Strain Name:
Stock Number:
Citation ID:
Other Names:
DBH p2a FlpO

Strain Information

Name: dopamine beta hydroxylase; endonuclease-mediated mutation 2.1, Russell Ray
Synonyms: Dbhtm2(DBH-FLPo)Rray, DBH p2a FlpO, DBH-p2a-FLPo, Dbhem2.1(flpo)Rray
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
Name: dopamine beta hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: CRISPR
NCBI: 13166
Homologene: 615
Genetic Alterations

The p2a-FLPo recombinase sequence was knocked into the stop codon of the dopamine-beta-hydroxylase (Dbh) gene to preserve gene function using AB2.2 ES cells.

ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2

Homozygous: No observed phenotype

Hemizygous: No observed phenotype

MeSH Terms
  • Animals
  • Brain Stem/metabolism
  • CRISPR-Cas Systems/genetics
  • Catecholamines/metabolism
  • DNA Nucleotidyltransferases/metabolism
  • Dopamine beta-Hydroxylase/genetics
  • Embryonic Stem Cells/metabolism
  • Gene Knock-In Techniques/methods
  • Gene Targeting/methods
  • Heterozygote
  • Homozygote
  • Inbreeding
  • Mice
  • Mice, Transgenic
  • RNA, Guide/metabolism
Strain Development

Correctly targeted AB2.2 ES cells were injected into C57BL/6J blastocysts, and chimeras were bred to C57BL/6J. Offspring were bred to B-actin Cre (B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ - RRID:IMSR_JAX:019099) to remove the neo cassette, after which mice were crossed to C57BL/6J for 3 generations.

Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
  • Sensorineural
Russell Ray, Ph.D., Baylor College of Medicine.
Primary Reference

Sun JJ, Ray R. Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016 Jul 21. (Medline PMID: 27441631)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color
MMRRC Breeding System
N5 (includes both, C57BL/6J and intermittent C57BL/6N with Cre deleter)
Overall Breeding Performance
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Average litter size
Recommended wean age
3 weeks
Average Pups Weaned

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041575-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
041575-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
041575-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.