Strain Name:
C57BL/6J-MtgxR4514Btlr/Mmmh
Stock Number:
041588-MU
Citation ID:
RRID:MMRRC_041588-MU
Other Names:
R4514 (G1), C57BL/6J-MtgxR4514Btlr
Major Collection:

Gene Information

Itga8
Name: integrin alpha 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Ncam2
Name: neural cell adhesion molecule 2
Synonyms: Ncam-2, Ocam, R4B12 antigen, RNCAM
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 17968
HGNC: HGNC:7657
Homologene: 3336
Arpp21
Name: cyclic AMP-regulated phosphoprotein, 21
Synonyms: ARPP-21, Tarpp, R3hdm3, D9Bwg1012e, 0710001E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74100
Homologene: 32306
Mapk14
Name: mitogen-activated protein kinase 14
Synonyms: Csbp1, Mxi2, CSBP2, Crk1, p38MAPK, p38, p38-alpha, p38a, p38 alpha, p38 MAPK, p38 MAP Kinase, p38 MAP kinase alpha, p38alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 26416
HGNC: HGNC:6876
Homologene: 31777
Ppp1ca
Name: protein phosphatase 1 catalytic subunit alpha
Synonyms: dism2, Ppp1c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19045
VEGA: 19
HGNC: HGNC:9281
Homologene: 105262
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214812
Homologene: 72220
Zeb1
Name: zinc finger E-box binding homeobox 1
Synonyms: AREB6, MEB1, [delta]EF1, Tcf8, ZEB, Zfhep, Zfx1a, Nil2, Tcf18, Zfhx1a, Tw, 3110032K11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 21417
Homologene: 31779
Bptf
Name: bromodomain PHD finger transcription factor
Synonyms: Falz, 9430093H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 207165
HGNC: HGNC:3581
Homologene: 114397
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: Loa, 9930018I23Rik, MAP1C, Dnec1, Swl, Dnchc1, dynein heavy chain, retrograde transport
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Zfp985
Name: zinc finger protein 985
Synonyms: Gm13154
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 433804
Homologene: 133076
Clock
Name: circadian locomotor output cycles kaput
Synonyms: bHLHe8, KAT13D, 5330400M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12753
HGNC: HGNC:2082
Homologene: 3603
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: SMN2, 6330570A01Rik, Tg(SMN2)89Ahmb, E130018M02Rik, mGluR7, mGlu7a receptor, Gpr1g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Stk11
Name: serine/threonine kinase 11
Synonyms: Lkb1, Par-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20869
Homologene: 393
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Tubgcp2
Name: tubulin, gamma complex associated protein 2
Synonyms: 1700022B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74237
Homologene: 55980
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: PHT1, PTR4, C130069N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100561
Homologene: 26432
Pask
Name: PAS domain containing serine/threonine kinase
Synonyms: Paskin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269224
Homologene: 9038
Uncx
Name: UNC homeobox
Synonyms: Chx4, Uncx4.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 22255
Homologene: 56598
Lct
Name: lactase
Synonyms: LOC226413, Lphl, LPH
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226413
HGNC: HGNC:6530
Homologene: 124204
Cp
Name: ceruloplasmin
Synonyms: D3Ertd555e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12870
HGNC: HGNC:2295
Homologene: 75
Slc7a8
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 8
Synonyms: LAT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50934
VEGA: 14
Homologene: 8166
Tmco5
Name: transmembrane and coiled-coil domains 5
Synonyms: 1700095F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67356
Homologene: 41661
Mocos
Name: molybdenum cofactor sulfurase
Synonyms: 1110018O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 68591
VEGA: 18
Homologene: 9931
F5
Name: coagulation factor V
Synonyms: Cf5, Cf-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Clcn2
Name: chloride channel, voltage-sensitive 2
Synonyms: Clc2, ClC-2, nmf240
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12724
HGNC: HGNC:2020
Homologene: 3213
Nav3
Name: neuron navigator 3
Synonyms: 9630020C08Rik, 4732483H20Rik, Pomfil1p, POMFIL1, unc53H3, steerin 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 260315
Homologene: 56688
Csf3r
Name: colony stimulating factor 3 receptor (granulocyte)
Synonyms: Cd114, G-CSFR, Csfgr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12986
HGNC: HGNC:2439
Homologene: 601
Got1l1
Name: glutamic-oxaloacetic transaminase 1-like 1
Synonyms: 1700083M11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76615
Homologene: 65045
Oplah
Name: 5-oxoprolinase (ATP-hydrolysing)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 75475
HGNC: HGNC:8149
Homologene: 90938
Nat8f5
Name: N-acetyltransferase 8 (GCN5-related) family member 5
Synonyms: 1810018F03Rik, Cml5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69049
Homologene: 87019
Slc16a7
Name: solute carrier family 16 (monocarboxylic acid transporters), member 7
Synonyms: 4921534N07Rik, D630004K10Rik, 9030411M13Rik, MCT2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20503
Homologene: 20990
Klhl5
Name: kelch-like 5
Synonyms: 1300013C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71778
HGNC: HGNC:6356
Homologene: 56736
Adam5
Name: a disintegrin and metallopeptidase domain 5
Synonyms: tMDCII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11499
HGNC: HGNC:212
Homologene: 49138
Mdga2
Name: MAM domain containing glycosylphosphatidylinositol anchor 2
Synonyms: Mamdc1, Mdga2, 9330209L04Rik, Adp, 6720489L24Rik, Tg(Prnp-PFN1*G118V)838Kiaei
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320772
Homologene: 45659
Pcnx2
Name: pecanex homolog 2
Synonyms: E330039K12Rik, Pcnxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270109
HGNC: HGNC:8736
Homologene: 8987
D630003M21Rik
Name: RIKEN cDNA D630003M21 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228846
Homologene: 18598
Susd5
Name: sushi domain containing 5
Synonyms: LOC382111
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382111
VEGA: 9
Homologene: 19526
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Olfr649
Name: olfactory receptor 649
Synonyms: GA_x6K02T2PBJ9-6924585-6923647, MOR31-7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259057
Homologene: 74119
Kndc1
Name: kinase non-catalytic C-lobe domain (KIND) containing 1
Synonyms: VKIND, B830014K08Rik, 2410012C07Rik, very-kind
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76484
Homologene: 45138
St6gal2
Name: beta galactoside alpha 2,6 sialyltransferase 2
Synonyms: C230064G14Rik, ST6Gal II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 240119
Homologene: 13052
Lrwd1
Name: leucine-rich repeats and WD repeat domain containing 1
Synonyms: 1200011O22Rik, Orca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71735
Homologene: 17678
Csn3
Name: casein kappa
Synonyms: CSN10, Csnk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12994
HGNC: HGNC:2446
Homologene: 3818
Defb34
Name: defensin beta 34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 360211
Poglut1
Name: protein O-glucosyltransferase 1
Synonyms: Rumi, Ktelc1, 9630046K23Rik, wsnp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224143
Homologene: 41353
Lrrc8b
Name: leucine rich repeat containing 8 family, member B
Synonyms: R75581, 2210408K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 433926
Homologene: 9103
Ifit1
Name: interferon-induced protein with tetratricopeptide repeats 1
Synonyms: Ifi56, ISG56
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 15957
Homologene: 22462
Psg25
Name: pregnancy-specific glycoprotein 25
Synonyms: cea13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114868
Homologene: 110989
Cenpn
Name: centromere protein N
Synonyms: 2610510J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 72155
Homologene: 12450
Alg9
Name: asparagine-linked glycosylation 9 (alpha 1,2 mannosyltransferase)
Synonyms: 8230402H15Rik, B430313H07Rik, Dibd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102580
Homologene: 6756
Nphp1
Name: nephronophthisis 1 (juvenile) homolog (human)
Synonyms: nephrocystin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 53885
HGNC: HGNC:7905
Homologene: 229
Cd3g
Name: CD3 antigen, gamma polypeptide
Synonyms: Ctg-3, Ctg3, T3g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12502
HGNC: HGNC:1675
Homologene: 55
Olfr1184
Name: olfactory receptor 1184
Synonyms: GA_x6K02T2Q125-49974190-49975125, MOR225-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258820
Homologene: 73980
Igkv17-127
Name: immunoglobulin kappa variable 17-127
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243433
Mrps12
Name: mitochondrial ribosomal protein S12
Synonyms: mitoribosomal protein s12, Rpms12, rps12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 24030
Homologene: 6848
Gm29627
Name: predicted gene 29627
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102635284
Homologene: 141213
Ighv2-5
Name: immunoglobulin heavy variable 2-5
Synonyms: Gm16593
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 638605
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 93,322,133 bp
  • T to C, chromosome 1 at 128,300,514 bp
  • T to A, chromosome 1 at 164,151,997 bp
  • T to C, chromosome 2 at 12,182,736 bp
  • T to C, chromosome 2 at 88,487,365 bp
  • T to A, chromosome 2 at 116,880,314 bp
  • T to C, chromosome 2 at 127,748,087 bp
  • T to C, chromosome 2 at 158,204,802 bp
  • A to G, chromosome 3 at 19,988,013 bp
  • C to G, chromosome 4 at 51,229,651 bp
  • T to A, chromosome 4 at 126,039,860 bp
  • G to A, chromosome 4 at 147,583,563 bp
  • C to T, chromosome 5 at 65,164,690 bp
  • A to G, chromosome 5 at 76,230,199 bp
  • A to G, chromosome 5 at 87,930,138 bp
  • G to T, chromosome 5 at 105,479,953 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • T to C, chromosome 5 at 136,131,548 bp
  • A to G, chromosome 5 at 139,546,767 bp
  • T to C, chromosome 6 at 31,433,158 bp
  • A to G, chromosome 6 at 67,861,514 bp
  • G to A, chromosome 6 at 85,817,423 bp
  • A to G, chromosome 6 at 111,358,304 bp
  • T to A, chromosome 7 at 18,529,608 bp
  • T to C, chromosome 7 at 28,742,284 bp
  • C to G, chromosome 7 at 31,767,052 bp
  • C to T, chromosome 7 at 104,189,391 bp
  • A to G, chromosome 7 at 139,910,286 bp
  • G to A, chromosome 7 at 139,996,071 bp
  • A to T, chromosome 8 at 19,126,506 bp
  • T to G, chromosome 8 at 24,818,136 bp
  • C to T, chromosome 8 at 27,198,485 bp
  • A to G, chromosome 8 at 116,933,396 bp
  • G to A, chromosome 8 at 125,761,557 bp
  • C to A, chromosome 9 at 44,973,584 bp
  • C to T, chromosome 9 at 50,805,354 bp
  • A to G, chromosome 9 at 65,703,695 bp
  • T to A, chromosome 9 at 112,177,677 bp
  • T to C, chromosome 9 at 114,095,924 bp
  • T to C, chromosome 10 at 80,126,377 bp
  • T to C, chromosome 10 at 109,694,082 bp
  • T to G, chromosome 10 at 125,233,439 bp
  • G to A, chromosome 11 at 107,077,692 bp
  • A to G, chromosome 12 at 66,716,722 bp
  • T to A, chromosome 12 at 110,657,139 bp
  • T to C, chromosome 12 at 113,685,596 bp
  • C to G, chromosome 14 at 54,735,790 bp
  • A to G, chromosome 15 at 76,297,955 bp
  • A to T, chromosome 16 at 20,707,825 bp
  • A to T, chromosome 16 at 38,549,416 bp
  • T to A, chromosome 16 at 81,512,996 bp
  • T to C, chromosome 17 at 28,724,824 bp
  • A to T, chromosome 17 at 55,483,017 bp
  • G to A, chromosome 18 at 5,759,007 bp
  • T to A, chromosome 18 at 24,683,212 bp
  • T to G, chromosome 19 at 4,195,055 bp
  • A to T, chromosome 19 at 34,648,513 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4514 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041588-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.