mantani A, Peirson SN, Robles-Murguia M, Loros JJ, Israel MA, Dunlap JC. A role for Id2 in regulating photic entrainment of the mammalian circadian system. Curr Biol. 2009 Feb 24;19(4):297-304. Epub 2009 Feb 12. (Medline PMID: 19217292)

Murad JM, Place CS, Ran C, Hekmatyar SK, Watson NP, Kauppinen RA, Israel MA. Inhibitor of DNA binding 4 (ID4) regulation of adipocyte differentiation and adipose tissue formation in mice. J Biol Chem. 2010 Jul 30;285(31):24164-73. Epub 2010 May 11. (Medline PMID: 20460371)

Colony and Husbandry Information

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.
Coat Color
black
MMRRC Breeding System
Random intra-strain mating
Generation
none
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041569-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
041569-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.