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Strain Name:
C57BL/6J-MtgxR4514Btlr/Mmmh
Stock Number:
041588-MU
Citation ID:
RRID:MMRRC_041588-MU
Other Names:
R4514 (G1), C57BL/6J-MtgxR4514Btlr
Major Collection:

Strain Information

Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: RNCAM, R4B12 antigen, Ncam-2, Ocam
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, Tarpp, D9Bwg1012e, 0710001E13Rik, R3hdm3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74100
Homologene: 32306
Mapk14
Name: mitogen-activated protein kinase 14
Synonyms: p38-alpha, p38, p38 MAP Kinase, Mxi2, CSBP2, Crk1, Csbp1, p38MAPK, p38a, p38 alpha, p38alpha, p38 MAP kinase alpha, p38 MAPK
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26416
HGNC: HGNC:6876
Homologene: 31777
Ppp1ca
Name: protein phosphatase 1 catalytic subunit alpha
Synonyms: Ppp1c, dism2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19045
VEGA: 19
HGNC: HGNC:9281
Homologene: 105262
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214812
Homologene: 72220
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: Nil2, [delta]EF1, MEB1, Tcf8, Zfx1a, Tcf18, AREB6, ZEB, Zfhep, 3110032K11Rik, Zfhx1a, Tw
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 21417
Homologene: 31779
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 93,322,133 bp
  • T to C, chromosome 1 at 128,300,514 bp
  • T to A, chromosome 1 at 164,151,997 bp
  • T to C, chromosome 2 at 12,182,736 bp
  • T to C, chromosome 2 at 88,487,365 bp
  • T to A, chromosome 2 at 116,880,314 bp
  • T to C, chromosome 2 at 127,748,087 bp
  • T to C, chromosome 2 at 158,204,802 bp
  • A to G, chromosome 3 at 19,988,013 bp
  • C to G, chromosome 4 at 51,229,651 bp
  • T to A, chromosome 4 at 126,039,860 bp
  • G to A, chromosome 4 at 147,583,563 bp
  • C to T, chromosome 5 at 65,164,690 bp
  • A to G, chromosome 5 at 76,230,199 bp
  • A to G, chromosome 5 at 87,930,138 bp
  • G to T, chromosome 5 at 105,479,953 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • T to C, chromosome 5 at 136,131,548 bp
  • A to G, chromosome 5 at 139,546,767 bp
  • T to C, chromosome 6 at 31,433,158 bp
  • A to G, chromosome 6 at 67,861,514 bp
  • G to A, chromosome 6 at 85,817,423 bp
  • A to G, chromosome 6 at 111,358,304 bp
  • T to A, chromosome 7 at 18,529,608 bp
  • T to C, chromosome 7 at 28,742,284 bp
  • C to G, chromosome 7 at 31,767,052 bp
  • C to T, chromosome 7 at 104,189,391 bp
  • A to G, chromosome 7 at 139,910,286 bp
  • G to A, chromosome 7 at 139,996,071 bp
  • A to T, chromosome 8 at 19,126,506 bp
  • T to G, chromosome 8 at 24,818,136 bp
  • C to T, chromosome 8 at 27,198,485 bp
  • A to G, chromosome 8 at 116,933,396 bp
  • G to A, chromosome 8 at 125,761,557 bp
  • C to A, chromosome 9 at 44,973,584 bp
  • C to T, chromosome 9 at 50,805,354 bp
  • A to G, chromosome 9 at 65,703,695 bp
  • T to A, chromosome 9 at 112,177,677 bp
  • T to C, chromosome 9 at 114,095,924 bp
  • T to C, chromosome 10 at 80,126,377 bp
  • T to C, chromosome 10 at 109,694,082 bp
  • T to G, chromosome 10 at 125,233,439 bp
  • G to A, chromosome 11 at 107,077,692 bp
  • A to G, chromosome 12 at 66,716,722 bp
  • T to A, chromosome 12 at 110,657,139 bp
  • T to C, chromosome 12 at 113,685,596 bp
  • C to G, chromosome 14 at 54,735,790 bp
  • A to G, chromosome 15 at 76,297,955 bp
  • A to T, chromosome 16 at 20,707,825 bp
  • A to T, chromosome 16 at 38,549,416 bp
  • T to A, chromosome 16 at 81,512,996 bp
  • T to C, chromosome 17 at 28,724,824 bp
  • A to T, chromosome 17 at 55,483,017 bp
  • G to A, chromosome 18 at 5,759,007 bp
  • T to A, chromosome 18 at 24,683,212 bp
  • T to G, chromosome 19 at 4,195,055 bp
  • A to T, chromosome 19 at 34,648,513 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041588-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.