Strain Name:
B6.129S7(FVB)-Dbhem2.1(flpo)Rray/Mmucd
Stock Number:
041575-UCD
Citation ID:
RRID:MMRRC_041575-UCD
Other Names:
DBH p2a FlpO

Gene Information

Allele Symbol: Dbhem2.1(flpo)Rray (Mus musculus (mouse))
Name: dopamine beta hydroxylase; endonuclease-mediated mutation 2.1, Russell Ray
Synonyms: Dbh, DBH p2a FlpO, Dbh, DBH-p2a-FLPo
Chromosome: 2
Alteration at locus: CRISPR
Monarch Initiative (Disease and Phenotype Associations): MGI:5770774
Gene Symbol: Dbh (Mus musculus (mouse))
Name: dopamine beta hydroxylase
Chromosome: 2
Alteration at locus: CRISPR
Monarch Initiative (Disease and Phenotype Associations): MGI:94864
Genetic Alterations

The p2a-FLPo recombinase sequence was knocked into the stop codon of the dopamine-beta-hydroxylase (Dbh)gene to preserve gene function using AB2.2 ES cells.

ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
Phenotype

Homozygous: No observed phenotype

Hemizygous: No observed phenotype

MeSH Terms
  • Animals
  • Brain Stem/metabolism
  • CRISPR-Cas Systems/genetics
  • Catecholamines/metabolism
  • DNA Nucleotidyltransferases/metabolism
  • Dopamine beta-Hydroxylase/genetics
  • Embryonic Stem Cells/metabolism
  • Gene Knock-In Techniques/methods
  • Gene Targeting/methods
  • Heterozygote
  • Homozygote
  • Inbreeding
  • Mice
  • Mice, Transgenic
  • RNA, Guide/metabolism
Strain Development
Correctly targeted AB2.2 ES cells were injected into C57BL/6J blastocysts, and chimeras were bred to C57BL/6J. Offspring were bred to B-actin Cre to remove the neo cassete, after which mice were crossed to C57BL/6J for 3 generations. 
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
  • Sensorineural
Donor
Russell Ray, Ph.D., Baylor College of Medicine.
Primary Reference

Sun JJ, Ray R. Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016 Jul 21. (Medline PMID: 27441631)

Colony and Husbandry Informationon

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Backcross
Generation
3
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041575-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
041575-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
041575-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.