Strain Name:
B6.129S7(FVB)-Dbhem1.1(flpo)Rray/Mmucd
Stock Number:
041577-UCD
Citation ID:
RRID:MMRRC_041577-UCD
Other Names:
DBH-FLPo

Gene Information

Dbhem1.1(flpo)Rray
Name: dopamine beta hydroxylase; endonuclease-mediated mutation 1.1, Russell Ray
Synonyms: Dbhtm1(DBH-FLPo)Rray, DBH-FLPo
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Endonuclease-mediated
Dbh
Name: dopamine beta hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Endonuclease-mediated
NCBI: 13166
HGNC: HGNC:2689
Homologene: 615
flpo
Name: mouse codon-optimized FLP recombinase, FLPo
Type: Gene
Species: Mus musculus (mouse)
Alteration at locus: Endonuclease-mediated
Genetic Alterations
FLPo recombinase (PMID:17225864) was knocked into the Dbh start codon, with deletion of 106 bp in the first exon.
Genotype Determination
ES Cell Line
AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2
Phenotype

Homozygous: Embryonically lethal

Hemizygous: No observed phenotype

MeSH Terms
  • Animals
  • Brain Stem/metabolism
  • CRISPR-Cas Systems/genetics
  • Catecholamines/metabolism
  • DNA Nucleotidyltransferases/metabolism
  • Dopamine beta-Hydroxylase/genetics
  • Embryonic Stem Cells/metabolism
  • Gene Knock-In Techniques/methods
  • Gene Targeting/methods
  • Heterozygote
  • Homozygote
  • Inbreeding
  • Mice
  • Mice, Transgenic
  • RNA, Guide/metabolism
  • DNA Nucleotidyltransferases/genetics
  • Embryo, Mammalian/anatomy & histology
  • Embryo, Mammalian/physiology
  • Genes, Reporter
  • Genetic Engineering
  • Integrases/genetics
  • Integrases/metabolism
  • Recombination, Genetic
Strain Development
Made in AB2.2 derived from 129S7/SvEvBrd-Hprtb-m2 ES cells, then injected into C57BL/6J. Mice were crossed to B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ (JAX Strain #019099, N14+ to C57BL/6N), then bred 3 generations to C57BL/6J, hence incipient congenic.
Suggested Control Mice
Wild-type littermates
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Diabetes
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
  • Sensorineural
Donor
Russell Ray, Ph.D., Baylor College of Medicine.
Primary Reference

Sun JJ, Ray R. Generation of Two Noradrenergic-Specific Dopamine-Beta-Hydroxylase-FLPo Knock-In Mice Using CRISPR/Cas9-Mediated Targeting in Embryonic Stem Cells. PLoS One. 2016 Jul 21.(Medline PMID: 27441631)

Raymond CS, Soriano P. High-efficiency FLP and PhiC31 site-specificrecombination in mammalian cells. PLoS One. 2007 Jan 17;2(1):e162. (Medline PMID: 17225864)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
N5 (C57BL/6J, B6N-congenic Cre deleter at N14+, and three additional crosses to C57BL/6N)
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
5-9
Recommended wean age
3 weeks
Average Pups Weaned
5-9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041577-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
041577-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity2
041577-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.